MS 100R
2Pages

Catalog excerpts

Beads Cell Disrupter / Micro Homogenizing System Micro Smash™ Refrigerated type Cooling time inside the disruption chamber Digital Biology

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DNA extraction from yeast cells; S. pombe Proteinase K Method vs Beads Cell Disrupter MS-100 (l)In preparation for examination, yeasts collected from the culture broth at an OD660 of 0.20 were mixed with buffer. (2)Perform following three methods for cell disruption, two processes a. Processed wilhoul beads {as a negative control) h. Processed with (he Proteinase K. Method c. Processed with MS-100, using I mm glass beads at 3,000rpm for 5 minutes (3)After disruption, each sample was phenol extracted, cthanol precipitated and elcctrophorescd on a 1 % agarose gel. Genomic DNA and RNA, which...

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