Catalog excerpts
Discover the CE you’ve been missing. Designed for STR analysis, the Spectrum CE System is built to streamline and support your forensic and paternity workflow needs. Spectrum CE System combines state-of-the-art STR analysis functionality with increased run flexibility, auto-analysis, user-friendly software, and the service and support you’ve come to trust from Promega. Spectrum is designed for use with existing 4-, 5- and 6-dye STR kits from Promega, Applied Biosystems and Qiagen. More Efficient Operation Choose from 8-capillary and 24-capillary array configurations. With four 96-well plate...
Open the catalog to page 2Spectrum has four 96-well plate positions, and provides continuous access to those plates. This means that you can run partial plates and add samples during a run—gaining scheduling flexibility while still maintaining productivity.
Open the catalog to page 3Advanced Multiplexing Capabilities: More Information from Challenging Samples Spectrum supports the next generation of PowerPlex® 8-dye STR Systems. These new PowerPlex® Systems enable amplification and detection of more loci from challenging casework samples, resulting in more meaningful analyses. Labs using the 8-dye systems can also expect more consistent results from variable database and reference samples due to the narrower range of product amplicon sizes.
Open the catalog to page 4The PowerPlex® 35GY 8C System allows co-amplification and fluorescent detection of the 20 CODIS Core Loci plus Amelogenin and DYS391 for gender discrimination. Penta D, Penta E and SE33 also are included to increase discrimination and allow searching of databases that contain profiles with these loci. Additionally, we've designed the layout to allow smaller amplicon sizes for these particularly informative loci: SE33, D21S11 and D2S1338. Ten additional Y-STR loci are included to assist with forensic casework on sexual assault evidence or to enable familial searching, a technique known for...
Open the catalog to page 5Designed to work exclusively on the Spectrum CE System, the PowerPlex® 35GY 8C System is compatible with both extracted DNA and direct amplification samples. The kit offers optimized protocols for various sample types, including direct amplification from FTA® cards and pretreated non FTA® card punches or swabs in both full-volume (25gl) and half-volume (12.5gl) reactions. The figure below shows results from a cotton swab buccal sample in which the DNA from that swab was amplified directly using a half-volume reaction. DNA from a buccal swab in Swab Solution was amplified with the prototype...
Open the catalog to page 6Detect Inhibitors with an IPC New in the 8-color PowerPlex® systems is the inclusion of an Internal PCR Control (IPC) to assess the presence of inhibitors, designed to minimize the need to repeat unusable or poor STR analyses which resulted from inhibited DNA. The IPC can provide more information about the sample, allowing you to make better decisions.
Open the catalog to page 7Compatible with the Kits You Already Love Spectrum CE System is the only CE to support 8-dye technology, but if your laboratory is satisfied with an existing 4-, 5- or 6-color kit you’ve already brought online, the Spectrum CE System has got you covered. You’ll be able to continue using those same multiplexes and still benefit from the increased throughput and flexibility that Spectrum CE enables. The following figures demonstrate the solid performance of the Spectrum CE System when running samples amplified with the GlobalFiler® PCR Amplification Kit and the PowerPlex® Fusion 6C System....
Open the catalog to page 8II II II 2800M Control DNA (1ng) was amplified with GlobalFiler® PCR Amplification Kit. Amplification products were separated using the Spectrum CE System.
Open the catalog to page 9We've designed the Spectrum CE System to provide simplified setup and operation. Related consumables, such as pre-filled buffer and polymer, will be offered in convenient, ready-to-use packaging. Complementary consumables including plates, septa mats, and formamide are also available for purchase. Spectrum uses Radio Frequency Identification (RFID) to track key consumables with minimal "hard stops” limited to situations where there is a risk of instrument damage. Spectrum Consumables Spectrum Capillary Array • 8-Capillary and 24-Capillary • RFID-tagged for automatic consumable recording...
Open the catalog to page 10Plate, Barcoded, Semi-Skirted, 96-Well • Barcoded 96-well plates for PCR, qPCR and CE • Compatible with most AB instruments, including sequencers Formamide, CE-Grade, 25ml • Highly deionized, Low conductivity, <5pS. • Used for electrokinetic injection on CE instruments Optical Plate Seals • Encapsulated ThermalSeal® Film for qPCR Reactions • Reduces the chance of well-to-well contamination and sample evaporation when applied to a microplate Strip Cap, 8-Well • 8-Strip domed caps for PCR plates and tubes to prevent evaporation and contamination • Certified RNase- and DNase-Free Septa Mat,...
Open the catalog to page 11One-Click Sample Tracking Spectrum features automatic recording of plate barcodes and auto-import of plate map files with matching barcode information— making import of sample names a one-click process. There is less opportunity for error, with an exact record of plates analyzed, and easier, faster operation. Plate map found with barcode matching position D. Promega Support – Now in Instrument Form Promega chemistry and hardware are integrated to provide optimized results for the Spectrum CE System. We offer on-site installation and training for the instrument, software and STR kits, as...
Open the catalog to page 12Easy-to-use Instrument Control and Data Software System Software The Spectrum Control Software provides a simple user interface with a clear display of useful features including run scheduling, consumables and array usage information, as well as system maintenance reminders. Analysis Software The Spectrum CE System creates both .fsa and .promega output files for sample analysis. The instrument’s .fsa output for existing 4-, 5- or 6-color multiplexes is compatible with GeneMapper® ID-X and GeneMarker®HID-Spectrum software analysis packages. Labs can generate .promega output for 4-, 5- or...
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