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QV2 Simultaneous Acquisition of up to Four Emission Channels

QV2 Simultaneous Acquisition of up to Four Emission Channels

QV2 Simultaneous Acquisition of up to Four Emission Channels

Product catalog summary
Overview: The Photometrics® QV2™ is a four-channel simultaneous-imaging system designed for life sciences applications. It allows for the simultaneous acquisition of up to four emission channels in a single exposure, which is essential for quantitative analysis of emission ratiometric data.
Features:
  • Simultaneous acquisition of up to four images, separated by wavelength, polarization, or amplitude.
  • Compatible with most microscopes and offers improved adjustment control for easier image alignment.
  • Redesigned aperture adjustments ensure parallel apertures.
  • Uses standard 25-mm-diameter emission and polarization filters.
  • Bypass mode with a bypass filter cube for full-field imaging without hassle.
  • Exchangeable filter cube for multiple applications with minimal realignment.
  • Integrated, adjustable CCD mask minimizes ghosting.
  • Compatible with many Photometrics® and QImaging® cameras.
Specifications:
  • Wavelength sensitivity: 400 to 750 nm.
  • Efficiency per image: 70 to 92%.
  • Operation temperature: 10 to 37˚C.
  • Detector attachment: C-mount (male); Front attachment: C-mount (female).
  • External mounting option: ¼-20 tapped hole on the back of the unit.
  • Dimensions: 2.5” diameter x 7.5” height; Weight: 2.6 lbs.
  • Filters: Emission/barrier, neutral density, polarization; 1” max diameter; 7-mm max thickness.
  • Patents: USA: 5,926,283 and 5,982,497; Australia: 731,476; Canada: 2,294,840; Other foreign patents pending.
Applications:
  • Fluorescence resonance energy transfer (FRET) imaging.
  • Multicolor single-molecule fluorescence (SMF) imaging.
  • Multiwavelength total internal reflection fluorescence (TIRF) imaging.
  • Fluorescence in situ hybridization (FISH) imaging.
  • Multichannel confocal microscopy with a spinning-disk confocal.
  • Two-color polarization/anisotropy studies.
  • Simultaneous calcium and pH studies with indo-1 and SNARF.
  • Three-color fluorescence and DIC.
  • Polarized FRET analysis.
  • Simultaneous 3D imaging when lenses replace emission filters.
Contact Information: For more details, visit www.photometrics.com or contact via email at [email protected] or phone at +1 520.889.9933.
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Catalog excerpts

QV2 Simultaneous Acquisition of up to Four Emission Channels-1

Photometrics QV2™ Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Four-Channel Simultaneous-Imaging System The Photometrics® QV2™ allows simultaneous acquisition of up to four emission channels in a single exposure. The QV2 uses a series of beamsplitters to split the emission light from a microscope into four separate channels. All four channels are projected onto the CCD at the same time. Simultaneous multichannel imaging is critical for quantitative analysis of emission ratiometric data. Features • Simultaneous acquisition of up to four images • Images can be separated by wavelength, polarization, or amplitude Molecular Probes Alexa Fluor® 350 Molecular Probes SYTOX® Green Molecular Probes Alexa Fluor® 594 Color Overlay • Easily mounts to most microscopes • Improved adjustment control enables easier image alignment • Redesigned aperture adjustments ensure apertures are parallel • Uses standard 25-mm-diameter emission and polarization filters • Bypass mode with bypass filter cube permits no-hassle, full-field imaging • Exchangeable filter cube allows multiple applications to be run with minimal realignment • Integrated, adjustable CCD mask minimizes ghosting • Works with many Photometrics® and QImaging® cameras* *Please contact your local representative to verify compatibility with specific cameras. PM-DS-014 Rev B3 ©2013 Photometrics. All rights reserved.

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QV2 Simultaneous Acquisition of up to Four Emission Channels-2

Four-Channel, Simultaneous-Imaging System Wavelength sensitivity Operation temperature Detector attachment C-mount (male) • Multiwavelength total internal reflection fluorescence (TIRF) imaging Front attachment C-mount (female) • Fluorescence in situ hybridization (FISH) imaging ¼-20 tapped hole on back of unit • Multichannel confocal microscopy when used in conjunction with a spinning-disk confocal • Two-color polarization/anisotropy studies Emission/barrier, neutral density, polarization; 1” (25.4-mm) max diameter; 7-mm max thickness External mounting option • Fluorescence resonance energy...

 Open the catalog to page 2
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.