ICS-5000 Modular, Metal-Free System Ideal for Bioanalyses The ICS-5000 Ion Chromatography system is the world's first capillary ion chromatography (IC) system on the market and provides an impressive combination of increased productivity, expanded capabilities, and improved performance. Modular versatility, functional integration, and superior performance culminate in the most advanced ion chromatography system available. The ICS-5000 Reagent-Free™ IC (RFIC™) system is the optimal liquid chromatography system for ion-exchange chromatography and biomolecule analysis. With a 100% metal-free system (autosampler, pump, flow path, and detector), the ICS-5000 system is able to overcome the limitations of traditional stainless steel or other metal chromatography systems affected by corrosive mobile phases. Metal flow paths can cause denaturation or absorption effects with some analytes, resulting in poor chromatography. Oxidized metals can poison columns, reducing separation performance and lifetime. The complete PEEK™ flow path of the ICS-5000 system, with its ability to resist pH Passion. Power. Productivity. values from 1–14, eliminates the possibility of system corrosion, and improves robustness. The all-PEEK pump can also perform quaternary gradients for demanding application needs (analytical scale). The ICS-5000 is the first IC system to allow separation on the analytical (4 mm and 2 mm) and capillary (0.4 mm) scale. Add to this, the ability to inject small sample volumes (down to 0.4 µL) from a variety of sample formats, including well plates; the versatility of multiple detection schemes (electrochemical, conductivity, mass spectrometric [MS] and UV-vis) even in series; and the ease-of-use of Always on, Always Ready, makes the ICS-5000 the most versatile IC system for bioanalysis.

Metabolomics The ICS-5000 system is an excellent platform for metabolomics. Unlike HPLC, ion chromatography separates the components that normally only elute in the void volume (early, unretained peaks). Ions and other highly charged species are easily separated and detected using suppressed conductivity detection (Figures 1 and 2). Eluents are converted to water by the suppressor, making the effluent completely MS compatible. The ICS-5000 system offers the advantages of separating anions and cations in one dual system (saving bench space). It allows sample volumes as low as 0.4 µL for precious...

Fermentation and Feedstocks Manufacture of biological products, such as biofuels and pharmaceuticals, increasingly utilizes fermentation. The ICS-5000 provides corrosion resistance for hydrolyzed samples, automation options for in-line matrix elimination and sample preparation, and electrochemical detection for determination of carbohydrates in feedstocks (Figure 4) and analaysis of alcohols, glycols, and carbohydrates in fermentation broths for in-process monitoring. The suppressed effluent from ion chromatography is also ideal for injection into a mass spectrometer for analysis of feedstock...

Related Dionex Applications Literature Dionex has an extensive library of methods and techniques for determining a wide variety of analytes important to the biotechnology and pharmaceutical industries. Below is a selected list of applications covered in this brochure. For more information, please visit www.dionex.com and click on the Documents tab, then on Application Notes and Updates, or contact your local Dionex representative. IC Solutions for Bioanalysis Analyte Alcohols, glycols Fermentation broths AN 188: Determination of Alcohols and Glycols in Fermentation Broths Using Ion-Exclusion...