Lab Ultrafiltration and Purification Products

General Information Major Uses for Ultrafiltration Sartorius Lab Ultrafiltration Devices Process Alternatives Membrane Performance Characteristics Membrane Selection Guide Protein and Macromolecule Concentration 9 Centrifugal Filtration Vivaspin® 500 10 ® Vivaspin 2 12 Centrisart® I 15 Vivaspin® Turbo 4 17 Vivaspin® 6 19 ® Vivaspin 15R 22 Vivaspin® Turbo 15 PES 24 Vivaspin® Turbo 15 RC 26 Vivaspin® 20 28 Vivaclear 32 Pressure-Fugation Vivacell 100 Tangential Flow Filtration Vivaflow® 50 Vivaflow® 50R Vivaflow® 200 Solvent Adsorption Vivapore® 5, 10 | 20 Ultrafiltration Membrane Filters from PES...

DNA Concentration Vivacon® 500 Vivacon® 2 Protein Purification Vivapure® Ion Exchange Protein Purification Products Virus Purification and Concentration Vivapure® Virus Purification and Concentration Kits Adenovirus Purification with Vivapure® AdenoPACK Kits Vivapure® AdenoPACK 20 Vivapure® AdenoPACK 100 Vivapure® AdenoPACK 500 Lentivirus Purification with Vivapure® LentiSELECT Kit Vivapure® LentiSELECT 40 Vivapure® LentiSELECT 500 Vivapure® LentiSELECT 1000 Application Notes 73 1. Desalting and Buffer Exchange with Vivaspin® Centrifugal Concentrators 74 2. Treatment of Vivaspin® Concentrators...

Major Uses for Ultrafiltration Solute concentration Solute fractionation or clarification Solute desalting or purification Ultrafiltration is a convective process that uses anisotropic semipermeable membranes to separate macromolecular species and solvents primarily on the basis of size. It is particularly appropriate for the concentration of macromolecules and can also be used to purify molecular species or for solvent exchange. Ultrafiltration is a gentle, non denaturing method that is more efficient and flexible than alternative processes Typical applications for ultrafiltration Concentration | desalting...

Sartorius Lab Ultrafiltration Devices Sartorius develops devices dedicated for optimising laboratory ultrafiltration processes for the purpose of minimising processing times and maximising recovery, reliability and robustness. In addition Sartorius are continually building technical and application support tools to help users select the optimum device and process for their sample type. Visit www.sartorius.com for more technical and application support material. Centrifugal concentrators Process Alternatives Cross flow Solvent absorption Sartorius offers a comprehensive range of process alternatives...

Membrane Performance Characteristics Sartorius offers an extended range of membranes to cover the great majority of ultrafiltration requirements. The following is a guide to selecting the most appropriate membranes according to their typical performance characteristics. Please note however, that membrane behavior and ultimate performance, largely depends on the specific characteristics of the solution being processed. Sartorius recommends that users experiment with alternative membranes in order to optimise their process performance. Polyethersulfone (PES) This is a low binding membrane that...

Membrane Selection Guide The advanced designs and low adsorption materials that characterise Sartorius products, offer a unique combination of faster processing speeds and higher recovery of the concentrated sample. Providing that the appropriate device size and membrane cut-off is selected, Sartorius products will typically yield recoveries of the concentrated sample in excess of 90% when the starting sample contains over 0.1 mg/mL of the solute of interest. The majority of any loss is caused by non specific binding both to the membrane surface and to exposed binding sites on the plastic of...

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Protein and Macromolecule Concentration Table of Contents Ultrafiltration Membrane Filters from PES 146…, CTA 145… and R

Vivaspin® 500 100 μL to 500 μL samples Vivaspin® 500 μL centrifugal filter units offer a simple, one step procedure for sample preparation. They can effectively be used in a fixed angle rotors accepting 2.2 mL centrifuge tubes. The legacy patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations, even with particle laden solutions. Technical specifications Vivaspin® 500 Concentrator capacity Swing bucket rotor Fixed angle rotor Dimensions Total length Active membrane area Hold-up volume, membrane and...

Performance characteristics Time to concentrate up to 30x [min.] at 20°C and solute recovery % Rotor Fixed angle Centrifugal force Start volume Aprotinin 0.25 mg/mL (6,500 MW) 3,000 MWCO PES BSA 1.0 mg/mL (66,000 MW) 5,000 MWCO PES 10,000 MWCO PES 30,000 MWCO PES IgG 0.25 mg/mL (160,000 MW) 30,000 MWCO PES 50,000 MWCO PES 100,000 MWCO PES Pack size Ordering information Starter pack (5 of each 5 k, 10 k, 30 k, 50 k, 100 k) 25 Protein Concentration  Centrifugal Filtration  Vivaspin® 500

Choice of membranes 0.4–2 mL samples The Vivaspin® 2 bridges the gap between the 500 μL and 4 mL centrifugal concentrators. This device combines the speed of the classic Vivaspin® products with low internal surface and membrane area for superior recoveries from very dilute solutions. Also unique to the Vivaspin® 2, is the choice of directly pipetting the concentrate from the dead stop pocket built into the bottom of the concen trator, or alternatively reverse spinning into the concentrate recovery cap which can then be sealed for storage. Both methods result in near total concentrate recoveries....

Performance characteristics Time to concentrate up to 30x [min.] at 20°C and solute recovery % Integral deadstop avoids risk of concentrating to dryness Fixed angle Centrifugal force Start volume PES, CTA, or Hydrosart® membranes; Filtrate container fits standard 15 mL tube carriers Direct pipette recovery or choice of reverse spinning concentrate into sample cap Aprotinin 0.25 mg/mL (6,500 MW) 3,000 MWCO PES BSA 1.0 mg/mL (66,000 MW) 5,000 MWCO PES 5,000 MWCO CTA 5,000 MWCO Hydrosart® 10,000 MWCO PES 10,000 MWCO CTA 10,000 MWCO Hydrosart® 20,000 MWCO CTA 30,000 MWCO PES 30,000 MWCO Hydrosart®...