St ri v i n g f or t he Ultima te in Acc ura cy and E as y O per ability By combining new technologies with those cultivated over Shimadzu's long history, the Shimadzu spectrofluorophotometer has been reborn as the RF-6000. Combined with new LabSolutions RF software, designed for unrivaled measurement accuracy and easy operation, the RF-6000 offers the ultimate performance for a diverse range of customers' measurement needs.

W i d e Variety of Spectral Techniques Enhanced sensitivity and dynamic range enable fluorescence as well as bioluminescence, chemiluminescence, and electro-luminescence measurements. High-speed 3D scanning enables rapid acquisition of 3D spectra. Spectral-Corrected Excitation and Emission spectra can be scanned. Fluorescence quantum yield and Fluorescence quantum efficiency measurements are available. H i g h S e nsitivity, High Stability and High Speed Highest level SNR in its class: 1,000 or more (RMS) /350 or more (P-P) High-speed scanning of 60,000 nm/min minimizes scan time. 2,000 hour...

W i d e Variety of Spectral Techniques Supports Applications in a Wide Variety of Fields Pharmaceuticals Analysis of components in compounds Identification of artificial photosynthesis mechanisms Quality control of API or drug products Life Sciences Spectral properties of fluorescence probes Analysis of coumarin in light diesel oil Foods Quantitative analysis of additives Quality control of packaging Electrical/Electronics Environmental Evaluation of low-level contaminants in rivers and soils Spectral properties of fluorescent materials Measurement of quantum efficiency and quantum yield Analysis...

Electrical/Electronics Evaluating the Luminous Efficiency of Solid-State Semiconductor Materials A 100 mm diameter Spectralon integrating sphere unit was used to measure the fluorescence quantum efficiency of the light-emitting layer of a solid-state semiconductor material (tris(8-hydroxyquinolinato)aluminum) used in an organic EL device. Using the quantum efficiency measurement application of LabSolutions RF software allows determining the fluorescence quantum efficiency easily using intuitive software commands. The integrating sphere unit fits completely inside the sample compartment, eliminating...

Chemicals Long Wavelength Measurements The standard configuration can measure fluorescent wavelengths up to 900 nm, which means it can be used to research photosynthetic proteins for determining the mechanisms involved in artificial photosynthesis. In this case, a Thylakoid membrene solution was measured.*1, 2 The fluorescence spectrum shows that the system can accurately measure the fluorescence peaks in the long wavelength region. A spectral correction function, allowing the accurate measurement of spectral shapes in real time, is also included standard. Fluorescence Intensity Fluorescence...

Chemicals Potential for Identifying the Source of Minerals Calcite is a clear and colorless mineral consisting primarily of lime rock. Any impurities in the calcite may cause coloration. This example shows 3D fluorescence measurement (Excitation vs Emission) data for three types of calcite. Calcite A and B are clear and yellow whereas calcite C is clear and pink. 3D fluorescence patterns of calcite A and B were the same. In contrast, on the 3D fluorescence patterns of calcite C, there was a fluorescence peak at about 370 nm (EX; about 205 nm) and a strong fluorescence at about 430 nm (EX; about...

Pharmaceuticals Measuring Duloxetine Hydrochloride (USP) Fluorescence Intensity Fluorescence Intensity Duloxetine hydrochloride is a compound used as an antidepressant. In this example, an RF-6000 was used to measure duloxetine hydrochloride. The results indicated a lower limit of quantitation of 0.0007 μg/mL and a lower limit of detection of 0.0002 μg/mL, showing the ability of the RF-6000 to measure very low concentrations. Concentration (μg/mL) Fluorescence Spectra of Duloxetine Hydrochloride Calibration Curve Life Sciences Fluorescent Dyes for DNA Detection Specified complementary DNA can...

Foods Classifying and Identifying Types of Milk There are many types of milk products, such as raw or processed and those classified by fat content levels (low-fat, non-fat, and so on). These different types of milk products can have different 3D fluorescence spectra. In this example, three different types of milk products (A, B, and C) were used to measure 3D fluorescence spectra while varying the excitation wavelength. Samples were diluted by five times with distilled water. The results show that milk samples A and C have different fluorescence patterns. However, milk sample B has a fluorescence...

Environmental Measuring Oil in Water - ASTM D5412 The American Society for Testing and Materials (ASTM) testing standard D 5412 specifies testing for polycyclic aromatic hydrocarbons contained in water as oil. In this example, the target substance was separated from a solution prepared with a mixture of five polycyclic aromatic hydrocarbons using synchronized scan*. Figure 1 is a fluorescence spectrum of the mixture of polycyclic aromatic hydrocarbones. Figure 2 is a synchronized scan spectrum of Benzo[a]pyrene. Figure 3 is a synchronized scan spectrum of the mixture. The fluorescence peak of...

Environmental Trace Measurement of Chlorophyll Fluorescence Intensity The chlorophyll content is commonly measured when inspecting the water quality of rivers and lakes. Since the concentration of chlorophyll is low in rivers and other such waters, high sensitivity is required for measurements. Chlorophyll emits fluorescent light when exposed to light, but the light exposure causes the fluorescence intensity to gradually diminish. Therefore, fluorescence measurements that apply only a slight amount of excitation light are required. If the same chlorophyll solution is measured repeatedly with...

H i g h S e nsitivity, High Stability and High Speed High-Sensitivity Measurements High-sensitivity measurements can be performed with S/N ratios of 1,000:1 (RMS) or 350:1 (P-P). Improved lower limit of quantitation. Measures concentrations up to 1 × 10-13 mol/L (fluorescein). A redesigned optical system and signal processing system achieve the highest S/N ratio levels in its class. Even extremely dilute samples can be measured accurately. The RF-6000 can measure fluorescence spectra from fluorescein concentrations as low as 1 × 10-13 mol/L. Furthermore, due to an auto-gain control function that...