Quick and easy monitoring of culture or fermentation processes High-speed Organic Acid Analysis Column: ™ Shim-pack Fast-OA In the fields of fermented food production and bio-industry, organic acid metabolites are monitored to control the activity of yeast or bacteria. The quantity of organic acids needs to be checked and the production environment adjusted accordingly in order to improve production and quality control, and these checks must be carried out in a timely manner. The Shim-pack Fast-OA is a column for the high-speed analysis of organic acids. It can separate multiple organic acids in a short time and supports real-time monitoring of their concentration levels. 6,000 Shim -pa ck Fast - O A (7.8 mm I.D. ×100 mm, 2 columns, flow rate 0.8 mL/min) 3 1 2 Phosphoric acid 1. Phosphoric acid 2. Citric acid 3. Lactic acid Citric acid Lactic acid （8.0 mm I.D. ×300 mm, 1 column, flow rate 0.8 mL/min) Monitoring of organic acid content in home-fermented yogurt Chromatograms of home-fermented yogurt A nalysis of s hort-c hain fatty a ci d s w i t h st ro n g re t e n t i o n i n u n d e r 1 0 m i n u t e s The Shim-pack Fast-OA column can also be used to analyze short-chain fatty acids with strong retention, of interest in the study of intestinal flora, in less than 10 minutes. 3 ■Analytical conditions Column : Shim-pack Fast-OA Mobile phase : 5 mmol/L p-toluenesulfonic acid pH buffer solution : 5 mmol/L p-toluenesulfonic acid 20 mmol/L Bis- Tris 0.1 mmol/L EDTA Flow rate : 0.8 mL/min Detection method : Conductivity detector (CDD-10AVP) 1. Pyruvic acid 2. Succinic acid 3. Formic acid 4. Acetic acid 5. Propionic acid 6. Butyric acid 7. Valeric acid 7 Chromatogram of a standard mixture containing seven organic

| Selective detection of organic acidsPost-column pH-buffering conductivity detection of organic acids is highly selective Culture mediums and food samples contain not only organic acids but also various impurities. With UV detection, the absorbance varies depending on the type of organic acid, so quantification may be difficult due to variations in sensitivity or the effects of impurities. A detection method combining post-column pH buffering and conductivity detection allows you to carry out highly-selective quantitative analysis of organic acids without worrying about overlapping contaminant...