Shimadzu Europa GmbH www.shimadzu.de Albert-Hahn-Str.6-10 47269 Duisburg Specification Sheet LC-2010 Integrated HPLC System LC-2010C LC-2010A Pump type Serial dual plunger, micro volume (10 ìL on primary side, 5 ìL on secondary) Flow rate setting range 0.001 ~ 5 mL/min. (1.0 - 35.0 MPa) Flow rate accuracy ± 1% (1 mL/min, 3.0 - 10.0 MPa, using water and fixed ambient temperature of 20°C, except during gradient solvent delivery), ± 2% (except for above conditions) or ± 2 ìL/min, whichever is greater Flow rate precision 0.075% RSD or 0.02 min SD, whichever is greater (0.05 - 2 mL/min, 1.0 - 35.0 MPa, methanol 20°C constant temperature) Number of solvents and setting range Up to 4 solvents 0 to 100%, 0.1% steps Concentration accuracy ± 0.5% (aqueous acetone/water, binary gradient, flow rate 0.1 - 2.0 mL/min, pressure 1.0 - 20.0 MPa) Pump LPGE Concentration precision ± 0.1% (aqueous acetone/water, binary gradient, flow rate 0.1 - 2.0 mL/min, pressure 1.0 - 20.0 MPa)

Shimadzu Europa GmbH www.shimadzu.de Albert-Hahn-Str.6-10 47269 Duisburg Specification Sheet: LC-2010 Integrated HPLC System (2/3) LC-2010C LC-2010A Injection Method Variable volume injection possible (without sample loss) Injection volume setting range 0.1 - 100 ìL (standard), 1 - 2000 ìL (optional) (0.1 - 0.9 ìL on 0.1 ìL steps, 1 to 2000 ìL in 1 ìL steps) 1 mL vial 350 1.5 mL vial 140 210 4 mL vial 100 MTP 4 96- or 384-well micro titer plates Sample capacity DWP 4 96-well deep-well plates Injection repeatability RSD < 0.3% (with 10 ìL injections) Carry over 0.01% (under napthalene analysis...

Shimadzu Europa GmbH www.shimadzu.de Albert-Hahn-Str.6-10 47269 Duisburg Specification Sheet: LC-2010 Integrated HPLC System (3/3) LC-2010C LC-2010A Light source Deuterium lamp Wavelength Range 190 - 600 nm Bandwidth 8 nm Wavelength accuracy ± 1 nm Wavelength precision ± 0.1 nm Drift 1x10-4 AU/h (250 nm air cell) Noise level ± 0.25 x 10-5 AU (for response of 1 s, 250 nm, air cell) Cell path length, cell volume 10 mm, 8 ìL (STD flow cell), 5 mm, 2.4 ìL (semimicro flow cell) UV Detector 2 wavelengths monitoring Any two wavelengths from 190 - 370 nm or 371 - 600 nm Display Backlit liquid crystal...