Rotachrom LC-CPC Applications
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APPLICATIONS CENTRIFUGAL PARTITION CHROMATOGRAPHY

Isolation of an Antibiotic Oligopeptide (Cyclosporine A) Goal: Purification Cyclosporine A is a cyclic oligopeptide, with a lipid sidechain. This Active Pharmaceutical Ingredient (API) is produced by fermentation. The result of fermentation is usually a mixture of products with closely related structural properties. These are usually purified by classical purification methods: a combination of crystallization and preparative HPLC. Throughput: 22.4 g/h Purity: 99% Recovery: 70-90% Concentration [mg/ml] To avoid costly two-step purification methods, RotaChrom developed a CPC solution which...

Isolation of Digoxin from a Natural Extract CoI: Digoxin Goal: Isolation Concentration [mg/ml] Digoxin is a secondary cardiotonic glycoside isolated from the fermented foliage of foxglove (Digitalis lanata). High-value bioactive compounds are usually surrounded by compounds of similar structure. These are often difficult to be isolated in their pure forms. Digoxin’s mono- and bisdigitoxosides, digoxigenin and gitoxin were the main impurities that needed remediation. At the end of the study, we separated Digoxin directly from the crude extract. Preparative Separation of Diastereomers CoI:...

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