Thermo Scientific Dionex Corona ultra RS Charged Aerosol Detector Universal detection see what others miss consistent • sensitive • versatile

Going beyond HPLC The Problem—Detection No HPLC or UHPLC detector is perfect. UV detection is the most widely used technique, but it fails to detect compounds without chromophores. Other universal detectors do not combine application versatility with reliability. This results in detection gaps. The Solution The Thermo Scientific Dionex Corona ultra RS Charged Aerosol Detector is the solution. Charged aerosol detection delivers performance that refractive index (RI), low-wavelength UV, and evaporative light scattering (ELS) detectors simply cannot match. It measures analytes that other technologies...

Charged Aerosol Detection What makes any detector useful is its ability to accurately measure a wide range of analytes with consistent response. However, most detectors exhibit limitations. Often, one analyte responds more strongly than another, or may not respond at all. The Corona ultra RS detector measures charge that is imparted to analyte particles, with the charge being in direct proportion to the amount of the analyte in the sample. Measuring this charge is accurate and consistent, regardless of the analyte. The result is that the detector can quantify any nonvolatile analyte—this includes...

Consistent response and a wide dynamic range Consistent Response Detector Response for Nonvolatiles 10.7% RSD variation in CAD response among 1.0 µg samples by flow injection 250,000 200,000 150,000 100,000 50,000 Forced Degradation The Corona ultra RS detector is unique among universal detectors in that it allows quantitation across a range that exceeds four orders of magnitude. This wide dynamic range provides significant advantages for the simultaneous measurement of an analyte and low-level impurities in a single run. In the forced degradation example shown below, trace levels of degradant...

Enhanced performance Enhanced Sensitivity The Corona ultra RS detector provides an enhancement in sensitivity when used with UHPLC. Sub-nanogram levels of detection can be readily achieved, but there is more than just greater sensitivity. More importantly, the level of sensitivity is consistent across analyte types. Achieving New Levels of Sensitivity 500 pg of each sugar on column Glucose Sucrose Lactose Complementary to UV and MS Detection The Corona ultra RS is an ideal primary detector. However, when combined with UV, diode array detection (DAD), or MS, it provides an orthogonal and complementary...

Intuitive, simple to operate, and easy Ease of Use The Corona ultra RS detector is virtually plug-and-play. It uses touchscreen technology that is easy to navigate. There are few parameters to set, and the few options are displayed on a single screen. Instrumental parameters are explained on the screen to allow easy choice of settings. This simple, intuitive operation means the detector can be quickly installed, enabling you to quickly begin generating data. Diagnostics A diagnostic screen provides all the information necessary to assure that the detector is working correctly. In addition, values...

to integrate Easily Integrates with any HPLC System The Corona ultra RS detector can be used with any standard HPLC system, but is also ready for UHPLC without any modification. The detector is designed to integrate into any liquid chromatographic system, HPLC or UHPLC, from any manufacturer. Software drivers are available for the Thermo Scientific Dionex Chromeleon™ Chromatography Data System, as well as ChemStation®, EZChrom®, and Empower® software. The stackable design and rugged construction allow it to be placed anywhere in a system. There is no concern about moving or tipping the detector....

Application solutions Algal Oil Lipid Profile Fatty Acids HPLC is a powerful tool for lipid analysis. With UV detection, quantitation is made difficult Py differing relative response due to the wide variety of structures. Other universal detectors demonstrate a lack of response consistency making relative quantification difficult. With response independent of chemical structure, charged aerosol detection delivers consistent, predictable results with no optimization. Total Glycerides of Biodiesel The same detector can be used to assess purity or to perform in-process quality control of biodiesel....

Surfactants Surfactants such as Triton® X-100, which lacks a chromophore, can be easily measured with the Corona ultra RS detector. Unlike some other universal detectors, the wide dynamic range of charged aerosol detection technology lets you assay a full range of concentrations without overloading the detector. Surfactants Corona ultra RS (n = 5) 900 9 Other nebulizer-based detector (n = 1) 10 Detector overload Unique Data Algorithm 3 Response (peak area) Effect of Power Function 2 Response (peak area) The Corona ultra RS includes firmware to apply a power function to the data output, often...

Application solutions The Corona ultra RS detector makes it easy to determine the concentration and purity of any non- and many semivolatile active pharmaceutical ingredients (APIs) and excipients. With response independent of the chemical nature of the analyte, almost any API or compound used in the formulation can be measured with predictable response. Using the unique Thermo Scientific Acclaim Typical Pharmaceutical Agents 5 overlaid concentrations from 11–170 ng Product Characterization Pharmaceutical Analysis Trinity™ P1 column and the Corona ultra RS detector, the API, counterion, and impurity...

Biopharmaceutical Analysis Pegylated Protein Analysis of biopharmaceuticals present new challenges. Complex mixtures of large and small molecules, many without chromophores, can be analyzed using charged aerosol detection. PEGylated MAb PEGylation helps the biological stability of therapeutic monoclonal antibodies (MAbs) but measuring the residual PEG can be difficult. With predictable response irrespective of the analyte, charged aerosol detection can be used to measure residual PEG in PEGylated proteins. With its sensitivity and dynamic range, both low levels of the PEG impurity and the high...