ASSAY PLATES

An introduction to clear bottomed assay microplates Porvair Krystal series clear bottom plates are now recognised We supply our Krystal clear plates treated for cell culture as industry-leading tools for Pharma drug discovery and with a low pressure gas plasma that produces an even screening. Our standard clear polystyrene bottom plates surface treatment right across the plate. It is particularly have bases just 0.4mm thick and are moulded from highly suited to cell lines which do not grow easily on plastics. Our polished tools to reduce flow lines, artefacts and scratches, plastic-bottomed plates...

Choosing the correct assay plate Choosing the correct microplate for your application can mean the difference between indifferent and great results. There are three basic methods of obtaining useful optical data from microplate-based samples. The simplest method is absorbance measurement. Where greater sensitivity is required, fluorescence measurements are preferred. Sensitivity can be ten times greater than simple absorbance measurements. The third method involves luminescence, a naturally occurring phenomenon exhibited by certain animal and plant species which can emit light. This is a biological...

96-well clear plates For routine adsorption, absorption, ELISA, mixing and storage applications the standard range of 96-well assay plates offers the perfect mix of affordability and high quality. Manufactured from high quality crystal polystyrene Flat bottom for spectrophotometric work V-bottom minimizing residual liquid Round (U) bottom for cell/particulate collection Robot compatible Working volumes from 275µl down to 10µl Solid assay plates Plate description 96 wells of 350µl with a flat bottom 96 wells of 270µl with a round bottom 96 wells of 220µl with a V bottom 96 wells of 350uL with...

96-well black, white & black with white wells Highly polished well to give better readings Specially designed plate featuring a white well set in a black matrix to overcome the problem of a very high luminescence causing false positives Complies with the standard ANSI/SLAS format The acclaimed composite black/white plate has been shown to be ten times more sensitive than a standard white plate for chemi-luminesence assays. White plates maximise signal for luminescence readers These plates are designed to give optimum results from any instrument which reads from the top of the plate. Black plates...

384-well plates 384-well assay plates from Porvair Sciences are available in black, white and clear. These have been optimised for fluorescence, luminescence/scintillation and ELISA/turbidity measurements respectively. There are two working volumes: 30µl/well or 120µl/well. All 384-well assay plates are available in standard, high bind and tissue culture treated formats. NEW Very low volume 5µl clear TC plate 500079 Round well low volume Square well plates The low volume 384-well assay plate is designed for high throughput screening with 30µl total volume to reduce the amount of sample and reagent...

Solid assay plates Sterile High bind with lid Tissue culture Tissue culture Hi protein bind with lid Tissue culture Tissue culture Hi protein bind with lid Tissue culture Tissue culture ROUND WELL Plate description Tissue culture Tissue culture Tissue culture Tissue culture

24-well Krystal™ black & white Clear bottom plates allow the cells grown on the bottom surface to be viewed using an inverse microscope. In addition, light-emitting assays can be measured from the bottom. Porvair Sciences collection of clear bottom 24well Krystal plates are optimised for fluorescence and luminescence. The special design of the plate virtually eliminates wellto-well light crosstalk inherent with other clear-bottomed microplate designs, enabling unmatched accuracy, higher sensitivity and better repeatability of photometric readings. For photometric applications where samples are...

96-well Krystal™ black and white Porvair Sciences clear bottom 96-well Krystal plates are optimised for fluorescence and luminescence. Proprietry ultra-sonic welding is used to assemble the clear bases to the solid top plate in our clean room. This design provides flat, optically clear bases to each well with no leakage, enabling excellent accuracy and reproducibility of photometric readings. All tissue-culture treated plates are supplied lidded and sterile, in individual bags. Opaque walls to prevent well-towell crosstalk Clear plate bottom permits direct microscopic viewing For use with top...

384-well Krystal™ black and white The Krystal 384-well microplate range incorporates novel square wells with a large liquid capacity of 120µl per well, increasing the achievable sensitivity of most HTS assays. Using a proprietary manufacturing technique, the Krystal 384 offers market leading plate flatness (+/- 0.1mm tolerance) that translates into a significant increase in measurement precision and elimination of read errors when performing cell based assays using fluorescent or luminescent imaging. All tissue-culture treated plates are supplied lidded and sterile, in individual bags. Opaque...

96-well Krystal™ 2000 black and white Lowered bottom rim to stop lateral light piping Clear well bottom permits direct microscopic viewing For use with top or bottom reading instruments Designed to the standard ANSI/SLAS format Total well volume of 350µl White matrix provides for maximum reflectivity, allowing high sensitivity luminescence assays Black matrix provides a quenching of background fluorescence, reducing false positives The unique Krystal 2000 plate range from Porvair Sciences has been optimised for luminescence and fluorescence assays. A patented manufacturing process, using a ‘two-shot’...

Krystal™ glass bottom plates Manufactured for whole-plate CCD imaging and laser detection applications, Krystal glass bottom plates consist of a polystyrene upper part and a clear borosilicate glass sheet fixed to the base with a proprietary adhesive. This process results in consistent flatness of the base and gives improved light transmission whilst maintaining a flat optical plane for growing cells. The nominal cut-off wavelength of 335nm allows most fluorescence assays to be excited or read through the glass bottom. All plates are supplied lidded. +/- 15µm flatness across base Industry-standard...