ATUMtome Tap e Co llecting Ultramicrotome For High Resolution Array Tomography of Biological Tissues by Early Adopter Sophisticated Users* The ATUMtome’s tape collecting part of the system (ATUM) is under license from Harvard University where Professor Jeff Lichtman, MD, and his team designed the instrument to help collect ultrathin sections to help reconstruct the brain’s neural pathways -- a precursor to one day mapping the entire human brain. Thousands of ultra-thin sections with a thickness of 30nm can be automatically collected on 8mm wide Kapton tape for SEM imaging and subsequent 3-D reconstruction. Features & Benefits • Collects hundreds to thousands of sections on a continuous tape • Uses multiple resolutions for locating regions of interest, then zooming in for higher • Non-destructive to sample, with sections available for years into the future for resolution imaging • Allows correlative microscopy for localization processing, post-staining, immunogold labeling, correlative imaging -- and at any work pace desired. and then ultra-structural imaging • Prepares samples allowing short pixel dwell time for fast detection of images during EM • Section thickness usually range from 30nm to 5,000nm, with thinner sections possible. • Determines sample viability sooner in the process so you don’t waste valuable time cutting and imaging a problem sample. • Uses standard sample preparation techniques and resins • Charging effect on sections is manageable • Priced at a fraction of the cost of alternative 3-D imaging techniques * The ATUMtome is available only to sophisticated users during the Early Adopter Program. 4650 S. Butterfield Drive • Tucson, Arizona 85714 USA Phone: 520.745.0001 • Fax: 520.745.0004 • E-mail: [email protected] • www.rmcboeckeler.com © 2015 Boeckeler Instruments, Inc. Boeckeler is a registered trademark of Boeckeler Instruments, Inc., Tucson, Arizona. All other trademarks are the property of their respective owners. All specifications subject to change without notice.

Description The RMC ATUMtome is a unique system for collecting sections on a continuous tape. Typically, researchers mount resin-embedded specimens on the ultramicrotome, cutting serial sections that then float on a surface of the water in the diamond knife trough. The ATUMtome moves a continuous ribbon of tape through this water trough, automatically removing the serial sections from the water surface in sequential order. After the sections are collected, researchers mount the tape in strips on substrates like a silicon wafers or glass slides. The populated wafers are now ready for imaging with...

AT U M t o m e S y s t e m ATUMtome supplied complete with: • ATUM continuous tape feed mechanism with PC control software • PowerTome PT-PCZ • Air-activated anti-vibration microtomy table including ATUM attachment interface with x-y-z fine control positioning of tape/section pick-up head • Silent compressor • Environmental chamber • Anti-static device • Ergonomic lab chair • 4 mm diamond knife, 35 degrees for room temperature ultra-thin sectioning, mounted in large-cavity blue anodized holder • Water level control system • Wafer workstation • Start-up supply of Kapton tape • Four 4” diameter...