Photometrics XT2™ Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Collimated Emission-Port Adapter For many imaging applications, it is necessary to utilize devices such as filter wheels and LCTFs on the emission port of a microscope. The beam emerging from the microscope emission port, however, is coming to a focus. Optical devices are often placed in the focusing beam, thereby producing a series of errors, including: Correct emission imaging with the XT2! Filter in focusing beam Filter in focusing beam Parfocality problem! Anytime a flat piece of glass (e.g., an emission/barrier filter) is inserted Focusing light in a microscope beam, the beam will no longer focus at its original from focusing location; instead, the location of the focal plane will be shifted. The amount of shift will depend on the thickness of the filter as well as the index of refraction of the glass used to make the filter. This focal-shift New focal plane error destroys parfocality between the detector and the eyepiece. Original focal plane However, when collimated light passes through an emission filter, the focal plane is not shifted. Bandpass Variation Parfocality problem! Filter in focusing beam Focusing light from microscope Parfocality problem! Focusing light from microscope New focal plane Original focal plane New focal plane Original focal plane Filter in focusing light Filter in focusing light It is a well-known fact that the bandpass of a filter varies with the angle at which light hits it. When a filter is placed in a focusing beam, Edge ray the light at the edge of the beam wavelengthfilter at a different angle than λ2 Center hits the λ1 theCenter ray the center of the(CWL) of bandpass light at beam. As a result, there is a variation in is different at edge λ2 the center wavelength (CWL) of the filter bandpass for different parts and center! Edge ray of the beam. However, when collimated light passes through a filter, all rays strike at the same angle, eliminating bandpass shift. Filter in focusing light Edge ray Center ray Edge ray Edge ray ray Center Center wavelength (CWL) of bandpass is different at edge and center! Center wavelength (CWL) of bandpass is different at edge and center! Edge ray → Angle of incidence changes from edge to center. → Angle of incidence changes from edge to center. → Angle of incidence changes from edge to center. The Solution… Filter in collimated light No wavelength shift! All light hits filter at same angle. λ1 λ1 λ1 Focusing light from microscope PM-DS-017 Rev B3 ©2013 Photometrics. All rights reserved. No focal shift! Original focal plane is preserved. • Two-piece optical adapter creates 1 – 4” of collimated (infinity) Filter in collimated light space at emission port of microscope, thus allowing addition of wavelength shift! No All light hits filter other optical devices without sacrificing performance or Filter in collimated light at same angle. No wavelength shift! introducing optical aberrations Focusing light from microscope • Provides “extra space” at microscope emission Focusing light port for installation of instrumentation such as filter from microscope wheels and liquid-crystal tunable filters (LCTFs) Lens systems in XT2 • Flexible enough for use with a variety of optical Lens systems devices with C-mounts in XT2 λ1 All light hits filter λ1 at same angle. λ1 λ1 λ1 λ1 No focal shift! Original focal plane is preserved. No focal shift! Original focal plane is preserved.

Collimated Emission-Port Adapter XT2 Specifications Detector options Attachment thread Max diagonal dimension1 Microscope attachment options C-mount (female) Device attachment options C-mount (male and female) Wavelength sensitivity Operating temperature Specifications may change without notice. 1. without experiencing measurable image distortion 2. assumes 100x, 1.6 NA microscope objective 1 – 4" Optical devices (e.g., filter wheel or LCTF) Anodized aluminum finish on all parts C-mount male or female thread: attaches to device C-mount male or female thread: attaches to device C-mount female...