Thermo Scientific EASY-nLC 1200 System

Peak performance made EASY Effortless ultra high performance for everybody A straightforward LC-MS solution • Optimized and integrated system for proteomics • Highest pressure rating available, ideal for ultra long column applications • Ease-of-use with intuitive system operation • Excellent performance for every level of expertise “The EASY-nLC 1200 is more robust than the EASY-nLC 1000, and the higher pressure capabilities enable us to load and re-equilibrate at higher flow rates increasing our overall throughput.” Namrata Udeshi and Steve Carr, Broad Institute of MIT and Harvard All-In-One...

Thermo Scientific™ nanoViper™ fingertight fittings Full nanoViper flow path • Fast and reproducible nano connections • Easy, tool-free handling Switching valves New maintenance-free valves • Improved system reliability • Lower cost of ownership Nano LC pumps New 1200 bar pumps • Increase your analytical depth with longer columns • Higher throughput via faster loading and column equilibration Fast and simple instrument method set-up • Five-step wizard guides you through your method creation • Fully incorporated into Xcalibur for optimal use with Thermo Scientific™ mass spectrometers New software...

High precision and reproducibility with the EASY-nLC 1200 Consistent performance across a wide range of nano-flow rates 100 nL/min 90 min gradient The retention time precision for all three flow rates is better than 0.4% RSD. Excellent system stability at maximum pressure 42 The retention time precision is better than 1% RSD for 135 consecutive injections (run continuously over eight days). Conditions: • 75 cm Thermo Scientific™ Acclaim™ PepMap™ column • 300 nL/min gradient flow rate • 60 min gradient • 1180 bar sample loading and column equilibration • 900 bar gradient maximum pressure Consistent...

High throughput in deep proteomicsImprove your throughput by exploiting 1200 bar pressure 15 cm column Gradient Preparation time Preparation time “The ability to run at higher pressure enabled us to more rapidly load samples, hence dramatically improving sample throughput.” Thomas Kislinger, Princess Margaret Cancer Centre, University of Toronto Using longer columns will improve your protein coverage especially in combination with longer gradients. Conditions: 200 ng HELA digest Five injections per condition 75 cm and 50 cm Acclaim PepMap columns 300 nlTmin gradient flow rate 1180 bar sample...

Now available in 75 cm length for even higher separation and resolving power Exploit the enhanced pressure capability of the EASY-nLC 1200 with a column family that achieves higher peak capacities and improved protein identification LC-MS of 200 ng HELA digest using a 75 cm Acclaim PepMap column (240 min run time) The Acclaim PepMap family 1200 bar columns 75 pm i.d. in 15, 25, 50 and 75 cm length 50 pm i.d. in 15, 25 and 50 cm length • Providing more identifications by nano LC-MS • Highest sensitivity in TFA free nano LC-MS • Highest resolution through higher peak capacity The Acclaim PepMap...

The Thermo Scientific nano LC portfolio: The right choice for every lab EASY-nLC 1200 Operational simplicity and excellent performance UltiMate 3000 RSLCnano Versatility and unsurpassed precision Splitless nano flow System pressure ooo rerrormance Retention time precision Easily expandable to capillary and micro flow rates. More information available on thermofisher.com/nanoLCMS Not applicable, built-in heating for EASY-Spray column. May require an additional nano pump and corresponding application kit.

Thermo Scientific nano LC systems offer market leading performance and interface seamlessly with all Thermo Scientific nano ESI sources and MS families. EASY-nLC 1200 Operational simplicity and excellent performance Thermo Scientific™ Dionex UltiMate™ 3000 RSLCnano Versatility and unsurpassed precision Thermo Scientific™ Nanospray Flex™ ion source Thermo Scientific™ TSQ Quantiva™ triple quadrupole mass spectrometer Thermo Scientific’ EASY-Spray™ source Thermo Scientific™ EASY-Spray™ column Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer Thermo Scientific™ Orbitrap...