Ambr® 250 High Throughput Perfusion Fast Track Intensified Cell Culture Processes

Ambr® 250 High Throughput Perfusion A Fully-Automated Bioreactor System for Intensified Cell Culture Process Development Ambr® 250 High Throughout Perfusion is a parallel bioreactor system for rapid development of scalable perfusion processes using 100 – 250 mL single-use bioreactors and a fully automated liquid handling platform. The system supports the typical range of hollow fibre cell retention applications, with up to 24 fully single-use perfusion bioreactors in parallel. This step change in perfusion process development capability dramatically accelerates optimization of intensified cell...

System Features The Ambr® 250 High Throughput Perfusion System Combines 12 or 24 Bioreactor Stations, Integrated Perfusion Pumps, Bleed and Permeate Collection with a Fully Automated Platform 1. Automated liquid handler The fully automated liquid handler carries out bioreactor sampling and liquid additions, significantly reducing manual operator interactions. For more information, please visit www.sartorius.com/en/products/fermentationbioreactors/ambr-multi-parallel-bioreactors/ ambr-250-high-throughput-perfusion 2. Integrated analytics The liquid handler transfers samples to a range of optional...

3. Bioreactor station array 12 or 24 bioreactor stations in parallel with full individual process control, offers convenient set-up and simplifies running of full DOE experiments. 4. Permeate and bleed flow control and collection system The system includes perfusion crossflow and permeate pumps, automated bleed functions, and both permeate and bleed fluid collection sets with collection bag and tubing management features. Also includes a walk-away permeate sampling system for samples up to 10 mL. 5. Integrated biological safety cabinet A class II biological safety cabinet designed to maintain...

1. Bioreactor perfusion features The single-use perfusion bioreactor includes a high efficiency multihole sparger capable of supporting very high cell densities. Two dip tubes allow flow of culture to and from the perfusion pump and filter, plus automated bleeding and bleed-to-level functions. Bioreactor Vessels Ambr® 250 High Throughput Single-Use Perfusion Bioreactors Include All Components Required for Perfusion Culture, Fully Assembled and Irradiated Single-use perfusion bioreactors significantly reduce staff time needed for set-up and cleaning, and enable rapid system turnaround, increasing...

2. Perfusion pump chambers Single-use pump chambers are used to drive the exchange of culture fluid between the bioreactor and the hollow fibre filter. The pump chamber assembly includes retaining clips for the cell retention filter and snaps quickly and neatly into place on the main system perfusion tower. 4. Pressure sensors Single-use pressure sensors connect quickly to the perfusion towers and continuously monitor pressure at the culture fluid inlet and permeate outlet, enabling online monitoring of transmembrane pressure. For more information, please visit www.sartorius.com/en/products/fermentationbioreactors/ambr-multi-parallel-bioreactors/...

Functions The System Performs Many Fully Automated Functions Bioreactor controller Three gasses per bioreactor with mass flow sensing of the mixed gas flow: O₂ CO₂ N₂/air Four positive displacement liquid pumps per bioreactor for high precision at low flow rates When operating with perfusion bioreactors, three pumps are available for reagent addition to the bioreactor and the fourth liquid pump is used to direct permeate flow from the perfusion filter to a collection bag For fed-batch operation using standard Ambr® 250 High Throughput bioreactors, all four liquid pumps are available for reagent...

Applications Ambr® 250 High Throughput Perfusion is capable of running in either perfusion culture or fed-batch mode, depending on the type of Ambr 250® High Throughput bioreactors installed. The system is able to cover a wide range of applications in biopharma development, including but not limited to perfusion process operations. Perfusion process clone selection Perfusion media development Perfusion process optimization Perfusion process characterization Perfusion process scale down model development

Scalability Single-Use from Cell Line and Process Development to Production Scale Geometrical similarity of vessel design Consistent mixing and gassing strategies High performance gas transfer and mixing Reliable single-use platforms Ambr® 250 High Throughput Perfusion BioPAT® Process Insights Included with the Ambr® 250 High Throughput system is a 1 year license of BioPAT® Process Insights software application. BioPAT® Process Insights enables predictive scale conversion and low risk process transfer between supported Sartorius bioreactors: Ambr® 15 and 250, Univessel® SU and Glass and Biostat...

Ambr® 250 High Throughput Perfusion Biostat® B-DCU with Univessel® Glass 1 - 10 L Also scalable to multi-use technologies - scaling up from 0.25 L to 2000 L Production Biostat STR®

Germany Sartorius Stedim Biotech GmbH August-Spindler-Strasse 11 37079 Goettingen Phone +49 551 308 0 USA Sartorius Stedim North America Inc. 565 Johnson Avenue Bohemia, NY 11716 Toll-Free +1 800 368 7178 or further contacts, visit F www.sartorius.com Specifications subject to change without notice. ©2024 Copyright Sartorius Stedim Biotech GmbH, August-Spindler-Strasse 11, 37079 Goettingen, Germany Status: 03 | 2024