PRODUCT DATA y o u r Density Gradient Separation Programmable Density Gradient Fractionation System Density gradient centrifugation is used for the gentle isolation of cellular ribosomes, RNA, DNA, and organelles. SEPARATION SCIENCE Density Gradient Fractionation Low pressure LC instruments Automated Organic Purification Prepare gradients and use immediately without overnight refrigeration. Hands-off fraction separation. Seals and punctures the bottom of most common centrifuge tubes. Zone broadening and mixing are minimized with upward bulk flow. Detect at all popular wavelengths. Making the Gradient Isco’s Programmable Density Gradient System reproducibly forms density gradients in centrifuge-ready condition. Samples can be immediately loaded onto prepared gradients and spun in a centrifuge. LC Bioseparation Systems HPLC Syringe Pumps Supercritical Fluid Extraction & Chromatography Fractionating the Gradient Once the spin is completed, the separated components are recovered in separate collection tubes. Fractionate and quantitate centrifuged zones with unmatched precision. The Isco Density Gradient System produces a continuous absorbance profile as the gradient is collected in precisely measured fractions. Programmable Density Gradient System Contact Isco or your Isco Dealer for further details and product demonstrations. Isco, Inc. • PO Box 82531 • Lincoln NE 68501 • Phone (402) 464-0231 • Fax (402) 465-3022 • e-mail: [email protected] Toll-free (800) 228-4373 (USA and Ca

PRODUCT DATA Fractionation is performed by introducing a dense chase solution into the bottom of the centrifuged tube, raising the gradient intact by bulk flow. Chase solution may be injected either by piercing the bottom of the tube or from the top with the optional cannula. Absorbance detector light path Fraction collector Soft rubber collar Centrifuge tube Precise, No-Fuss Gradients Using two solutions where A=water and B=50% sucrose (w/v) in water with blue food coloring to visualize the gradient, three 5% to 40% sucrose gradients were prepared. Isco Density Gradient Systems Isco offers two...