Innovative Technologies
The UltiMate 3000 Proteomics MDLC system offers a versatile flow rate range suitable for various applications, from analytical to nano LC. It features easy flow rate configuration, precise flow delivery as low as 50 nL/min, and electronic monitoring of nanoflow delivery. Key components include factory-calibrated flow splitter cartridges, dual micropumps, and multidimensional separations with different column formats. The system also supports microfraction collection and re-injection, seamless MS integration, and offers a biocompatibility option.

Powerful Applications
This system is designed as a front-end separation system for MS proteomics and bioanalytical methods, aiding in protein identification, peptide mapping, and microfractionation. It is biocompatible, making it suitable for studies on post-translational modifications.

System Features
The UltiMate 3000 Proteomics MDLC system integrates hardware, software, and chemistries to ensure optimal performance, reliability, and user-friendliness. It supports flow rates from 50 nL/min to 2.5 mL/min, maintaining constant flow rates regardless of mobile phase compositions or backpressures. The modular design allows customization for various applications.

Flow Rate Flexibility
Interchangeable split-cartridges enable the generation of nano, capillary, and micro flow rates, facilitating easy scaling. The split-cartridge chip identification system reduces manual handling.

Reproducibility and Reliability
UltiFlow technology provides active flow control with electronic monitoring, ensuring reliable peak timing and reproducibility across injections. The system supports a wide range of applications with high reproducibility and minimal carry-over.

Optimal Columns for High-Resolution LC
Using Acclaim PepMap™ and PepSwift™ columns, the system achieves efficient protein and peptide separations, easily integrated with MS. It supports advanced configurations for sample clean-up and preconcentration.

Software Integration
Chromeleon software offers intuitive wizards for automated start-up, system control, and data analysis. DCMSLink provides single-point control for LC/MS integration, compatible with various MS platforms.

Biocompatible System Components
The system is available in an iron-free, inert version for complete biocompatibility, supporting sensitive protein and peptide analysis.

Off-Line Multidimensional LC
Off-line multidimensional LC provides flexibility in column dimensions and mobile phase selection, typically involving sample injection, first-dimension LC separation, fraction collection, and second-dimension LC separation.

Specifications
The chromatographic process specifications include a loading solvent of water with 0.05% HFBA, flow rates of 2.5 µL/min for analytical purposes and 20 µL/min for loading, and a loading time of 5 minutes. The gradient transitions from 10% to 70% B over 15 minutes, followed by 90% B for 2 minutes, and a 6-minute equilibration period. UV detection is at 214 nm using a 3 nL flow cell. The sample consists of WAX fractions, with a loop and injection volume of 20 µL, and the oven temperature is maintained at 60°C.

Procedures
The document describes a 2-D retention map process, with ion-exchange separation in the first dimension and 2-D reversed-phase separation of the corresponding fraction in the second dimension.

Corporate Information
Dionex Corporation is headquartered in Sunnyvale, CA, with contact information for worldwide sales and service across North America, South America, Europe, and Asia Pacific.

Quality and Trademarks
Dionex products are developed under an ISO 9001 Quality System. Trademarks mentioned include Analyst, Hystar, and MSQ, associated with various companies.

Training and Support
Dionex emphasizes customer success through training and support, managing service and application facilities worldwide. These facilities provide advanced training and application development capabilities, equipped with Dionex HPLC and ion chromatography instrumentation and updated software, offering chemists opportunities to learn new skills and discover innovative solutions.