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Photometrics Multichannel Systems

Photometrics Multichannel Systems

Photometrics Multichannel Systems

Product catalog summary
Overview
The document provides detailed information on high-performance EMCCD and CCD cameras designed for life sciences applications, specifically focusing on multichannel imaging systems by Photometrics. These systems are essential for quantitative emission ratiometric imaging and are compatible with various microscopes and cameras.
Key Features
  • Simultaneous acquisition of multiple full-field emission images.
  • Emission separation by wavelength, polarization, or amplitude.
  • Increased scanning speeds for high-content screening applications.
  • Easy mounting and improved adjustment controls for image alignment.
  • Exchangeable filter cubes for versatile applications with minimal realignment.
  • Bypass mode for directing emission light to a single camera.
  • Integrated, adjustable CCD mask to minimize ghosting.
  • Independent focus adjustment for each camera.
Specifications
  • Wavelength sensitivity: 400 to 750 nm.
  • Efficiency per image: 70 to 92%.
  • Operation temperature: 10 to 37˚C.
  • Detector and front attachment: C-mount (male and female).
  • External mounting option: ¼-20 tapped hole on the back of the unit.
  • Dimensions and weight vary by model.
  • Patents held in the USA, Australia, and Canada, with other foreign patents pending.
Applications
  • Fluorescence resonance energy transfer (FRET) imaging.
  • Calcium imaging with fluo-3/Fura Red™ or dual-emission indo-1 imaging.
  • Fluorescence polarization/anisotropy imaging.
  • Simultaneous fluorescence/DIC imaging.
  • Drug discovery with Cy3/Cy5.
  • Single-molecule fluorescence (SMF) imaging.
  • pH imaging with SNARF.
  • Multiwavelength total internal reflection fluorescence (TIRF) imaging.
  • Voltage sensing with di-4-ANEPPS.
  • Fluorescence in situ hybridization (FISH) imaging.
  • cAMP imaging with FICRhR.
  • Multichannel confocal microscopy with spinning-disk confocal.
Models
  • DC2™: Dual-channel, full-field, simultaneous-imaging system.
  • DV2™: Utilizes a single-beamsplitter design for two independent channels.
  • QV2™: Allows simultaneous acquisition of up to four emission channels.
Conclusion
Photometrics multichannel systems offer advanced imaging capabilities essential for various life sciences applications, providing flexibility, efficiency, and compatibility with existing microscopy setups.
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Catalog excerpts

Photometrics Multichannel Systems-1

HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Multichannel Systems 3440 E. Britannia Drive Tucson, AZ 85706, USA Tel: +1 520.889.9933 www.photometrics.com [email protected]

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Photometrics Multichannel Systems-2

Photometrics DC2™ Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Dual-Channel, Full-Field, Simultaneous-Imaging System The Photometrics® DC2™ enables two-channel simultaneous imaging based on two distinct wavelengths or polarizations. Each channel is projected onto an individual CCD. Simultaneous multichannel imaging is essential to achieve quantitative emission ratiometric imaging. Features • Simultaneous acquisition of two full-field emission images Green Channel Red Channel • Emission can be separated by wavelength, polarization, or amplitude • Increases scanning speeds by...

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Photometrics Multichannel Systems-3

Photometrics DC2™ Datasheet Dual-Channel, Full-Field, Simultaneous-Imaging System DC2 Specifications Applications Wavelength sensitivity 400 to 750 nm Efficiency per image* 88 to 92% Operation temperature 10 to 37˚C Detector attachment C-mount (male) Front attachment C-mount (female) External mounting option ¼-20 tapped hole on back of unit Dimensions 6.5” at widest point x 8.5” height Weight 4.8 lbs Filters Emission/barrier, neutral density, polarization; 1” (25.4-mm) max diameter; 0.39” (9.9-mm) max thickness Patents • Fluorescence resonance energy transfer (FRET) imaging • Calcium imaging...

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Photometrics Multichannel Systems-4

Photometrics DV2™ Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Dual-Channel Simultaneous-Imaging System The Photometrics® DV2™ utilizes a single-beamsplitter design to split the emission light from a microscope into two independent channels. Each channel is projected onto half of the CCD at the same time. Simultaneous multichannel imaging is essential for FRET and other emission ratiometric applications. Features Green Red • Simultaneous acquisition of two emission channels • Emission can be separated by wavelength, polarization, or amplitude • Easily mounts to most microscopes...

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Photometrics Multichannel Systems-5

Photometrics DV2™ Datasheet Dual-Channel, Simultaneous-Imaging System DV2 Specifications Applications Wavelength sensitivity 400 to 750 nm Efficiency per image* 88 to 92% Operation temperature 10 to 37˚C Detector attachment C-mount (male) Front attachment C-mount (female) External mounting option ¼-20 tapped hole on back of unit • Fluorescence resonance energy transfer (FRET) imaging • Polarized FRET analysis Dimensions • Simultaneous fluorescence/DIC imaging • Drug discovery with Cy3/Cy5 4.8 lbs Filters • Fluorescence polarization/anisotropy imaging 6.5” at widest point x 8.5” height Weight...

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Photometrics Multichannel Systems-6

Photometrics QV2™ Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Four-Channel Simultaneous-Imaging System The Photometrics® QV2™ allows simultaneous acquisition of up to four emission channels in a single exposure. The QV2 uses a series of beamsplitters to split the emission light from a microscope into four separate channels. All four channels are projected onto the CCD at the same time. Simultaneous multichannel imaging is critical for quantitative analysis of emission ratiometric data. Features • Simultaneous acquisition of up to four images • Images can be separated by wavelength,...

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Photometrics Multichannel Systems-7

Photometrics QV2™ Datasheet Four-Channel, Simultaneous-Imaging System QV2 Specifications Applications Wavelength sensitivity 400 to 750 nm Efficiency per image* 70 to 92% Operation temperature 10 to 37˚C Detector attachment C-mount (male) • Multiwavelength total internal reflection fluorescence (TIRF) imaging Front attachment C-mount (female) • Fluorescence in situ hybridization (FISH) imaging ¼-20 tapped hole on back of unit • Multichannel confocal microscopy when used in conjunction with a spinning-disk confocal Dimensions 2.5” diameter x 7.5” height • Two-color polarization/anisotropy studies...

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Photometrics Multichannel Systems-8

HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES For more information on Photometries Multichannel Systems: 3440 E. Britannia Drive, Tucson, AZ 85706, USA www.photometrics.com [email protected] Copyright © 2012 Photometries. All rights reserved.

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.