Solid Phase Extraction & Protein Crash
8Pages

LIFE SCIENCES CUSTOM MANUFACTURE AUTOMATION ACCESSORIES EQUIPMENT STORAGE/COLLECTION 36 S O L I D P H A S E E X T R A C T I O N œ Choice of popular sorbents œ Packed bed volumes from 10mg to 100mg per well œ Frit pore size 20ìm œ No channelling due to proprietary sorbent loading technique œ Working volume of 2ml per well œ Up to four times quicker than cartridge systems œ Long drain directors locate accurately with collection plate to avoid cross contamination œ Virgin polypropylene, tested and chosen for having no extractables œ Plate designed to be automated, meets SBS/ANSI specification MicroLute™ Solid-phase extraction (SPE) is a method of sample preparation that concentrates and purifies analytes from solution by sorption, followed by elution of the analyte with a solvent appropriate for instrumental analysis, such as LC-MS. Porvair Sciences offers a complete range of products to implement SPE. MicroLute™ is the original 96-well plate format for SPE. This device consists of a polypropylene 96-well plate loaded with a choice of sorbent and sorbent volumes. The frits have a mean pore size of 20 microns and are made from microporous high-density polyethylene. W W W . P O R V A I R - S C I E N C E S . C O M

Combinatorial MicroLute™ Description Qty/pack Cat. no. MicroLute™ plate, unpacked with bottom frits, Mean pore size 36ìm 1 240002 Mean pore size 36ìm, bag of 1000 frits, polyethylene 1 239007 MicroLute™ – accessories Description Qty/pack Cat. no. Disposable reservoir tray 25 219010 Sealing cap, square well, fits to top of plate 50 219004 Drain cap mat, seals base of plate 25 219005 Reservoir tray, polypropylene 25 390001 œ Disposable reservoir tray. Used for the collection of waste products in the vacuum manifold œ Sealing cap, square well, fits top of MicroLute™. Used for retaining the...

LIFE SCIENCES CUSTOM MANUFACTURE AUTOMATION ACCESSORIES EQUIPMENT STORAGE/COLLECTION S O L I D P H A S E E X T R A C T I O N W W W . P O R V A I R - S C I E N C E S . C O M SPE starter pack 39 This kit has been designed to form the starting point for SPE analysis. This gives the researcher the necessary hardware to process samples using an SPE plate and to collect under vacuum the analyte for further analysis. MicroLute™ starter pack Starter pack 271023 Development MicroLute™ This is an SPE Development MicroLute™, which is designed for research and development laboratories, where it may not...

S O L I D P H A S E E X T R A C T I O N MicroLute™ BioVyo Co-sinter products BioVyon™ Co-sinter products are made using a solid-state media created from an ultra-pure and highly modified polymeric material with the lowest levels of extractables and leachables. Our proprietary BioVyon™ technology allows high-purity silica resins, and immuno-capture agents such as Protein A, to be supported in a matrix which provides a high surface area whilst reducing channelling through the column. As the porous material is co-sintered under high pressure and temperature with the BioVyon™ polymer matrix,...

LIFE SCIENCES CUSTOM MANUFACTURE AUTOMATION ACCESSORIES EQUIPMENT STORAGE/COLLECTION 41 œ Solid-state media. No slurries to re-suspend, equilibrate or pour œ Ready to be used the minute you need them. œ Eliminates channelling, even at fast flow rates œ Can be rapidly regenerated, without re-packing œ Plates & columns can be re-used several times œ Cuts the cost of antibody purification. Protein A products BioVyon™ Protein A products are a new, ultra-fast method for antibody purification. Protein A from Staphylococcus binds especially the IgG class of immunoglobulins. BioVyon™ Protein A...

S O L I D P H A S E E X T R A C T I O N W W W . P O R V A I R - S C I E N C E S . C O M LIFE SCIENCES CUSTOM MANUFACTURE AUTOMATION ACCESSORIES EQUIPMENT STORAGE/COLLECTION 42 œ Unique design eliminates mess associated with traditional manual SPE cartridge methods œ Up to 4 times faster than cartridge methods œ Constructed from a single piece of high quality polymer which will not bend or distort œ A range of packed bed volumes ranging from 250mg, through to 1500mg œ Capability to process up to 200ml of sample in one plate œ Frit pore size of 20ìm œ Each well has an individual drain spout...

Biological samples commonly contain proteins that interfere with downstream applications. The P3 plate uses the CRASH method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time. The Protein Precipitation Plate, P3, is based on the industry standard MicroLute™ format, but without the chromatographic sorbent. Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up. The novel dual...