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Technical NOTE 33

Technical NOTE 33
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Choosing the Right Purification System Chromatography Technical Note TN33 Teledyne Isco offers three purification system options for purifying organic compounds: ● CombiFlash® Rf+: A dedicated Flash purification system that can be operated in either normal or reverse phase mode. ● CombiFlash EZ Prep: A dual function system that switches between Flash and Preparative HPLC purifications with just a few clicks. ● ACCQPrep HP125: A dedicated Preparative HPLC purification system for difficult separations where impurities elute close to the target compounds. All systems are controlled by our intuitive PeakTrak® software which allows for easy migration from one model to another. Both the CombiFlash EZ Prep and the ACCQPrep HP125 exhibit excellent performance when separating compounds using RediSep® Prep columns packed with 5 µm particles, as well as other manufacturers’ preparative columns. The CombiFlash EZ Prep design is focused on Flash users who, at times, must resort to preparative HPLC to achieve their required purity. The system runs the same Flash column range as the CombiFlash Rf+ and in seconds can switch to preparative HPLC columns ranging from 10 mm to 50 mm ID. The pressure limit for flash is limited to 200 psi (14 bar) for compatibility with typical flash columns, but is increased to 3500 psi (240 bar) for use with preparative HPLC columns. CombiFlash Rf+ The CombiFlash Rf+ is compatible with Flash columns from 4 grams to 330 grams. Larger columns can be used at flow rates below their optimum. Typically, Flash is known for single use silica columns running in a normal phase mode. However, the use of reusable reverse phase media filled columns is gaining in popularity. Figure 2: EZ Prep System ACCQPrep HP125 The ACCQPrep HP125 is optimized for maximum resolution between closely eluting compounds. The flow rate range allows the user to run columns ranging from 4.6 mm to 50 mm ID. The pressure limit of 6000 psi (414 bar) supports the latest in HPLC media technology entering the market with particle diameters under 5 µm. Figure 1: CombiFlash Rf+ System

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Chromatography Technical Note TN33 a. Dolan, John W. LCGC North America 2006, 24 (5), 458 - 466. Test Probes: Methylparaben Ethylparaben Propylparaben Butylparaben Technical Comparison The following table highlights some of the key features for the different systems to aid in selecting the system most aligned to your needs. Performance Comparison Figures 4 and 5 are meant to act as an aid in making the decision on which system is right for your application. The chromatographic conditions for both Figures 4 and 5 are: Minutes Figure 5: ACCQPrep HP125 From these gradient runs you can see: •...

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