2012 Micro Cal cover_Layout 1 7/10/12 11:29 AM Page 2

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:18 AM Page 48

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:18 AM Page 49 MULTI CELL DIFFERENTIAL SCANNING CALORIMETRY The MCDSC is a versatile, multipurpose microcalorimeter that provides continuous temperature-scanning, step-scanning or isothermal measurements on three samples and one reference simultaneously. The one milliliter sample capacity ampoules and microwatt sensitivity ensures maximum flexibility for identifying phase transitions in chemicals and biologicals, metabolic activity in cellular organisms and thermal events occurring in complex reactions of liquids and/or solids during admixing. The...

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:18 AM Page 50

Short Term Noise Level 0.2 uW Scan Rate 0 (isothermal) to 2 °C/minute Response Time 90 second time constant or 9 minutes for 99% response Heat Measurement Method Heat flux

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:18 AM Page 52 No other DSC on the market offers more flexibility for studying thermal stability of almost any sample as a function of temperature or time. The calorimeter exploits ultra-sensitive Peltier technology for both temperature control and as sensors to detect heat effects. Peltier cascades allow precise temperature control for isothermal operation and reproducible scan rates up to 2 ºC per minute. The Peltier sensors give true microwatt detection independent of scan rate. Equipped with four removable cells, this is truly a workhorse instrument....

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:18 AM Page 53 Standard Ampoules High Pressure Ampoules Probe Accessible Ampoules Batch Reaction Ampoules Hastelloy®, 1.0 ml volume, 11mm diameter, 5mm depth Hastelloy®, 0.5ml volume, Kalrez® O-rings, max pressure 6000psi Standard Ampoule with special connections for pressure measurements, and connecting to spectrophotometers, mass specs, and gas chromatographs. Special Hastelloy® cells with two separate, sealed compartments for studying the reaction (and heat of reaction) between solids, liquids, and vapors.

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:18 AM Page 54 MC DSC Applications Drug-Excipient Compatibility 10 Reaction Heat Rate (µW/mg) Because of the large sample capacity and multiple cell design, the MC DSC can be used to rapidly determine the temperature dependence of rates of drug-excipient reactions. This figure shows the results from a continuous temperature scan of 155mg of a 1:1 mixture of aspirin and magnesium stearate. The rate of the endothermic reaction indicating incompatibility becomes measurable at about 50 ºC. Because of the wide-mouth (11mm) and depth (5mm) of the ampoules,...

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:19 AM Page 55 Shelf-Life Prediction by Step-Scanning 2500 Reaction Heat Rate (µW/g) In the step-scan method, the calorimeter temperature is rapidly scanned to a programmed temperature and then held isothermal for sufficient time (30-45 min) to allow for equilibration to accurately measure the steady-state heat rate, then stepped to the next temperature for another measurement of the isothermal heat rate. Step-scan measurements are particularly useful for accurate determination of activation energies and temperatures at which reaction mechanisms change....

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:19 AM Page 56 MC DSC Applications Temperature Dependence of Metabolic Rate In many organisms, metabolic heat rate is equivalent to the oxygen uptake rate, but heat rate is much easier to measure in cell cultures, tissues, and small organisms. Data on respiration rate as a continuous function of temperature is necessary to optimize conditions for cell cultures, for prediction of the effects of climate change on organisms and ecosystems, for selection of cultivars of crop plants for optimum productivity, and for prediction of the invasive potential...

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:19 AM Page 57 Determination of the temperature dependence of reaction rates is necessary for establishing proper storage, manufacturing, and compounding conditions for foods, drugs, cleaners, and industrial chemicals and products. The rates of reactions in diverse materials can be rapidly determined as a continuous function of temperature with the MC DSC. Continuous scanning is particularly useful for rapidly establishing the optimal temperatures for more definitive, but much slower, determinations of the identity of reactions and of rate laws by...

Micro Brochure 2012 WHITE_Layout 1 7/10/12 11:19 AM Page 58 REFERENCES Garbett, N., DeLeeuw, L. and J.B. Chaires. MCAPN-2010-04. High-throughput DSC: A Comparison of the TA Instruments Nano DSC Autosampler System™ with the GE Healthcare VP-Capillary DSC™ (2010) Demarse, N. and L.D. Hansen. MCAPN-2010-01. Analysis of Binding Organic Compounds to Nanoparticles by Isothermal Titration Calorimetry (ITC). (2010) Quinn, C.F. MCAPN-2010-02. Analyzing ITC Data for the Enthalpy of Binding Metal Ions to Ligands. (2010) Quinn, C.F. and L.D. Hansen. MCAPN-2010-03. Pressure Perturbation Calorimetry: Data...

2012 Micro Cal cover_Layout 1 7/10/12 11:29 AM Page 4

2012 Micro Cal cover_Layout 1 7/10/12 11:28 AM Page 1 © 2012 TA Instruments. All rights reserved.