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Cell-free DNA

Product catalog summary

Overview
Zinexts offers DNA extraction kits that provide ultra-high and consistent recovery rates for short fragmented DNA. These kits are scalable across a wide range of sample volumes and can process samples to targeted DNA in under an hour, making them suitable for downstream applications, particularly Next-Generation Sequencing (NGS).

Specifications and Performance
The extraction kits have been tested with urine and plasma samples spiked with short-fragmented DNA (~150 bp) in various concentrations. The performance was confirmed using qPCR, demonstrating excellent sensitivity and reproducibility even with low amounts of circulating free DNA (cfDNA).

Urine cfDNA Extraction
Tests involved processing 5 ml urine samples with a 10-fold serial dilution and an elution volume of 50 µl. The kits showed outstanding scalability across sample volumes ranging from 4 to 15 ml. When compared to a market-leading product, Zinexts' kits matched the high and consistent recovery rates.

Plasma cfDNA Extraction
Using plasma samples with a 10-fold serial dilution, the Plasma cfDNA Extraction Kit demonstrated superb reproducibility and very high recovery rates, even at low cfDNA levels. The performance was comparable to that of a market-leading brand.

Conclusion
Zinexts' DNA extraction kits provide high performance and scalability, making them a competitive choice in the market for cfDNA extraction from both urine and plasma samples.

Catalog excerpts

Extraction Kits Ultra high and consistent recovery rate of short fragmented DNA Excellent scalability across a wide range of sample volumes From primary sample to targeted DNA in < 1 hour Perfectly suitable for any downstream application, especially NGS

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We processed 5 ml urine samples, spiked with short-fragmented DNA (~150 bp), in a 10-fold serial dilution of 200, 20, 2 and 0.2 copies/µl, and with elution volume of 50 µl. We confirmed the extraction performance by qPCR, using 2 µl of template. Excellent sensitivity and reproducibility of cfDNA extraction from urine, even with low amount of cfDNA present Outstanding scalability of the cfDNA extraction process from urine, across a wide range of sample volumes (4 – 15 ml) We did the comparison, extracting cfDNA from eight 4 ml urine samples with the Urine cfDNA Extraction Kit on the MagPurix instrument,...

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All Zinexts Life Science Corp. catalogs and technical brochures

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