MICROPIPETTES
108Pages

Pipette Cookbook 2018 P-97 & P-1000 Micropipette Pullers Rev. F

The Pipette Cookbook was written, compiled and created by Adair Oesterle with Sutter Instrument Company. A printed version of the Pipette Cookbook is included with all new P-97 and P-1000 Pullers and the pdf can found and downloaded for free through the Sutter web site. http://www.sutter.com/contact/faqs/pipette_cookbook.pdf Revised and expanded versions of the Pipette Cookbook are released every two to three years. Please contact Sutter if you have any recommendations, comments and/or corrections to offer. We welcome your feedback! Adair Oesterle at the Getty Museum – not pulling pipettes!...

TABLE OF CONTENTS Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Pg. 5-6 Using the Pipette Cookbook with the P-87, P-80/PC & P-1000 . . . . . . . . . . . . . . . . . . . Pg. 6 Sutter Capillary Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Pg. 7 Sutter Filaments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Pg. 8 Ingredients . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

Large Pipettes, 20µ to 200µ Tips, Methods on Breaking Back Tip, Glass-on-Glass Technique & Using the Ceramic Tile (CTS) . . . . . . . . . . Pg. 59-62 Aspiration Pipettes: Cell Selection, Cell Transfer, Single Cell Mechanics & Blastomere Biopsy. . . . . . . . . . . . . . . . . . . . . . Pg. 63-64 Aluminosilicate Glass and Custom Programs. . . . . . . . . . . . . . . . . . . . . . Pg. 65-68 ZIKA Research and Mosquito Egg Injections . . . . . . . . . . . . . . . . . . . . . Pg. 69-73 Installation of Box or Trough Filament - Review . . . . . . . . . . . . . . . . . . . Pg. 85-87 Eccentric...

Introduction The Sutter Instrument Pipette Cookbook is organized according to application and describes the required pipette needed for that application. While we have attempted to cover a wide range of applications and types of pipettes, it is possible we have yet to include what you might be looking for. Additional applications are occasionally added to new revisions of the cookbook and if you have suggestions for future revisions, please contact Sutter Instrument Company. At the beginning of each chapter you will find a general discussion of each application and a detailed description of...

If a program in this Cookbook does not produce the proper pipette, this might be a result of a poor alignment or mechanical adjustment on your puller. It is important to make sure your puller is in good working order. Be sure to check that the filament is perfectly centered over the air jet, the air jet is set 2 to 3mm below the base of the filament, the glass capillary is properly positioned within the filament, and the filament shape is correct. If you find that the program provided and the suggested changes to the parameter settings do not produce good results, please refer to page 11,...

Standard Wall Borosilicate Tubing (With Filament) Standard Wall Borosilicate Tubing (No Filament) Thin Wall Borosilicate Tubing (With Filament) Thin Wall Borosilicate Tubing (No Filament) For a list of our Aluminosilicate & Multibarrel Borosilicate Glass, please refer to our web site (www.sutter.com)

Filaments & Accessories Box Filaments P-1000, P-97, P-87, P80PC, P80C, PC-84, P-77B FB215B 2.0mm square box filament, 1.5mm wide…………………………………. FB220B 2.0mm square box filament, 2.0mm wide…………………………………. FB230B 2.0mm square box filament, 3.0mm wide…………………………………. FB255B 2.5mm square box filament, 2.5mm wide…………………………………. FB245B 2.5mm square box filament, 4.5mm wide…………………………………. FB315B 3.0mm square box filament, 1.5mm wide…………………………………. FB320B 3.0mm square box filament, 2.0mm wide…………………………………. FB330B 3.0mm square box filament, 3.0mm wide…………………………………. Accessories BX-10: Pipette Storage Box...

• PARAMETER SETTINGS = PROGRAM GLASS Within each Chapter describing a specific application, Slice Patch Recording for example, a specific glass type, a specific wall thickness, and filamented or non-filamented glass is recommended. Quartz glass is not discussed much in this cookbook because the filament-based pullers like the P-97 and P-1000 cannot melt Quartz glass, but it is mentioned (and recommended if you have a P-2000 Puller) in Chapter 13 - Zika Research and Mosquito Egg Injections. More information on the glass can be found in Chapter 14- Crazy Lab Lore. FILAMENTS The filament in...

PARAMETER SETTINGS The P-97 and P-1000 puller use the following parameters to control the melting and pulling of the glass: Each application described in the Pipette Cookbook will offer a starting program where the heat, pull, velocity, time/delay and pressure settings are suggested. Please see the SutterInstrument YouTube Channel to find the Scientists Empowering Scientists Webinar, “How to Make Better Pipettes”, which describes the parameters settings in more depth. The manual that came with your puller will also have a description of the parameter settings. A brief description of each...

in a longer tapered pipette. A higher delay value of 100-250 will provide more cooling to the glass before the hard pull engages, the glass will be more viscous, and this will result in a shorter tapered pipette. The delay mode of cooling is also most often used when pulling patch pipettes to deliver a longer duration of cooling to the glass which helps produce shorter tapered patch pipettes. As demonstrated on pages 30 and 31, a Delay mode of <1> with a Pull value of <0> results in short tapered pipettes. PRESSURE – This is the pressure of air used to cool the filament and the glass. The...