Evolve™ 512 Delta Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES The new Evolve™ 512 EMCCD Camera Now even faster with 67.5 frames per second Fastest 512 x 512 EMCCD camera on the market 16 x 16-μm pixels Available with Exclusive Primary applications: Super-Resolution Microscopy Quantitative FRET Technology TIRF Multiprobe Experiments Ratiometric Ion Imaging Spinning Disc Confocal Microscopy Lowest read noise available for a high-speed EMCCD camera Ideal for sophisticated researcher and multi-user labs Superb electron multiplication (EM) gain and bias stability Most accurate and fastest EM calibration technique in the industry Available with exclusive eXcelon™ technology Backed by Photometrics’ worldwide support team Features Benefits EM gain Very high sensitivity, low-noise, impact-ionization process Effectively reduces read noise < 1e- Back-illuminated EMCCD Optimized field of view and highest sensitivity Intelligent FPGA design Self-calibrating linearization ensures truly quantitative data consistently, time and again Ensures bias stability to guarantee a stable background Most accurate and precise integrated EM Calibration routine using a highly stable integrated light source Calibrates your EM gain in less than three minutes. PAR feedback system (Photometrics Active Regulation) Delivers unsurpassed EM gain stability for outstanding signal fidelity ACE technology (Advanced Clocking Enhancement) Provides lowest noise floor and minimizes generation of spurious charge and background events Excellent for high-speed image acquisition, 67.5 full frames/sec at 20MHz, 1099 frames/s @ 16x16 pixel ROI, 4x4 binning Wide dynamic range allows detection of bright and dim signals in the same image Frame-transfer EMCCD 100% duty cycle to collect continuous data, no mechanical shutter required Easily attaches to microscopes, standard lenses or optical equipment Universally accepted interface that provides high-bandwidth, uninterrupted data transfer with no dropped frames Windows® XP/7 PVCam® Driver Established driver supported by numerous third-party imaging software packages High Speed Triggering Precise integration with complex systems, controlling illumination, shutters and filter wheels, and other triggered devices Exclusive eXcelon Technology (optional) CCD97 or CCD97-X (with eXcelon technology), highest available quantum efficiency (>90% peak QE) 512 x 512 imaging array 16 x 16-μm pixels Enhanced QE in Blue and near IR wavelengths Anti-Etaloning in near IR wavelengths ©2013 Photometrics. All rights reserved.

Evolve™ 512 Delta Datasheet Superior Quantitative EMCCD Imaging Specifications Read noise (e- rms) 20 MHz EM Port 10 MHz EM Port Pixel well depth Active area Gain register Bias stability A measurement of the camera stability when no light hits the sensor. A slope of zero would be ideal. See footnote #1. Field uniformity The image quality of the EMCCD is assessed for gradients. A complete lack of any gradient (i.e a flat image) would provide a numerical value of 1.00 See footnote #2. Stabilized cooling temperature Air cooled (@ ambient air 20˚C) - Standard Liquid cooled (@ ambient air 20˚C) -...

Evolve™ 512 Delta Datasheet Superior Quantitative EMCCD Imaging 21050 21000 Bias Stability 20MHz Gain 2 Multiplication Gain vs. Slider Value Multiplication Gain vs. Slider Value Bias Stability 20MHz Gain 2 Note: Actual data (See footnote #7.) Actual Multiplication Gain (X) (X) Actual Multiplication Gain Note: Actual data. EM Gain Stability 20MHz Gain 2 @ 350X (Frames per second) Note: Frame rates are measured at 20 MHz with 0-second exposure times. Actual Multiplication Gain (X) Note: Actual data from camera. Exclusive eXcelon Technology (optional) Multiplication Gain vs. Slider Value Standard...

Evolve™ 512 Delta Datasheet Superior Quantitative EMCCD Imaging Footnotes #1 Bias stability – The imaging stability of the EMCCD camera can be assessed by measuring its output with no light falling on the sensor and measuring the slope of the average intensity. The slope of the average intensity value of a 200 frame sequence (where y=mx+b of the least squares fit) is measured. #2 Field uniformity – Specification was obtained using the following formula: σ(bias)/(σ(bias1-bias2)*.707) ≤ 1.15 #3 Dark current – This is measured in a traditional manner (as with all CCD cameras) by taking a long integration...