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Thermo Fisher Scientific - Industrial & Applied S
UltiMate 3000 Rapid Separation LC (RSLC) System

UltiMate 3000 Rapid Separation LC (RSLC) System

Product catalog summary

Overview: The UltiMate 3000 Rapid Separation LC (RSLC) system is engineered for ultrafast and ultrahigh resolution liquid chromatography, providing high throughput with extensive pressure range and high flow rates. It is compatible with both UHPLC and conventional LC methods, ensuring high peak capacity in short run times.

System Features:

Flow rates up to 5 mL/min at pressures up to 80 MPa (11,600 psi).
Injection cycle times as short as 15 seconds.
Temperature range from 5 to 110 °C.
Data collection rate of 100 Hz for all UV-Vis detectors.
Automatic monitoring and diagnostics via Chromeleon Chromatography Management System.

HPG-3x00RS RSLC Pump:

Operates at ultrahigh back-pressures and flows up to 5 mL/min.
SmartFlow eluent delivery system for precise flow and gradient delivery.
Supports fast separations with low gradient delay volumes.
Designed for quiet operation and improved robustness.

WPS-3000RS Autosampler:

In-line split-loop injection for precise sample delivery with ultralow carryover.
Peak area precision typically less than 0.07% for a 10 µL injection.
Supports a wide range of vial and wellplate formats.
Injection volume range from 0.01 to 100 µL, extendable to 500 µL.

TCC-3000RS Column Compartment:

Temperature range of 5–110 °C with efficient cooling and heating.
Supports up to six columns for automated method development.
Sensors for humidity and organic vapor with rapid leak detection.

RSLC Detectors:

Includes Diode Array Detector (DAD), Multiple Wavelength Detector (MWD), and Variable Wavelength Detector (VWD).
DAD-3000RS offers high-resolution detection with a 1024-element photodiode array.
Supports data collection rates up to 100 Hz.
VWD-3400RS provides outstanding linearity and low baseline noise.

Specifications and Performance: The system's performance specifications include absorbance stability (<0.5 mAU/h at 254 nm and 520 nm with water at 1.0 mL/min), linearity (<3% RSD and Corr. Coeff. >0.9995 up to 1.5 AU), and repeatability (<5% RSD at 2.5 AU for caffeine at 272 nm). The VWD-3400RS detector shows excellent linearity with absorbance values up to 3000 mAU for active pharmaceutical ingredients (API).

Software and System Features: Supported by Chromeleon software, the system offers intuitive graphical control, remote monitoring, automated routines, and advanced wellness monitoring. It includes AutoQ™ qualification routines and smart column switching wizards for enhanced usability and efficiency.

Column Features: Acclaim RSLC columns with stationary phases of 2 µm and 3 µm provide ultrafast, ultrahigh resolution chromatography with lower back pressures and excellent separation efficiencies, supporting a maximum reliability pressure limit of 80 MPa.

Ordering Information: The document lists part numbers for various system components, including solvent racks, pumps, autosamplers, column compartments, and detectors, along with contact information for Dionex Corporation offices worldwide.

Catalog excerpts

UltiMate 3000 Rapid Separation LC (RSLC) System-1

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:9.00pt "Arial", sans-serif; } .font1 { font:9.00pt "Times New Roman", serif; } .font2 { font:16.00pt "Times New Roman", serif; } UltiMate 3000 Rapid Sparation LC System The UltiMate® 3000 Rapid Sparation LC (RSLC) system provides ultrafast, ultrahigh resolution LC separations using high flow rates for increased throughput. Its unique combination of extensive pressure range, high flow rates, and ultrafast data collection facilitates high peak capacity in the shortest run times. Even separations of 10 peaks in 10 seconds are easily achieved. The system is ideal for high speed and high resolution chromatography. Precision-engineered parts and robust operation maximize reliability and instrument uptime. Acclaim® RSLC columns feature small particle sizes for efficiently resolving peaks, optimizing fast LC applications. System Features 驕 Ultrahigh performance with both UHPLC and conventional LC methods Flow rates up to 5 mL/min at pressures up to 80 MPa (11,600 psi) Օ Injection cycle times of 15 s Temperature range of 5 to 110 °C Օ Data collection rate of 100 Hz for all UV-Vis detectors (VWD, MWD, DAD) Automatic instrument monitoring uses advanced system wellness features with Chromeleon® Chromatography Management System Օ Automatic IQ/OQ/PQ via Chromeleon Increased system uptime with easy-to-use diagnostic features

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UltiMate 3000 Rapid Separation LC (RSLC) System-2

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:4.20pt "Arial", sans-serif; } .font1 { font:8.00pt "Arial", sans-serif; } .font2 { font:9.00pt "Arial", sans-serif; } .font3 { font:4.00pt "Times New Roman", serif; } .font4 { font:5.00pt "Times New Roman", serif; } .font5 { font:6.00pt "Times New Roman", serif; } .font6 { font:9.00pt "Times New Roman", serif; } .font7 { font:11.00pt "Times New Roman", serif; } HPG-3x00RS RSLC Pump The HPG-3x00RS Rapid Sparation binary pump works at ultrahigh back-pressures and flows of up to 5 mL/min. It is designed for high speed, high resolution...

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UltiMate 3000 Rapid Separation LC (RSLC) System-3

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:4.20pt "Arial", sans-serif; } .font1 { font:5.00pt "Arial", sans-serif; } .font2 { font:6.00pt "Arial", sans-serif; } .font3 { font:8.00pt "Arial", sans-serif; } .font4 { font:8.00pt "Times New Roman", serif; } .font5 { font:9.00pt "Times New Roman", serif; } 23- Residual pulsation at 20.5 MPa: 0.07 MPa (0.34%) Residual pulsation at 12.5 MPa: 0.06 MPa (0.48%) 2.5 Minutes Flow: 1.5 mL/min Eluent: (A) Water (B) Methanol Gradient: Step gradient 0-30% methanol 20- MPa 15- 10- 5 0 1 2 3 4 5 6 7 Minutes 8 9 25405 Figure 3. Pressure diagram...

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UltiMate 3000 Rapid Separation LC (RSLC) System-4

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:5.25pt "Arial", sans-serif; } .font1 { font:8.00pt "Impact", sans-serif; } .font2 { font:8.00pt "Times New Roman", serif; } WPS-3000RS Autosampler The WPS-3000RS analytical autosampler uses the in-line split-loop injection principle, with the needle and sample loop as integral parts of the high-pressure flu-idic path. This injection principle provides highly accurate and precise delivery of the sample with ultralow carryover. WPS-3000RS Features ͕ Superior results with peak area precision typically less than 0.07% for a 10 injection...

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UltiMate 3000 Rapid Separation LC (RSLC) System-5

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:5.25pt "Arial", sans-serif; } .font1 { font:9.00pt "Impact", sans-serif; } .font2 { font:8.00pt "Times New Roman", serif; } .font3 { font:10.00pt "Times New Roman", serif; } Consistent results when transferring a method from one WPS-3000RS autosampler to another (accuracy typically better than ±0.5% at 20, 50, and 90 with a 100 syringe) Օ Sample thermostatting for optimal protection of your temperature sensitive analytes (4-45 °C with a ±2 °C accuracy in the sample vial) Peristaltic pump automatically removes water that may accumulate...

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UltiMate 3000 Rapid Separation LC (RSLC) System-6

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Impact", sans-serif; } .font1 { font:8.00pt "Times New Roman", serif; } TCC-3000RS Column Compartment Delivering a temperature range of 5-110 °C and offering eluent pre-column heaters, postcolumn coolers, and a variety of switching valves, the TCC-3000RS column compartment provides the full functionality required for fast, high resolution, and conventional chromatography. Large-area Peltier eleͭments in combination with a forced-air design ensure efficient cooling and heat-ing as well as homogenous and stable conditions...

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UltiMate 3000 Rapid Separation LC (RSLC) System-7

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:5.25pt "Arial", sans-serif; } .font1 { font:9.00pt "Impact", sans-serif; } .font2 { font:8.00pt "Times New Roman", serif; } .font3 { font:10.00pt "Times New Roman", serif; } Figure 12. An extensive range of user installable valve options support advanced column switching techniques. Figure 13. The column identification system enables automatic documentation of important column parameters for regulatory compliance and improved laboratory management. KEY SPECIFICATIONS Compartment Temperature Range: Post Column Cooler Cooling Capacity:...

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UltiMate 3000 Rapid Separation LC (RSLC) System-8

tr.row {} td.cell {} div.block {} div.paragraph {} .font0 { font:8.00pt "Impact", sans-serif; } .font1 { font:8.00pt "Times New Roman", serif; } RSLC Deteotors The UltiMate 3000RS UV detector family consists of a Diode Array Detector, a Multiple Wavelength Detector, and a Variable Wavelength Detector. The DAD-3000RS is a high-resolution, 1024 element photodiode array detector with 1 nm resolution. It features noise and drift performance previously available only in forward optic detectors. DAD-3000RS Features ͕ Deuterium and tungsten lamps for low baseline noise, high signal intensity, and application...

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