Analytical, Preparative Supercritical Fluid Chromatograph and Supercritical Fluid Extraction System

Unified Chromatography... Just another chromatographic technique... or the only technique you’ll need? Conventional LC/MS and GC/MS face these challenges... Unseparated peaks or low-intensity peaks Labor-intensive sample preparation Time-consuming post run processing Nexera UC received a 2015 “R&D 100” award from the American technology information magazine, “R&D Magazine” and a 2015 Pittcon Editors’ Gold Award. Nexera UC Prep received a 2019 Pittcon Editors’ Gold Award. SFE (Supercritical Fluid Extraction) : An extraction method using supercritical fluid. It is available as a pretreatment method...

Unified Unified Chromatography Chromatography Nexera UC and Nexera UC Prep ™ provide uncompromising solutions to these problems. Unified speed of analysis, sensitivity, and resolution Supercritical CO 2 enables highly efficient sample extraction and high-resolution chromatographic analysis. The result: improved sensitivity and throughput for multi-analyte analyses. High recovery rate of preparative purification with excellent operability Reliable small-volume fractionation is ensured through Shimadzu’s unique collection technology. Fully automated on-line sample preparation, analysis and fractionation...

Unified speed of analysis, sensitivity, and resolution Supercritical fluid chromatography (SFC) is a separation method that uses supercritical CO 2 as the mobile phase. The benefits of supercritical CO 2 are the low polarity similar to n-hexane, the lower viscosity, and the high diffusion coefficient, which offers potential advanced separation methods that are different from conventional HPLC separation. Thanks to this feature, SFC enables unique analyses, such as high-resolution chromatography, simultaneous analysis of compounds over a wide polarity range, and improvement of MS detection sensitivity....

Ideal for simultaneous analysis of compounds with a wide range of polarities SFC can achieve a wide variety of separation patterns. This allows comprehensive analysis of compounds over a wide polarity range not possible with HPLC. Different separation methods are generally used for fatty acids, which are typically analyzed by GC, and glycerides, which are typically analyzed by HPLC. However, because supercritical CO2 has properties similar to hexane, SFC is well-suited for analyzing compounds with different polarities, providing an ideal solution for the simultaneous analysis of fatty acids and...

Ensures high-throughput method development and scaling up Automatically performs a variety of method scouting processes The high-speed performance of SFC can shorten the time required for method scouting. The system automatically generates a large number of methods by utilizing combinations of up to 12 columns, four modifiers, and different ratios of modifiers to mobile phase. With the UHPLC / SFC Switching system, screening by UHPLC and SFC can be carried out in the same batch. Moreover, Method Scouting Solution, optional software for method scouting, makes it easy to set up multiple conditions....

• Method scouting for optimization of separation conditions and scaling up to preparative size For high-purity isolation, target peaks need to be adequately separated, for which the user must determine optimum column and separation parameter settings (method scouting). The Nexera UC chiral screening system and Method Scouting Solution can be used to screen columns more quickly and accurately (Step 1). Once the optimum column has been identified, smoothly scale up to preparative scale flow rates, preserving the peak separation while increasing the mass load (Step 2). *Preparative SFC: page 10...

Add fractionation capabilities to an analytical SFC system• Upgrade to an analytical fraction system By adding a fraction collector to an analytical scale SFC, small-volume fractionation is possible. A complete workflow, from method development to fractionation, can be carried out seamlessly on one system. Nexera UC Fraction collector FRC-40 SF • Small-volume fractionation is possible The patented gas-liquid separator, the LotusStream™, successfully reduces sample dispersion due to the volumetric expansion of CO2. It allows fractionation of small volumes, even into vessels such as 1.5 mL vials....

System configuration examples SFC-UV / SFC-MS System This is the minimum setup of Nexera UC and can replace both normal phase and reverse phase HPLCs. A wide range of analyte polarity can be covered by the combination of supercritical CO2 and modifiers (for example, MeOH). Hazardous organic solvents such as hexane or chloroform environmental impact by utilizing low-toxicity mobile phases and completing analyses in a shorter time. Both UV-Vis and MS detectors, such as the triple quad LCMS-8050, can be successfully coupled with this SFC system. Chiral Screening System This system is best for developing...

In purification using SFC, the target compounds are recovered in high concentrations in an organic solvent, which saves time not only during analysis, but also during post-run processing after preparative tasks are complete. The Nexera UC Prep maximizes fractionation output with its high recovery rates and ability to carry out continuous preparative work that further shortens the user's waiting time. Due to the low viscosity and high diffusivity of supercritical CO2, column backpressure for SFC is low even at high flow rates, which enables faster analysis without sacrificing column efficiency....

Stacked injection function eliminates waiting time Normal injection wastes time between peak elutions. Using the Nexera UC Prep’s stacked injection function, samples can be injected continuously without any waiting time, enabling more samples to be processed. Settings for this function can be specified easily in the dedicated software Prep Solution. Normal injection Waiting time 3× more injections Stacked injection (9 consecutive injections) Waiting time Chiral separation of warfarin Simple post-run processing Because most of the mobile phase is vaporized supercritical CO 2, only the organic...