TriTom
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Discover the Power of Light and Sound Multimodal whole body in-vivo Imaging Platform Co-registered photoacoustic/ fluorescence 3D tomography Molecular & functional imaging with high-resolution anatomical registration Optimized for small murine research models

Co-registered Multimodality 3D Imaging True 3D anatomy with sub-millimeter spatial resolution: 160 µm x 160 µm in transverse planes • 160 µm x 470 µm in sagittal and coronal planes • TriTom composite 890 nm + 532 nm imaging of a life mouse vasculature Emission filters Rotary scanning mechanism Laser excitation ports Enhance imaging benefits, eliminate shortcomings • TriTomTM utilizes simultaneous spectrallyselective optical and photoacoustic excitation and detection to create unparallel volumetric assessment of live organisms, organs, and tissues. sCMOS camera Photoacoustic transducer array...

TriTom Applications Triple Analysis– anatomical, functional, and molecular Cancer Research Composite skin & deep tissue 3D image. 532 nm skin excitation. 890 nm deep tissue excitation. Tumor size 10.6 x 4.7 x 11.6 mm3. 7 mm MIP axial slab of mouse’s brain near the cerebellum/medulla. (1) confluence of sinus and (2) cerebral artery are marked

Functional Imaging Lower abdomen of a healthy mouse. Red – deep blood vessels, 890 nm excitation scan. Yellow – superficial vasculature (skin), 532 nm excitation scan. 1 – right subiliac lymph node. 2 – injection site (right mammary fat pad) Stem Cell Research 3D photoacoustic image of the spinal cord with injected cells denoted by arrows. Developmental Biology Pregnant mouse, gestational day 12 shows a composite skin / deep tissue photoacoustic image. Anatomical Details of Internal Organs PAT maximum intensity projection of mouse liver. (1) left lobe of liver; (2) right lobe of liver; (3)...

Development of Contrast Agents Crossectional (axial) views of 0.8 mm inner diameter of the microcuvettes with IRDye800CW samples. Accessories Mouse holder (in vivo imaging) Cylindrical cuvette holder (development of contrast agents) 1 High throughput: Interrogate up to 10 samples per scan  Preparation time < 5 min Reliable gas anesthesia Delivered through the hollow shaft Convenient & repeatable procedure:  Mouse’s front and hind legs and the head are fixed in a consistent position with minimal stress  Preparation time < 5 min • Save your valuable samples!  Tiny 50 µL sample 3 volumes •...

Patent Protected Technology Perfect co-registration of imaging modalities Both PA & FL are initiated simultaneously using pulsed high-efficiency laser light Both PA & FL are enabled for molecular imaging by the tuning excitation wavelength during the scan. Tuning is necessary for multi-spectral imaging. Excitation Source Excitation source: Tunable OPO laser Wavelength tuning range covering all popular visible, NIR I and NIR II fluorophores and nanoparticles:  460 – 659 nm (extended visible excitation) Up to 250 mJ peak energy @ 700 nm 10 Hz or 20 Hz pulse repetition frequency Excitation...

Software The TriTomTM imaging software suite provides a streamlined experience from simultaneous acquisition of optical and photoacoustic data to final reconstruction and co-registration of volumes. • Iconographic design of the main control screen Modular user-centric graphic Interface Optical frames Scan metadata Open data formats TriTom image & signal monitoring interface 3D slicer — visualization and image processing

TriTom PhotoAcoustic Edition PhotoAcoustic (PA) Imaging Channel Type Spatial resolution Molecular imaging sensitivity PA excitation range Detection points per scan Detector configuration Detector central frequency Detector bandwidth @ -6 dB Number of array elements Detector working environment PA signal digitizer High-resolution deep tissue molecular, physiological, and anatomical imaging, subcutaneous & skin imaging Transverse anatomical planes Sagittal and coronal anatomical planes In blood plasma, multispecies molecular unmixing, CNR 1.7 Extension to 2300 nm with an optional OPO idler...

TriTom PhotoAcoustic Edition Laser Excitation Unit Option: Vis-NIR II, dual-range FWS (650 - 1300 nm & 1065 2300 nm) Optional upgrade to 20 Hz For a 20 Hz PRF option, max pulse energy 160 mJ @ 700 nm Tunable wavelength range Pulse repetition frequency 10 (20) Hz > 180 mJ @ 700 nm > 20 mJ @ 532 nm Real-time automatic pulse energy measurements Change to any wavelength between 650 - 1300 nm every 100 (50) ms Rolled on wheels, positioned on the floor next to the Image Mobile Acquisition Unit 68 cm x 44 cm x 89 cm 208 or 240 VAC, single phase 50/60 Hz, < 1.5 kVA Pulse Energy Energy meter Fast...

TriTom Standard Edition PhotoAcoustic (PA) Imaging Channel Type Spatial resolution Molecular imaging sensitivity PA excitation range Detection points per scan Detector configuration Detector central frequency Detector bandwidth @ -6 dB Number of array elements Detector working environment PA signal digitizer Fluorescence (FL) Imaging Channel Type Spatial resolution FL excitation range Excitation linewidth Emission filter set Optical filter wheel Detector type Bit depth Number of pixels Pixel resolution Max frame rate Dynamic range Quantum efficiency Readout noise Dark current...

TriTom Standard Edition Image Acquisition Unit Single scan time Scan types Excitation sequence Max volume of a 3D image Whole body imaging In vivo imaging subjects Max weight of the test subject Coupling liquid Environment temperature control Test subject monitoring Laser safety Chassis type Dimensions (L x W x H) Power requirements 36 s 360 deg azimuthal rotation, 360 (720) data frames Continuous azimuthal rotation or reverse scans ( 360 deg), time-limited by 10 min Single wavelength; Linear or custom wavelength sweep; Popular spectral unmixing presets for molecular, physiological and...

TriTom Premium Edition PhotoAcoustic (PA) Imaging Channel Type Spatial resolution Molecular imaging sensitivity PA excitation range Detection points per scan Detector configuration Detector central frequency Detector bandwidth @ -6 dB Number of array elements Detector working environment PA signal digitizer High-resolution deep tissue molecular, physiological, and anatomical imaging, subcutaneous & skin imaging 160 µm x 160 µm Transverse anatomical planes 160 µm x 470 µm Sagittal and coronal anatomical planes 100 nM ICG In blood plasma, multispecies molecular unmixing, CNR 1.7 460 - 1300...