MICROCALORIMETRY: ITC & DSC
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Microcalorimetry Isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC) are powerful analytical techniques for in-depth characterization of molecular binding events and structural stability. Thermodynamic binding signatures not only reveal the strength of a binding event, but the specific or nonspecific driving forces involved. Structural stability profiles from DSC reveal strengths and weaknesses in higher order structure and define the behavior of individual domains and their interactions. The TA Instruments Affinity ITC, Nano ITC and Nano DSC provide the...

AFFINITY ITC and ITC AUTO ISOTHERMAL TITRATION CALORIMETER The Affinity ITC and ITC Auto are designed for the most challenging life science laboratory environments that require high sensitivity, high productivity and the most advanced ITC technologies. The Affinity ITC brings advanced engineering to all critical aspects of the measurement ensuring the highest quality ITC data. Features and Benefits: • AccuShot™ delivers the titrant to the right location for the best mixing • FlexSpin™ provides innovative slow speed stirring, efficient mixing and highest sensitivity • Fully automated,...

AFFINITY ITC TECHNOLOGY The Affinity ITC cell is optimized in shape, material, and volume to provide the greatest measurement accuracy over the widest range of sample chemistries. Choice of Cell Volumes: The Affinity ITC features two fixed-in-place calorimetric cells: a sample cell where injections take place and a matching reference cell. Two cell volumes are available: 1.0 mL (Standard Volume) and 190 µL (Low Volume). Automation is available in either configuration. Selection of cell volume depends on the range of binding constants to be measured (Kd: mM to low nM) and the availability of...

Solid-State Temperature Control & Power Compensation Operation. The Affinity ITC utilizes multiple solid-state thermoelectric elements for active heating and cooling of the sample and reference cells. Advantages of active heating and cooling: • Faster heating and cooling between temperature set points • Rapid equilibration at temperature set point • Active temperature control eliminates drift on long ITC experiments The absorption or evolution of heat as a result of a binding reaction is detected by the thermoelectric elements after which heater power is adjusted to maintain the temperature...

New FlexSpin technology dramatically improves one of the most important aspects of ITC experiments, mixing. Titrant Delivery Features and Benefits: • Revolutionary new paddle shape and its separation from injection system results in better mixing, sharper peaks and faster return to baseline • More efficient mixing and precise delivery of titrant reduces peak width up to 50% • Improved sample delivery system decreases equilibration times by 40% • Slower stir speeds (10X slower than competitive instruments) for highest sensitivity while protecting delicate structures • In conjunction with...

AccuShot' The precision and the location of the titrant delivery are critical to obtaining the highest quality ITC data. The AccuShot injection system has been completely redesigned to optimize these factors. AccuShot delivers the right amount of titrant in the right location, every time. Features and Benefits: • Injection system separate from stirring mechanism • Syringe needle positioned to deliver titrant at the top of stirring paddle for better mixing and sharper peaks • High precision stepper motor for the most accurate delivery of a 0.01 |_il_ to 250 |_il_ injection • Improved sample...

AFFINITY ITC AUTO I TECHNOLOGY What others have attempted TA Instruments has perfected. By combining automation and liquid handling technology prevalent in today's life science laboratories with the high performance Affinity ITC, TA has created the most powerful platform for automated, high throughput ITC experiments. Affinity ITC Autosampler Features and Benefits: • HPLC grade liquid handling • Small benchtop footprint to optimize space • Samples configured in two (2) 96-well plates • User-selectable temperature control (ambient down to 4°C) • Full sample pathway cleaning capability •...

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AFFINITY ITC AUTO INTELLIGENT HARDWARE POSITIONING Previous attempts at ITC automation commonly experience misalignment, broken and bent syringes and overall reliability issues. Intelligent Hardware Positioning eliminates these issues and ensures accurate and reliable placement of automated hardware on the Affinity ITC. Features and Benefits: • Alignment tabs ensure perfect positioning of the cell loading needle, stirring mechanism, and titrant injection cannula into the sample cell • Self-aligning arms are part of a proprietary design that provides safe, error-free positioning The...

The automated cleaning system engineered into the Affinity ITC Auto instrument ensures that the entire system is cleaned between sample titrations. This completely eliminates cross-contamination from one run to another. Cleaning Features and Benefits: • Dedicated wash/rinse stations for stirring/injection syringes and cell cleaning/filling • User-programmable cleaning routines can be saved and retrieved • Fully automated cleaning of the internal and external surfaces of key components, including autosampler transfer syringe and needle • Five user-selectable cleaning solutions • Complete...

Corrected Heat Rate (|o,J/s) Time(s) The Affinity ITC Auto provides unmatched sample reproducibility with the highest sensitivity and reliability. The figure shows twelve titrations performed with complete system cleaning performed between each titration. Data plots are offset for display purposes.

Affinity ITC Auto Cleaning Efficiency Two Buffer titrations performed before RNase-2'CMP titrations Complete system cleaning is user-programmable with Affinity ITC Auto instrument control software. Choosing from five (5) solvent ports ensures the entire sample path is clean. The buffer titrations before and after the protein-ligand titrations provide the highest confidence in the cleaning protocol for the Affinity ITC Auto.