Evolve™ 512 Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES 512 x 512 imaging array 16 x 16-μm pixels Smallest, most powerful scientific EMCCD camera on the market Most advanced feature set available for low-light applications Lowest dark current available for an EMCCD camera Lowest read noise available for an EMCCD camera Available with Exclusive Primary applications: Quantitative FRET Multiprobe experiments Technology Ratiometric ion imaging Confocal microscopy Live-cell fluorescence imaging Ideal for sophisticated researcher and multi-user labs Superb electron multiplication (EM) gain and bias stability Most accurate EM calibration technique in the industry Available with exclusive eXcelon™ technology Backed by Photometrics’ worldwide support team Features Benefits EM gain Very high sensitivity Low-noise, impact-ionization process Back-illuminated EMCCD CCD97 or CCD97-X (with eXcelon technology), highest available quantum efficiency (>90% peak QE) 512 x 512 imaging array 16 x 16-μm pixels Optimized field of view and highest sensitivity Intelligent FPGA design Precise linearization of EM gain Self-calibrating linearization ensures truly quantitative data consistently, time and again Ensures bias stability to guarantee a stable background PAR feedback system (Photometrics Active Regulation) Delivers unsurpassed EM gain stability for outstanding signal fidelity across 16 bits ACE technology (Advanced Clocking Enhancement) Pixel-clock timing resolution 12x better than other EMCCD cameras Provides lowest noise floor and minimizes generation of spurious charge and background events Excellent for high-speed image visualization Perfect for high-precision photometry Dual amplifiers Select readout mode via software for optimized (1) high-speed / high-sensitivity performance (EM) or (2) wide-dynamic-range performance (traditional CCD mode) Wide dynamic range allows detection of bright and dim signals in the same image Frame-transfer EMCCD 100% duty cycle to collect continuous data No mechanical shutter required Easily attaches to microscopes, standard lenses, or optical equipment Universally accepted interface that provides high-bandwidth, uninterrupted data transfer with no dropped frames Windows® XP/7 PVCam® Circular buffers Device sequencing Supported by numerous third-party software packages Real-time focus Precise integration with shutters, filter wheels, etc. Exclusive eXcelon Technology (optional) Enhanced QE in Blue and near IR wavelengths Anti-Etaloning in near IR wavelengths * Patent-pending Photometrics technology PM-DS-013 Rev A10 ©2013 Photometrics. All rights reserved.

Superior Quantitative EMCCD Imaging Photometrics Advanced Features Features Allows camera to read out pixel values in terms of electrons measured, thereby enabling user to calculate actual photon flux Electrons-per-gray-level selector Allows user to select how many electrons will cause a single gray-level increase in the image data, thus permitting utilization of Quant-View while maintaining full dynamic range of EMCCD 3-minute EM calibration process is most accurate in industry; camera does not need to be detached from microscope; no special attachments required; user-initiated Background Event...

Superior Quantitative EMCCD Imaging Bias Stability 10MHz Gain 3 350X Read Noise vs. Slider Value Note: Actual data, not theoretical. Multiplication Gain vs. Slider Value Actual Multiplication Gain (X) Note: Actual data. Frame Number Note: Frame rates are measured at 10 MHz with 0-second exposure times. Note: Actual data from camera. Exclusive eXcelon Technology (optional) 600 nm Standard Back-Illuminated EMCCD Quantum Efciency Curve 100% 90% 80% Reduced Etaloning Up to 10 times lower etaloning in near IR wavelengths compared to standard backilluminated sensors Improved Sensitivity Provides higher...

Superior Quantitative EMCCD Imaging #1 Bias stability – The imaging stability of the EMCCD camera can be assessed by measuring its output with no light falling on the sensor and measuring the slope of the average intensity. The slope of the average intensity value of a 200 frame sequence (where y=mx+b of the least squares fit) is measured. #2 Gain stability – The actual amount of EM Gain applied on each image in a stream of images can vary depending on many electrical engineering factors. The slope of the average intensity value of a 200 frame sequence (where y=mx+b of the least squares fit)...