UltiMate 3000 Proteomics MDLC System

UltiMate 3000 Proteomics MDLC System

UltiMate 3000 Proteomics MDLC System

Product catalog summary
Innovative Technologies
The UltiMate 3000 Proteomics MDLC system offers a wide range of flow rates, from analytical to nano LC, with precise flow delivery and electronic monitoring. It includes factory-calibrated flow splitter cartridges, dual micropumps, and supports multidimensional separations and seamless MS integration.
Powerful Applications
Ideal for MS proteomics and bioanalytical methods, the system supports protein identification, peptide mapping, and microfractionation, with biocompatibility for post-translational modification studies.
System Features
Combining hardware, software, and chemistries, the system ensures optimal performance and reliability. It supports flow rates from 50 nL/min to 2.5 mL/min, maintaining constant flow rates regardless of mobile phase compositions or backpressures.
Flow Rate Flexibility
Interchangeable split-cartridges generate nano, capillary, and micro flow rates, with a chip identification system for accurate flow delivery.
Reproducibility and Reliability
SmartFlow™ Technology ensures pulsation-free gradient formation and superior gradient accuracy, enhancing performance and reliability in LC/MS proteomics.
Optimal Columns
Acclaim PepMap™ and PepSwift™ columns enable efficient protein and peptide separations, suitable for advanced chromatography applications.
Software Integration
Chromeleon Management provides automated system control and data analysis, while DCMSLink offers single-point control for LC/MS integration.
Biocompatible System Components
An iron-free, inert version is available for 100% biocompatibility, supporting sensitive protein and peptide analysis.
Off-Line Multidimensional LC
Offers flexibility in column dimensions and mobile phase selection, involving sample injection, first-dimension LC separation, and second-dimension LC separation with MS detection.
Specifications
The chromatographic process involves a loading solvent of water with 0.05% HFBA, flow rates of 2.5 µL/min for analytical and 20 µL/min for loading, and a gradient from 10% to 70% B over 15 minutes. UV detection is at 214 nm with a 3 nL flow cell.
Procedures
Describes a 2-D retention map process with ion-exchange separation in the first dimension and 2-D reversed-phase separation in the second.
Corporate Information
Dionex Corporation is headquartered in Sunnyvale, CA, with global sales and service contacts.
Quality and Trademarks
Dionex products are developed under an ISO 9001 Quality System, with trademarks like Analyst, Hystar, and MSQ.
Training and Support
Dionex provides worldwide training and support, with facilities equipped for advanced training and application development.
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