BV-10
1Pages

VIBRATION-FREE, MAGNETICALLY COUPLED BEVELING SURFACE ABRASIVE SURFACE OPTICALLY FLAT TO A HALF WAVE (250 nm) FINEST ABRASIVE SURFACE COMMERCIALLY AVAILABLE SYNCHRONOUS CLOCK MOTOR INSURES STABLE ROTATION RATE 7 POUND STEEL BASEPLATE ADDS ADDITIONAL DAMPENING INTEGRATED LED LAMP ROBUST MICROMANIPULATOR CONTROLS BEVEL ANGLE AND ADVANCEMENT (BV-10-D with optional 40X stereo microscope) BV-10 MICROELECTRODE BEVELER Elegant and simple to use, the BV-10 offers precision beveling of micropipette tips between 0.1 µm and 100 µm. The unique abrasive plate drive system is vibration free for greater control of the beveling process. Beveling can be accomplished very rapidly and produces consistent tip diameters using the techniques as described by Brown and Flaming, Science, August 1974, Vol. 185. Intracellular recording electrodes can benefit from beveling because of 1) a reduction in the tip diameter by creation of the sharp point on the electrode and 2) a lowered electrical resistance of the electrode due to the larger cross sectional area of the lumen. This greatly facilitates penetrating and holding very small or difficult cells. Microinjection needles also benefit from beveling by promoting entry into cells with minimal damage while at the same time enhancing the flow of material through the needle. The basic beveling system consists of a stationary pedestal, optically flat to a half wave (250 nm), surface mounted on a heavy baseplate. This serves as a bearing for an abrasive coated glass grinding plate, which is also flat to half a wave. The flat abrasive plate is coupled to a low vibration, slow-speed motor by means of magnetic fields to provide a wobble-free flat grinding surface. The abrasive plates are fabricated with a proprietary process which insures a consistent abrasive coating. BV-10-B1 Micropipette beveler basic system BV-10-C BV-10-B with electrode impedance meter BV-10-D BV-10-B with 40X stereo microscope BV-10-E BV-10-B with impedance meter and 40X stereo microscope 1 Includes micromanipulator, reference wick, reference wick holder, pedestal oil, degreaser, manual, and two abrasive plates of your choice. A 2-axis micromanipulator holds the pipette to be beveled and permits controlled advancement onto the abrasive surface. The bevel angle and speed of advancement are adjustable. An integrated LED lamp with a gooseneck enhances the beveling operation by providing sharp illumination of the abrasive plate and pipette. The basic system comes with two abrasive plates of your choice, a wick with holder (for wet beveling), pedestal oil, degreasing fluid, and manual. ® One Digital Drive • Novato • CA 94949 • Phone +1.415.883.0128 Fax +1.415.883.0572 • Web www.sutter.com • Email info@sutter.co