Prime sCMOS Camera Datasheet
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Prime™ sCMOS Camera Datasheet CMOS, EMCCD AND CCD CAMERAS FOR LIFE SCIENCES 4.2 Megapixel Scientific CMOS Camera Photometrics Prime is the first intelligent scientific CMOS (sCMOS) camera to incorporate a powerful FPGA-based Embedded Signal Processing™ engine (ESP). ESP enables advanced real-time processing features: PrimeEnhance™ quantitatively increases the Peak Signal to Noise Ratio by 3X-5X, increasing the clarity and quality of images. PrimeLocate™ dynamically evaluates acquired images and reduces the surplus of data generated during high speed super resolution imaging. Primary applications: Designed using sCMOS sensor technology, Prime becomes an extremely Super Resolution Microscopy versatile imaging solution for life science imaging. It is a high resolution camera with great sensitivity, extremely low noise, high frame rates and Light Sheet Microscopy an impressive dynamic range. The large field of view is ideal for High Speed Ratiometric Imaging microscopy, maximizing usefulness of the imaging area. TIRF Microscopy Confocal Microscopy Extremely Low Read Noise Maximize your ability to detect faint fluorescence Fast Frame Rates Capture highly dynamic events with fast temporal resolution Large Field of View Maximize the number of cells that can be tracked and monitored per frame Optimized Pixel Size Properly sample your microscope’s image at its full resolution High Quantum Efficiency Enables short exposure times to minimize phototoxicity and enable high frame rates across a wide wavelength spectrum Enhanced Dynamic Range Measure both bright and dim signal levels within the same image Improve image quality and imaging efficiency with FPGA based Embedded Signal Processing engine for real-time image processing PrimeEnhance PrimeLocate Multi-ROI (Region of Interest) Multiple Expose Out Triggering Real-time quantitative denoising algorithm that improves image clarity by reducing photon-shot (Poisson) noise. Delivers an increase in Peak Signal to Noise Ratio of 3X to 5X Dynamically evaluates and acquires only the relevant data for localization based super resolution applications Acquire only regions of data with desired information, and reduce data glut Define up to 15 unique regions of interest and transfer only this data to the host computer Use the camera to control up-to four light sources for multi-wavelength acquisitions ©2015 Photometrics. All rights reserved.

Uncompromised Imaging Prime™ sCMOS Camera Datasheet 4.2 Megapixel sCMOS Sensor 1e- read noise 72% peak QE 30,000e- full well 18.8mm diagonal Embedded Signal Processing PrimeEnhance PrimeLocate Multi-ROI Easily Mounted and Secured C-mount Two ¼”-20 mounting holes per side Dual Interface High Speed PCI-Express • 100fps USB 3.0 • 30fps Multiple Cooling Options Forced Air Cooling • -10ºC Cooling • Selectable Fan Speed Liquid Cooling • -25ºC Cooling • Leak-proof, quick-disconnect ports Advanced Triggering Capabilities Effective Global Shutter Up to four selectable expose-out lines ©2015...

Prime™ sCMOS Camera Datasheet Uncompromised Imaging Embedded Signal Processing (ESP™) Features PrimeEnhance Due to the incredibly low electronic noise of sCMOS sensors, the latest generation of scientific cameras have enabled imaging using only a few photons per pixel. Unfortunately, these minute signals are dominated by the natural Poisson variation in light levels preventing useful quantitation. PrimeEnhance uses a quantitative SNR enhancement algorithm used in Life Science imaging to reduce the impact of photon shot-noise present in acquired images, leading to an increase in Signal to...

Prime™ sCMOS Camera Datasheet Uncompromised Imaging Embedded Signal Processing (ESP™) Features PrimeLocate Localization based super resolution microscopy requires a sparsity of data to ensure proper localization of emitting molecules. Even with this sparsity, the full image frame is transferred to the host to be analyzed, creating a large amount data to be processed without adding useful information. PrimeLocate dynamically evaluates image data and locates 500 regions per frame containing single molecule data relevant for super resolution localization. Only these 500 regions are transferred...

Prime™ sCMOS Camera Datasheet Uncompromised Imaging Camera Performance Array Size Pixel Area Sensor Area Read Noise Full-Well Capacity Dynamic Range Readout Mode Rolling Shutter Effective Global Shutter Cooling Performance Sensor Temperature Dark Current Liquid Cooled Camera Interface Digital Interface Lens Interface Mounting Points 2 x ¼ 20” mounting points per side to prevent rotation Liquid Cooling Quick Disconnect Ports Triggering Mode Input Trigger Modes Trigger-First – Sequence triggered on first rising edge Edge – Each frame triggered on rising edge SMART Streaming – Fast iteration...

Uncompromised Imaging Prime™ sCMOS Camera Datasheet Array Size PCI-Express Interface Card PCI-Express Cable Trigger Cable Power Supply Manuals and QuickStart Guide Performance and Gain Calibration Test Data Liquid Circulator Liquid Cooling Tubes Distance from C-mount to sensor Photometries is a registered trademark Prime, PrimeEnhance, and PrimeLocate are trademarks of Photometries All other brand and product names are the trademarks of their respective owners Specifications in this datasheet are subject to change. Refer to the Photometries website for most current specifications. Rev...