STORAGE/COLLECTION FOREWORD Welcome to the 2012 edition of the Porvair Sciences Microplate Catalogue. Many scientists around the world have come to rely upon the Porvair catalogue as a primary source of information that enables them to choose the right microplate or tissue culture consumable. LIFE SCIENCES This revised edition for 2012 brings together information on Porvair Sciences comprehensive microplate and microplate instruments product range, together with our newly introduced Epigenetics & Tissue Culture Plastics range. Reflecting our commitment to continuous microplate technology innovation,...

CONTENTS Contents Application table Page 4 Storage/Collection 96-well deep square 96-well deep round 96-well shallow round 96-well round low profile 96-well glass vial storage plate 384-well Large volume Page 6 Page 7 Page 8 Page 9 Page 9 Page 10 Page 11 Life Sciences PCR plates – polypropylene Choosing the correct assay plate Solid bottom assay plates Clear bottom assay plates Glass bottom assay plates UV clear bottom assay plates Filtration plates Bacti-growth plates Components for assay development Page 13 Page 18 Page 20 Page 24 Page 28 Page 30 Page 31 Page 35 Page 36 Solid phase extraction...

A P P L I C AT I O N TA B L E Application table CL EN CO UN SC ER ■ Y ■ ■ ■ E ■ ■ AG ■ G ■ S ■ OV OR IC IS IN M EN EO YS AL RE ST DI D SC OT AN G IN G IN ■ ■ ■ UG PO S PR L ST EN TE RE S Y GY IE TR LO IS UD NO ST EM CH SC OD TA S/ HT IC EN E TE FO PL DR M M NM NO RO M Y TH CH ■ GE VI SA RA AL OW RI GR TO AR NA AL BI IC M IN CO Storage/collection ■ Life science ■ Solid bottom ■ ■ Glass bottom ■ ■ ■ ■ ■ Quartz bottom ■ ■ ■ ■ ■ Clear bottom ■ PCR plates ■ ■ ■ ■ Filtration plates ■ ■ Bacti-growth plates SPE MicroLute ■ ■ MaxiLute CombiLute ■ ■ P3 ■ Equipment Manifolds ■ ■ ■ ■ Evaporators ■ ■ ■ ■...

STORAGE/COLLECTION Porvair 800000 Ramp 30°C/min Initial temp 70°C Hold 2 min Final temp 310°C Hold 5 min 700000 600000 Abundance 500000 400000 300000 200000 14.8 14.5 14.1 13.8 13.5 13.2 12 12.9 12.6 12.3 11 11.6 11.3 10.7 10.4 10.1 9.77 9.46 9.15 7.9 8.84 8.52 8.21 7.59 7.27 6.96 6.65 5.4 6.34 6.02 5.09 0 5.71 100000 Time Plate B 800000 700000 600000 500000 400000 300000 200000 100000 Time 14.5 14.1 13.8 13.5 13.2 12.9 12 12.6 12.3 11.6 11.3 11 10.7 10.4 10.1 9.77 9.46 9.15 8.84 8.52 8.21 7.9 7.59 7.27 6.96 6.65 6.34 6.02 5.4 5.71 0 5.09 Abundance Results from the GC-MS showed good performance...

STO R A G E / C O L L E C T I O N – 9 6 -W E L L D E E P S Q UA R E 96-well deep square 6 44mm height LIFE SCIENCES The family of 96 square well plates was designed so that it would make the interchange of plates simpler in automated systems. All three plates have the same geometry and the only variable is the well depth. ● Tested for low extractables Tight specification levels ● V bottom to allow total liquid removal, partial collection and to aid re-suspension ● No inner edges to allow better collection of magnetic beads ● Manufactured under DNase/ RNase free environment ● Working volumes of...

The plates are made in virgin polypropylene to minimise extractables. Working volume is 1 ml per well and total volume is 1.1 ml. Coloured plates aid identification when retrieving from storage. Black plates are suitable for storing light sensitive compounds. Manufactured from pre-tested Alphanumeric grid-referencing DNase/ RNase free Packed in sealed sleeves of 5 plates Rimmed version to stop cross contamination and enable a better Non rimmed version to allow insertion of vials, or where the seal Cylindrical well with round bottom for optimal mixing and recovery Very easy to use with automated...

STO R A G E / C O L L E C T I O N – 9 6 -W E L L S H A L LOW RO U N D 96-well shallow round SOLID PHASE EXTRACTION LIFE SCIENCES 8 14.7mm height Porvair Sciences has developed a number of storage/collection plates to help in the fields of cell biology, molecular biology, drug discovery, combinatorial chemistry, screening and genomics. The plates are manufactured under clean room conditions and a significant number are DNase/RNase free. They are mainly made from polypropylene, an inert material giving heat and solvent resistant qualities. Porvair Sciences offers the largest combination of well...

STO R A G E / C O L L E C T I O N – 9 6 -W E L L RO U N D LOW P RO F I L E 96-well round low profile 9 Allows stacking in higher densities ● Minimises space needed for compound storage ● Pure virgin polypropylene ● Matching snug-fitting cap mat Dimensioned drawing available on request. 96-well round low profile Description 96-well round low profile plate Working well vol Sterile Qty/case Cat. no. 1ml – 50 219250 EQUIPMENT 96-well glass vial storage plate 44mm height Chemically resistant ● No trace impurities for UHPLC ● Prevent evaporation with matching cap mat TISSUE CULTURE & EPIGENETICS Store...

Porvair Sciences has a selection of 384-well polypropylene plates to suit most applications. All of them are made from pre-tested polypropylene in Class 100000 clean rooms. • Manufactured for high density sample collection I storage working volume • Each are designed to allow almost total removal of liquid sample • Extra flat - allows plate sealing • Storage temperature down to -80°C • Available sterile and non sterile nnnnnnnnnn nnnnnn nnnnnnnnnn innnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnn nnnnnnnnnn nnnnnn Well shape, top & bottom

Large volume High volume deep well microplates Well shape, bottom shape No of wells Rectangle, V-bottom (bulk pack) 24 Rectangle, V-bottom (single pack) 24 Rectangle, V-bottom (with bar code) 24 The range of large volume plates is designed to meet special requirements. Focused on the combinatorial, environmental and food technology markets, it allows large volumes of samples to be transported in recognised ANSI/SBS format microplates, to allow greater automation. Plates will accept the Porvair universal lid (see page 55). All plates are manufactured from virgin polypropylene, can be heat sealed...