Comprehensive Two-Dimensional Liquid Chromatograph

Nexera-e Comprehensive Two-Dimensional Liquid Chromatograph Novel Separation Method for Complicated Sample Matrices The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible theoretical plates are achieved for LC chromatography separation. The new Nexera-e enables separation of even the most complex mixtures, providing a new level of knowledge and understanding of sample analytes. This is especially beneficial...

Comprehensive Two-Dimensional Liquid Chromatograph

New Paradigm Shift in Liquid Chromatography Nexera-e Maximizing the full p g potential of two-orthogonal separation systems to g p y perform co mpre hens ive two-dimensional separation perf orm comprehensive two dime nsio nal sepa rati on pe rfor m comp rehe nsiv e tw o -di mens iona l se para tion Comprehensive 2D-LC is a new analytical methodology Unlike other conventional two-dimensional techniques the combining two independent separation modes orthogonally, Nexera-e provides complete separation as a result of the greatly increasing separation efficiency. The combination of combination of...

S e p a ra tio n fa r e xce e din g co nve n tio n al 1D - LC How many peaks are co-eluted under one peak? The more co-eluting compounds may be unable to be separated by complex the sample, along with a high percentage of similar mono-dimensional separation, the orthogonal compounds, means there is a high probability of co-eluting two-dimensional system will provide the best possible result. compounds found under single peaks. Even though the Unseparated peaks Second-dimensional separation Peaks separated with two separation systems Monodimensional separation First-dimensional separation Comparison...

Nexera-e Components Adoption of Nexera X 2 components of fering ultimate per formance Comprehensive 2D-LC requires excellent performance from requirements with its high-performance Nexera X2 each component. For example, the 2nd-dimensional separation components. For example, the solvent delivery unit, LC-30AD, system requires ultra-first gradient analysis with a high flow provides solvent delivery at a flow rate up to 3 mL/min with a rate and ultra-high pressure. Moreover, in order to analyze pressure limit of 130 MPa. In addition, the ultra-high-pressure differences between samples, excellent...

ChromSquare, the data analysis software for 2D-LC □ 2D Qualitative & Quantitative analysis using Contour Graphics Acquired data is converted to two-dimensional contour plotting using ChromSquare, the software for comprehensive 2D-LC analysis. A peak on chromatogram is recognized as spot or feature on the contour plot. The qualitative and quantitative data processing are performed for the target spot. LCxLC/MS data analysis of standard phospholipid sample Cupper left: Contour plot (magnified view); lower left: MS spectrum; upper right: Second-dimensional chromatogram; lower right: Contour plot...

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