Imaging Mass Microscope iMScope TRIO

Imaging Mass Spectrometry Qualitative Analysis Optical Microscope Introducing the New Era of Imaging Mass Spectrometry Imaging mass spectrometry is a revolutionary new technology. The instrument is a combination of an optical microscope which allows the observation of high-resolution morphological images, with a mass spectrometer which identifies and visualizes the distribution of specific molecules. Superimposing the two images obtained based on these very different principles, has created a significant new research tool, the imaging mass microscope. The accurate and high resolution mass images...

Optical microscope Capture an optical microscope image Mass Spectrometer Mass spectra at multiple points Apply matrix to tissue section and irradiate with laser beam. Mass Spectrometry ■■■■■■■■■■■■■ =■■■■■ Multiplexed imaging Generate molecular distribution images (multiplexed imaging) based on ms signal intensity

Imaging Mass Spectrometry identifies what you see at the molecular level iMScope TRIO transforms your data from just "Observation" to "Analysis" Optical microscopes cannot determine which molecules are localized in the region of interest. On the other hand, the positional information of molecules is lost in mass spectrometric analysis, where sample extraction and homogenization is needed for sample pre-treatment. What if the qualitative analysis and localization of specific molecules and compositional and morphological observation could be obtained from a single analysis through microscope observation?...

Medical Research In medical research, the iMScope TRIO is particularly useful for identifying disease-related molecules (biomarker discovery) and rendering two-dimensional distributions of those substances (visualization). Also biological mechanism analysis and pathological research through identifying the location of target molecules (profiling) can be achieved.

Identification of Localized Molecules in Cancerous Mouse Liver Cells The iMScope TRIO makes it possible to obtain information regarding which molecules are localized within the region of interest in an organ, e.g. cancerous area. Until now, biomarker discovery has been carried out by LCMS or other mass spectrometry techniques for each organ. Bright Field Optical Image Fluorescence Optical Image Experiment Conditions Sample: mouse liver Matrix: 9-AA (aminoacridine, sprayed) Measurement pitch: 10 μm Laser diameter: 10 μm Measurement points: 200 × 200 (40,000 points) GSSG 4.0 Contents (μmol/g tissue)...

Profiling of Biological Tissue and Organ Cross Sections The iMScope TRIO, which incorporates database searching with MS and MSn data, can be applied not only to visualize the localization of a certain molecule, but also to identify the molecules relating to the cause of disease. Seminolipids in mouse testis Optical Image Experiment Conditions Sample: mouse testis Matrix: 9-AA (aminoacridine, vapor deposited) Measurement pitch: 10 μm Laser diameter: 10 μm Measurement points: 250 × 250 (62,500 points) Measurement time: about 3 hours The images show characteristic distributions of seminolipids (m/z...

Visualization of Lipid in a Mouse Cerebellum A wide variety of molecules relate to diseases states. Imaging mass spectrometry using the iMScope TRIO can detect a wide range of molecules within a defined mass range. Therefore, distribution information for several target molecules with different molecular weights can be determined simultaneously during a single measurement. Optical Image Experiment Conditions Sample: mouse cerebellum Matrix: DHB (vapor deposited) Measurement pitch: 10 μm Laser diameter: 10 μm Measurement points: 250 × 250 (62,500 points) Measurement time: about 3 hours *Ionization...

High Spatial Resolution Overlaying Images Pharmacokinetic Analysis Pharmaceuticals In drug discovery, researchers need to do a variety of pharmacokinetics research for many drug candidates in many situations. For instance, a wide range of spatial-resolution observation is needed, from the sub-cellular level high spatial resolution observation to mouse full body large area observation. The iMScope TRIO is useful for pharmacokinetics, pharmacological mechanisms, toxicity testing, and the development of ointments and cosmetics, owing ability to combine morphological observation from the optical...

High Spatial Resolution High Spatial Resolution Imaging High-resolution imaging offered by optical microscopes is required not only for pharmacokinetic analysis, but also for toxicity testing and toxicity mechanism Overlaying Images analysis. Analysis of the retina and skin requires imaging with high spatial resolution. Section with chloroquine administered (retina) Optical Image Optical Image Pharmacokinetic Analysis Scale bar: 50 μm MS/MS image at m/z 247.095 (10 μm pitch) Scale bar: 500 μm MS/MS image at m/z 247.095 (50 μm pitch) Experiment Conditions Sample: rat retina with chloroquine administered...

High Spatial Resolution Pharmacokinetic Analysis (whole body and tissue) Overlaying Images In pharmacokinetic analysis, the iMScope TRIO enables the distribution of unchanged and metabolized drugs to be simultaneously mapped in a single measurement, without any labeling. The laser diameter used during mass spectrometry imaging with the Pharmacokinetic Analysis iMScope TRIO is continuously variable from 5 to 200 μm, offering low to high spatial resolution. This helps ensure that analyses are performed as efficiently as possible. Section with famotidine administered (full body and kidney) Optical...

High Spatial Resolution Pharmacokinetic analysis of mouse with olaparib administered Overlaying Images Optical Image Pharmacokinetic Analysis MS/MS image at m/z 367.15 (precursor ion specified at m/z 435.15) Using a mouse administered with olaparib, the pharmacokinetic status of the tumor was Experiment Conditions Sample: tumor from mouse with olaparib administered visualized. In this way, imaging mass spectrometry is being utilized even in the clinical trial phase of drug discovery, rather than only during basic research. Matrix: α-CHCA Measurement pitch: 35 μm Samples provided by the Department...