Microcalorimetry
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MC DSC Technology 21 Applications 23 ISOTHERMAL CALORIMETRY TAM III 29 Technical Specifications 30 TAM 48 31 Technical Specifications 32 TAM AIR 33 Technical Specifications 34 TAM Technology 35 Nanocalorimeter 39 Microcalorimeter 40 Minicalorimeter 41 Multicalorimeter 42 ITC Accessory 43 Perfusion Calorimetry 45 Percision Solution Calorimetry 46 TAM Ampoules 47 Applications - Pharmaceuticals 49 Applications - Material Science 51 Applications - Life Science 53

The TA Instruments Nano Series calorimeters represent the highest sensitivity for the investigation of biological samples. The Nano ITC is focused on the measurement ligand binding and reaction kinetics, with increased sensitivity and lower detection limits to accommodate lower concentrations than previously possible.

The TA Instruments Nano ITC2G is engineered specifically for binding and kinetics studies on purified dilute biological samples of limited availability. With the Nano ITC2G, heat effects as small as 100 nanojoules are detectable using one nanomole or less of biopolymer. The Nano ITC2G uses a solid-state thermoelectric heating and cooling system to precisely control temperature, and a unique removable syringe assembly for efficient and accurate delivery of titrant. The true isothermal power compensation design of the Nano ITC2G delivers the fastest response time commercially available, 12...

Low Noise Level 0.004 ìWatt Baseline Stability 0.02 ìWatt/hr Temperature Stability 0.0002 ¢ªC at 25 ¢ªC Operating Temperature 2 to 80 ¢ªC Sample Size 1.0 mL Response Time 12 Seconds Cell Geometry Fixed Cylindrical Cell Composition Gold or Hastelloy Heat Measurement Type Power Compensation

productivity and efficiency by performing high-sensitivity analyses on nanomolar quantities of biomolecule. This is accomplished through a combination of a high-sensitivity calorimeter, accurate and stable temperature control, and efficient titrant delivery. The high-sensitivity cells of the Nano ITC2G are made from 99.999% gold or Hastelloy C to allow for the widest range of reagent chemistry. Their cylindrical shape makes cleaning easy and facilitates efficient stirring of the solution. The adiabatic shield of the Nano ITC2G is enclosed in a vacuum-tight chamber making the instrument less...

ISOTHERMAL TITRATION CALORIMETRY

thermodynamic and binding parameters as the incremental titration, but in a fraction of the time. Importantly, the Nano ITC2G performs either incremental or single i njection mode without any hardware or software modifications. 0 -100 -200 -300 -400 -500 -600 0 100 200 300 400 500 600 [mol titrant / mol titrate] Peak Area / ìJ 16 exo 14 12 10 8 6 4 2 0 -2 0 1000 2000 3000 4000 time /s Heat Rate / ì/W 10 200 300 400 500 600 700 800 900 1000 8 7 6 5 4 0 1 2 3 9 Time (seconds) n = 1.04 Ka = 1.12 x 106 M-1 ÄH = -73.4 kJ mol-1 Raw Data Fit Heat Rate (ìJ)

The TA Instruments Nano Series calorimeters represent highest sensitivity available for the investigation of biological samples. The Nano DSC is specifically designed determine the thermal stability and heat capacity of and other macromolecules in dilute solution, with versatility to allow the screening of ligands and pressure perturbation measurements.

The TA Instruments Nano Series calorimeters represent the highest sensitivity and unmatched performance for the investigation of biological samples. The Nano DSC is specifically designed to determine the thermal stability and heat capacity of proteins and other macromolecules in dilute solution, with the versatility and precision to perform molecular stability screening, ligand binding and pressure perturbation measurements. 11

Operating Temperature -10 to 130 °C optionally to 160 ¢ªC Pressure Perturbation (PPC) Built-in up to 6 atmospheres Scan Rate 0.001 to 2 ¢ªC/min Response Time 5 seconds Cell Volume 0.30 ml Cell Composition Platinum or Gold Cell Geometry Fixed, continuous Capillary or Cylindrical Heat Measurement Type Power Compensation

13 DIFFERENTIAL SCANNING CALORIMETRY

55 60 65 70 75 80 85 90 Temperature (°C) Molar Heat Capacity 2 5 10 25 50 100 400 Lysozyme Calorimetric van’t Hoff in cell (ìg) ÄH (kJ mol-1) ÄS (kJ K-1 mol-1) Tm (°C) ÄH 400 512 1.46 78.0 100 512 1.46 78.0 50 517 1.47 77.9 25 513 1.46 77.8 10 515 1.47 78.0 5 490 1.40 78.0 2 503 1.43 77.8 transducer. The Nano DSC uses an innovative dual-capillary design, operating in power compensation mode. The capillary design often delays the onset of protein aggregation until after the unfolding is complete. The result is a DSC measurement of unparalleled sensitivity, accuracy and precision. In fact,...

decreased substantially by high Zn2+ concentrations. shows that penetration of the drug changes the physical properties of the liposome. 30 40 50 60 70 80 90 Temperature (¢ªC) No zinc 1:3.5 1:14 1:70 Excess Heat Capacity 35 37 39 41 43 45 47 49 Temperature (¢ªC) gel-gel transition gel-liquid transition 5 hours 1 hour 0 Heat Flow (ìm) 0.02 0.04 0.06 0.08 0.10 0.12 -0.06 -0.08 -0.10

The TA MC DSC is designed for studying samples cannot or are not practical to investigate using thermal analysis instruments or ultra high sensitive calorimeters. The 1mL, removable, reusable ampoules allows measurements on solids, slurries, suspensions, liquids, or gases. The MC DSC is a versatile instrument with the sensitivity for making difficult measurements.