QuantEM™ 512SC Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES 512 x 512 imaging array 16 x 16-μm pixels The Photometrics® QuantEM™ 512SC is the world’s first and only electron-multiplying CCD (EMCCD) camera to offer on-chip signal amplification with true quantitative stability across 16 bits at readout rates of 10, 5, and 1.25 MHz. The high-speed, highperformance QuantEM lets you conduct precision ratiometric analysis in time-course experiments, acquire reproducible data during long-term studies, and capture streaming data for multidimensional time-lapse investigations — all with single-molecule sensitivity. A patent-pending PAR™ feedback system provides exceptional stabilization of EM gain, while an intelligent FPGA design facilitates self-calibrating linearization of EM gain and prevents bias drift over time. Furthermore, patentpending ACE™ technology enables superior timing resolution of the device’s pixel clocks, allowing optimal signal-to-noise sampling and minimizing spurious charge. Primary applications Quantitative FRET Multiprobe experiments Ratiometric ion imaging Confocal microscopy Live-cell fluorescence imaging Very high sensitivity Low-noise, impact-ionization process Back-illuminated EMCCD Highest available quantum efficiency (>90% peak QE) 512 x 512 imaging array 16 x 16-μm pixels Good field of view and sensitivity Good resolution Intelligent FPGA design Precise linearization of EM gain Self-calibrating linearization ensures truly quantitative data all the time Prevents bias drift over time to guarantee a stable background PAR* feedback system (Photometrics Active Regulation) Delivers unsurpassed EM gain stability for outstanding signal fidelity across 16 bits ACE* technology (Advanced Clocking Enhancement) Pixel-clock timing resolution 12x better than competing EMCCD cameras Provides lowest noise floor and minimizes generation of spurious charge and background events Excellent for high-speed image visualization Perfect for high-precision photometry Dual amplifiers Select readout mode via software for optimized (1) high-speed / high-sensitivity performance or (2) wide-dynamic-range performance Wide dynamic range allows detection of bright and dim signals in the same image Frame-transfer EMCCD 100% duty cycle to collect continuous data No mechanical shutter required Easily attaches to microscopes, standard lenses, or optical equipment High-bandwidth, uninterrupted data transfer with no dropped frames Windows® XP/Vista 32 and Mac OS X compatibility PVCam® Circular buffers Device sequencing Supported by numerous third-party software packages Real-time focus Precise integration with shutters, filter wheels, etc. * Patent-pending Photometrics technology PM-DS-001 Rev C3 ©2012 Photometrics. All rights reserved.

Superior Quantitative EMCCD Imaging Quantum Efciency (%) Note: Frame rates are measured at 10 MHz with 0-second exposure times in expose mode ALT_ FT. EMCCD image sensor e2v CCD97; back-illuminated, frame-transfer CCD with EM gain EMCCD format 512 x 512 imaging pixels; 16 x 16-μm pixels; 8.2 x 8.2-mm imaging area (optically centered) Linear full well single pixel output node Digitizer type Dual-selectable 14 bits/16 bits for all speeds and gains 16-bit digitization minimizes quantification error, enabling extreme low-light detection “EM gain” amplifier (port #1) “Traditional” amplifier (port...