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Sample preparation

Sample preparation

Sample preparation

Product catalog summary
Introduction to Solid Phase Extraction (SPE):
Solid Phase Extraction (SPE) is a sample preparation method used to concentrate and purify analytes from a solution. This is achieved through sorption followed by elution with a suitable solvent for instrumental analysis, such as LC-MS. Porvair Sciences offers a range of products to facilitate SPE, including the MicroLute™ and MaxiLute™ systems.
MicroLute™ System:
The MicroLute™ is a 96-well plate format for SPE, made from polypropylene and available with various sorbents and volumes. Key features include a frit pore size of 20µm, a working volume of 2ml per well, and compatibility with automation systems. The system is designed to be up to four times faster than traditional cartridge systems and is manufactured to ANSI/SBS standards.
Combinatorial MicroLute™:
This system is based on the MicroLute™ format and allows for packing with any material required for chemical synthesis. It offers flexibility with two bottom frit sizes (10µm and 30µm) and is suitable for research and development applications where specific sorbents are needed.
MaxiLute™ System:
The MaxiLute™ is a 48-well SPE system designed for larger sample volumes, offering advantages for drug development and environmental laboratories. It features a range of packed bed volumes from 250mg to 1500mg and can process up to 200ml of sample per plate.
P3 Protein Precipitation Plates:
The P3 plate uses the CRASH method to denature proteins with acetonitrile, allowing for efficient protein precipitation and clean-up. It features a dual frit design to trap large and fine protein particles and is compatible with automation systems.
MicroLute™ Accessories and Starter Packs:
Porvair Sciences provides various accessories for the MicroLute™ system, including disposable reservoir trays, sealing caps, and drain cap mats. Starter packs are available for SPE analysis, providing the necessary hardware and sorbents for sample processing.
BioVyon™ Co-sinter Products:
BioVyon™ Co-sinter products are made from a highly modified polymeric material with low extractables and leachables. These products support high-purity silica resins in a matrix that reduces channelling and allows for high flow rates.
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Catalog excerpts

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SOLID PHASE EXTRACTION LIFE SCIENCES Solid phase extraction (SPE) is a method of sample preparation that concentrates and purifies analytes from solution by sorption, followed by elution of the analyte with a solvent appropriate for instrumental analysis, such as LC-MS. Porvair Sciences offers a complete range of products to implement SPE. MicroLute™ is the original 96-well plate format for SPE. This device consists of a polypropylene 96-well plate loaded with a choice of sorbent and sorbent volumes. The frits have a mean pore size of 20 microns and are made from microporous high-density polyethylene....

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TISSUE CULTURE & EPIGENETICS AUTOMATION ACCESSORIES LIFE SCIENCES SOLID PHASE EXTRACTION MicroLute™ packed with Varian sorbent Description C18 C8 C2 C1 PH CH CN-E C18-OH CN-N 20H Si CBA PRS SCX NH2 PSA DEA SAX PBA ENV LMS PPL C18HF Certify Certify HF Certify II Certify II HF Combinatorial MicroLute™ Combinatorial MicroLute™ is based on the tried and tested MicroLute™ format. This is a 96-well polypropylene plate with a bottom polyethylene frit already in place. It is designed to allow packing with any material required for chemical synthesis. Two bottom frit sizes are available, 10µm and 30µm,...

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SOLID PHASE EXTRACTION MaxiLute™ The development of a 48-well solid-phase extraction (SPE) system allows the step between SPE cartridges and SPE 96-well plates to be managed. LIFE SCIENCES With the larger packed bed volume and increased sample volume, the design of the MaxiLute™ offers advantages for drug development, food and environmental laboratories currently using SPE cartridges. Manufactured from virgin polypropylene and loaded with a choice of sorbents and volumes. Up to 4 times faster than cartridge methods Constructed from a single piece of high quality polymer which will not bend or...

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LIFE SCIENCES SOLID PHASE EXTRACTION P3 Protein Precipitation Plates Biological samples commonly contain proteins that interfere with downstream applications. The P3 plate uses the CRASH method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time. The Protein Precipitation Plate, P3, is based on the industry standard MicroLute™ format, but without the chromatographic sorbent. Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated...

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Development MicroLute™ This kit has been designed to form the starting point for SPE analysis. This gives the researcher the necessary hardware to process samples using an SPE plate and to collect under vacuum the analyte for further analysis. This is an SPE Development MicroLute™, which is designed for research and development laboratories, where it may not be known which sorbent best suits the process. The system allows the researcher to specify which sorbents go into specific wells, with what designated packed bed volume. This allows the researcher to have full control of the SPE plate. 2...

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LIFE SCIENCES SOLID PHASE EXTRACTION MicroLute™ BioVyo Co-sinter products BioVyon™ Co-sinter products are made using a solid-state media created from an ultra-pure and highly modified polymeric material with the lowest levels of extractables and leachables. Our proprietary BioVyon™ technology allows high-purity silica resins to be supported in a matrix which provides a high surface area whilst reducing channelling through the column. As the porous material is co-sintered under high pressure and temperature with the BioVyon™ polymer matrix, the resultant frit or column is impervious to further...

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P/N 420001 © Porvair Sciences Limited 2012. Produced by Cream Ink Limited www.creamink.com Printed in the UK. Accounts Porvair plc 7 Regis Place Bergen Way Kings Lynn Norfolk UK PE30 2JW Tel: 44 (0)1553 765500 Fax: +44 (0)1553 765599 Customer Services Porvair Sciences Ltd Clywedog Road South Wrexham Industrial Estate Wrexham North Wales UK LL13 9XS Tel: +44 (0) 1978 666240/666239 Fax: +44 (0) 1978 660007 Sales Office Porvair Sciences Ltd Dorset House Regent Park Kingston Road Leatherhead UK KT22 7PL Tel: +44 (0)1372 824290 Fax: +44 (0)1372 824271 email: [email protected] www.microplates.com...

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