Product Datasheet Unique, plug and play laboratory crossflow cassettes Product Overview  Rapid sample processing, with ready-to-use cassettes operated by a standard peristaltic pump  Plug and play simplicity, thanks to the unique flip-flow channels that ensure optimal flux  Flexible and modular scalability, with a broad choice of membrane, MWCO and single or multi use options for samples up to 5 L Vivaflow® brings the benefits of tangential flow filtration to research and development laboratories. Operated with a standard peristaltic pump, these ready-to-use cassettes eliminate the cost and complexity of process-scale systems. Ideal for the ultrafiltration and diafiltration of 0.1 to 5 L samples, the unique flip-flow channel design provides plug and play convenience, ensuring optimal crossflow velocities for fast concentr

Product Information The Vivaflow® range offers a choice of crossflow devices for scientists who need to reliably concentrate or re-buffer aqueous samples with initial volumes of up to 5 L. Unlike other crossflow cassettes on the market, Vivaflow® is a dedicated laboratory product that meets the demand for ease of use without requiring additional, non-standard equipment or significant process optimization. A choice of devices, operated with a standard peristaltic pump, achieve optimal results for every ultrafiltration need: Vivaflow® cassettes lend themselves to a multitude of ultrafiltration...

Technical Data Vivaflow® 50 Main housing Flow channel Membrane support Membrane seals and O rings Pressure indicator Flow restrictor Overall L | H | W Channel W | H Active membrane area Hold-up volume, cassette Non-recoverable hold-up Pump flow rate Maximum pressure Maximum temperature Operating Conditions * Pressure indicator is available separately (Order no. VFA020) as an optional accessory for Vivaflow® 50 ** SS = stainless steel **

Working Principle Convenient Diafiltration Vivaflow® cassettes contain an ultrafiltration membrane over which a sample is recirculated using a peristaltic pump (Figure 1). The thin channel flip-flow path provides high crossflow velocities with minimal pump speed requirements of 200 – 400 mL/min. A flow restrictor included with each cassette generates the optimal transmembrane pressure for concentration | diafiltration of the sample, whilst the filtrate is collected in a separate vessel. Ultrafiltration can be stopped as soon as the desired volume is reached by simply turning off the pump. The...

Components for Operating One to Six Vivaflow® 50 Cassettes Figure 4 Precise scale-up in process speed when using one to four Vivaflow® 50 cassettes. 4x cassettes The modular design of Vivaflow® 50 enables the operation of multiple cassettes in series and parallel (Figure 3 and Table 1). This increases the maximum throughput to up to 3 L and accelerates processing speed in proportion to the membrane area (Figure 4). Concentration Factor Time [hr:min] Figure 3 System components for operating multiple Vivaflow® 50 cassettes. Series interconnectors (Order no. VFA031) T-connectors (Order no. VFA030)...

Components for Operating One or Two Vivaflow® 50R | 200 Cassettes The maximum throughput of Vivaflow® 50R | 200 can Sample Feed also be increased and processing time reduced| by adding a second cassette to the setup (Figure 5 and Table 2). Figure 5 System components for operating two Vivaflow® 50R | 200 cassettes For Vivaflow® 50R: T-connectors to | from inlets | outlets (Order no. VFA030) For Vivaflow® 200: Y-connector to inlets (Order no. VFA005) Masterflex pump and Easy-Load pump head (Order no. VFP001 | VFP002 and VFA012 | VFA013) Table 2 Sample capacities and system components for operating...

Performance Performance Characteristics for Vivaflow® 50 Time to concentrate up to 20x at 3 bar inlet pressure, 20°C One Cassette Three Cassettes Recovery (Direct) Yeast, 1.0 mg/mL (S. cerevisiae) 0.2 µm PES Performance Characteristics for Vivaflow® 50R Time to concentrate up to 20x at 3 bar inlet pressure, 20°C 250 mL Initial Volume Average Flux Recovery (Direct) γ Globulins, 1.0 mg/mL (150,000 MW) 100,000 MWCO Hydrosart® Time to concentrate 1 L BSA (1 mg/mL) at 3 bar inlet pressure with 10,000 MWCO Hydrosart® One Vivaflow® 50R Cassette

Performance Characteristics for Vivaflow® 200 Time to concentrate up to 20x at 3 bar inlet pressure, 20°C 1 L Initial Volume Average Flux Recovery (Direct) BSA, 1.0 mg/mL (66,000 MW) 5,000 MWCO PES 5,000 MWCO Hydrosart γ Globulins, 1.0 mg/mL (average 150,000 MW) 100,000 MWCO PES Yeast, 1.0 mg/mL (S. cerevisiae) 0.2 µm PES Time to concentrate dilute solute from 1 L initial volume at 3 bar inlet pressure with 10,000 MWCO PES BSA, 0.001 mg/mL Time to concentrate BSA (1 mg/mL) from 5 L initial volume at 3 bar inlet pressure with 10,000 MWCO PES BSA, 1.0 mg/mL

Ordering Information Quantity Pump (240 V), Easy-Load pump head (size 16), tubing, 500 mL sample|diafiltration reservoir, cassette stand, pressure indicator, T connectors and series interconnectors Pump (115 V), Easy-Load pump head (size 16), tubing, 500 mL sample|diafiltration reservoir, cassette stand, pressure indicator, T-connectors and series interconnectors Vivaflow® 50 (includes 2x cassettes with filtrate tube, size 16 peristaltic tubing, flow restrictor and fittings, and 1x series interconnector) Vivaflow® 50 complete system Vivaflow® 50R (includes 1x cassette, filtrate tube, size 16...

Pump (240 V), Easy-Load pump head (size 16), tubing and 500 mL sample|diafiltration reservoir Pump (115 V), Easy-Load pump head (size 16), tubing and 500 mL sample|diafiltration reservoir Vivaflow® 50R  | 200 complete system Vivaflow® accessories Masterflex Economy Drive variable speed peristaltic pump (230 V) Masterflex Economy Drive variable speed peristaltic pump (115 V) 500 mL sample and|or diafiltration reservoir Masterflex Easy-Load pump head – size 16 Masterflex Easy-Load pump head – size 15 Vivaflow® 50 accessories for operating 2-6 cassettes T-connectors for running 2 stacks Series interconnectors...