MICROCALORIMETRY: TA Instruments RS-DSC, DSC & ITC

Microcalorimetry Differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC) are powerful analytical techniques for in-depth characterization of molecular binding events and structural stability. Thermodynamic binding signatures provided by ITC not only reveal the strength of a binding event, but the specific or nonspecific driving forces involved, while structural stability profiles from DSC reveal strengths and weaknesses in higher order structure that define the behavior of individual domains and their interactions. The TA Instruments™ RS-DSC (Rapid Screening DSC), Nano...

TA INSTRUMENTS RS-DSC Rapid Screening-Differential Scanning Calorimeter High throughput thermal stability testing with differential scanning calorimetry For scientists in the demanding world of biologic drug development, understanding biomolecule stability under thermal variations is crucial to ensure product quality, and support regulatory approval. Short term thermal stability testing reveals a compound’s resilience to thermal stress, predicts shelf life, and ensures efficacy. The requirement for accurately measuring this in a high throughput environment is challenging without disrupting processes...

TA INSTRUMENTS RS-DSC TECHNOLOGY Groundbreaking Capabilities The TA Instruments RS-DSC redefines the landscape by simultaneously analyzing 24 samples, significantly scaling up from traditional single-sample approaches. It operates with less than 15µL per sample, a substantial reduction compared to conventional capillary DSCs, and generally provides a clearer picture of the thermodynamics of unfolding than differential scanning fluorescence (DSF). Microfluidic Technology: The Future of Precision and Convenience Equipped with cutting-edge MFCs (Micro Fluidic Chips), the TA Instruments RS-DSC is...

TA INSTRUMENTS RS-DSC SOFTWARE Advanced Data Analysis for Optimal Formulation Decisions The TA Instruments RS-DSC comes with NanoAnalyze™ Software, an advanced software engineered to handle the data output from 24 concurrent analyses. Features like automated baseline fitting and peak detection simplify the data review process, allowing labs to effortlessly derive meaningful formulation insights. The software not only streamlines data management, but also enables rapid identification of optimal formulations based on melting temperatures, facilitating a quicker development trajectory. TRIOS™ Guardian...

Data plotting and visualization tools enable researchers to intuitively understand complex datasets and identify trends and patterns. 21CFR Part 11 Compliance with TRIOS Guardian Software helps to ensure the integrity, reliability, and trustworthiness of electronic records and signatures, and helps to meet regulatory standards for digital documentation.

TA INSTRUMENTS RS-DSC Rapid Screening-Differential Scanning Calorimeter TA Instruments RS-DSC: Your Strategic Advantage • Enhanced Throughput: The TA Instruments RS-DSC enables the analysis of 24 samples simultaneously which significantly speeds up research and accelerates biologic drug market entry. Resource Efficiency: The TA Instruments RS-DSCs requires less than <15uL of sample, to ensure maximum material use and minimize cost. High Concentration Proficiency: The TA Instruments RS-DSC excels in testing an extensive range of sample concentrations and has a unique capacity for analyzing very...

Discover the TA Instruments RS-DSC: ACCELERATE your RESEARCH and CULTIVATE INNOVATION New Possibilities Designed for research scientists in the biopharmaceutical industry, the TA Instruments RS-DSC is more than just a new instrument; it's an evolution in thermal stability screening. Improve biologic drug development with TA Instruments RS-DSC – where efficiency, precision, and reliability meet. Parallel Analysis: Unique high throughput analysis enables up to 24 simultaneous measurements to accelerate research. Single-Use Microfluidic Technology: MFCs streamline operation by simplifying the characterization...

TA INSTRUMENTS RS-DSC APPLICATIONS Formulation Buffer Screening Thermal stability is a leading indicator of overall clinical success of a biologic drug product, and DSC is a primary tool implemented to characterize the effect of the solution environment on protein stability. Effects on protein stability can be reflected in small shifts in Tmax or in changes of up to tens of degrees resulting from altering variables such as pH, buffer, ionic strength, excipients, and detergents on protein stability. Designed with the needs of the biopharmaceutical industry in mind, the parallel throughput of the...

Baseline Subtracted (kJ/mol•K) Tmax at Increasing [lysozyme] 68 66 64 62 Concentration Dependence – Investigate stability changes in highly concentrated drug products The TA Instruments RS-DSC is uniquely designed to handle high concentration biologic drug samples, with a specialized focus on antibody drugs and antibody drug conjugates. With the growing success of antibody therapeutics, the pharmaceutical industry has increased interest in high concentration dosage forms that enable subcutaneous and ocular drug delivery. As such, formulations with concentrations of 50 – 150 mg/mL antibody are...

NANO DSC AND DSC AUTO DIFFERENTIAL SCANNING CALORIMETER The Nano DSC has the versatility and precision for characterizing molecular stability, determining high affinity ligand binding and deconvoluting multi-domain structures. The Nano DSC and Nano DSC Auto incorporate proprietary technologies and provide higher performance with greater sample throughput. Higher sensitivity, lower cell volume for greater performance Capillary cell design for analysis of samples that tend to aggregate or precipitate Built-in precision pressurizing system maintains accurate, constant pressure in the cells Solid-state...

The Nano DSC is designed for ultra-sensitive measure of heat absorbed or released by dilute in-solution bio-molecules as they are heated or cooled. The capillary cell design, solid-state thermoelectric temperature control and easy cleaning ensure the higher sensitivity and data reproducibility for a wide variety of applications. 300 µL active volume capillary cells for analyzing hydrophobic samples Easy, accurate sample loading with laboratory pipetteman Built-in, user-programmable pressurization system (up to 6 atm) Flexible data acquisition interface for easy experiment setup NanoAnalyze software...