Continuous CPC
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WITH CENTRIFUGAL PARTITION CHROMATOGRAPHY Purified Solutions

RotaChrom's CPC platforms are quintessential applications when looking for a high-purity, high-capacity, cost-effective and environmentally conscious solution. RotaChrom, with its in-house research laboratory employing world class PhD chemists, engineers and leading business professionals on multiple continents, is the best partner in purification.

Batch Processing and Continuous Processing Nowadays, chromatography techniques evolved to a point where laboratory and industrial-scale separations are routine work. In centrifugal partition chromatography (CPC) separations, the possibility to work without silica gel and irreversible bondings, and the selection of suitable solvent systems are simplified with the availability of generic sets of solvent combinations described in various publications and books. However, continuous operation and easily scaling-up productivity can still be challenging. In a classic batch process, a specific...

Multiple dual-mode (MDM): An Advanced Operation Mode to Enhance the Resolving Power of CPC In liquid-liquid chromatography, we make the most of the liquid nature of the phases. By switching the roles and flow directions of these liquid phases during the separation process (known as dual-mode), we can artificially extend the length of the column and thus enhance the resolving power of the CPC system. Imagine we have a mixture of compounds A and B with very similar partition coefficients (Kd), and the rotor is initially filled with a 50/50 (v/v) upper (UP) and lower phase (LP). The separation...

Multiple dual-mode (MDM): An Advanced Operation Mode to Enhance the Resolving Power of CPC A system with two connected rotors, equipped with four pumps, allows for continuous or semi-continuous operation in a repeating process. In this setup, a mixture is constantly introduced between the two rotors, and two products (A and B) are collected one after the other at opposite “ends” of the two-rotor system. Each cycle consists of two steps (Fig.4. and Fig. 5.), similar to the ascending and descending elution steps in CPC separation modes. These steps differ in the liquid phase used as a mobile...

Case Study: Separation Of Salicylic Acid And Benzoic Acid Model Mixture In this experiment, a model mixture containing equal parts of salicylic acid and benzoic acid, with a total concentration of 50 mg/mL, was used. A 5 g sample of this mixture was injected into an rCPC instrument. The entire process, from injection to elution, took 20 minutes, with elution being collected in 1 ml/min fractions. Figure 10 shows the UV-chomratogram of the CPC separation recorded at 254 nm. Salicylic acid eluted between11-15 minutes, and benzoic acid between the 16-20 minutes. The distribution of salicylic...

Threefold Increase In Productivity Case Study: PA-Remediation of Red Clover The aim of the separation was to reduce the pyrrolizidine method was suitable for continuous injection, and so the alkaloid (PA) content below 400 ppb. Both methods successfully decreased the content of these alkaloids below the specification, while keeping the isoflavones content of the samples on the same level. Red Clover (Trifolium pratense) is another commonly used plant in traditional medicine, used to treat diseases such as osteoporosis, heart disease, arthritis, skin disorders like eczema, respiratory...

Separation of Diastereomeres With High Purity Case Study: Remdesivir Isolation Remdesivir is a phosphoramidate nucleotide prodrug approved for the treatment of severe COVID-19 infection in 2020. It contains a chiral phosphorous center with the current clinical compound being the SP-diastereomer (GS-5734). The pharmacological relevance of the RP-diastereomer, however, is considered also significant (Fig. 17.). The chiral stationary phase utilizing preparative HPLC method initially reported by an originator, is an expensive and hardly scalable step in the total synthesis of remdesivir. We...

RotaChrom’s Continuous Centrifugal Partition Chromatography (Continuous CPC) platform is a continuous, multiple dual-mode, dual-rotor CPC platform. It consists of two interconnected rCPC devices, a preparative unit, and the control unit. It’s highly scalable, adaptable to various sizes and requirements, and built with robustness in mind, meeting industrial standards for durability. This device is exceptionally versatile, making it adept at handling liquid-liquid chromatography. It incorporates an on-demand solvent handling system that can be tailored to specific industrial standards....

The two rCPC instruments provide the core of the separation unit containing the two rotors. The rCPC is RotaChrom's liquid-liquid centrifugal partition chromatography platform, designed to adhere to GMP regulations. rCPC Data Sheet Industrial valves o Designed for minimal maintenance and long-lasting performance. Three Membrane Pump Pulse-free operation of the pump, thanks to j three-membrane setup. High-quality oil o Suitable for applications in the food industry. Control Unit 12" HMI Interface o on-time monitoring of the Continuous CPC. Modular Device easy connectivity and expansion....

Method Editor Editing time-triggered event table-based methods (recipes) • On the method editor interface, the control parameters of the platform's devices (valves, flow rates, rotor rotational speeds) can be determined for each data row based on the time elapsed since the method started. • The control parameters that have been defined are easier to understand and verify with the help of the method diagram. Editing and running sequences (batches); possibility of continuous production • The created methods can be organized into sequences, and within a sequence, the number of repetitions of...

RotaChrom Purified Solutions hello@rotachrom.com www.rotachrom.com RotaChrom delivers innovative, sustainable and efficient chromatography to produce a variety of compounds for use in many different industries. Csillag street 2/A, Kecskemet, HU-6000 300 Spectrum Center Drive, Irvine, CA 92618, USA RotaChrom® is a registered trademark of Rotachrom Technologies in the USA and EU. All rights reserved.