Photometrics EMCCD Cameras
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FORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIE EMCCD Cameras

Evolve™ 512 Delta Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Primary applications: Super-Resolution Microscopy Quantitative FRET Multiprobe Experiments Spinning Disc Confocal Microscopy Available with Exclusive The new Evolve™ 512 EMCCD Camera ► Fastest 512 x 512 EMCCD camera on the market ► Lowest read noise available for an EMCCD camera ► Ideal for sophisticated researcher and multi-user labs ► Superb electron multiplication (EM) gain and bias stability ► Most accurate EM calibration technique in the industry ► Available with exclusive eXcelon™ technology ► Backed by...

Superior Quantitative EMCCD Imaging Evolve™ 512 Delta Datasheet Pixel well depth Active area Gain register Bias stability A measurement of the camera stability when no light hits the sensor. A slope of zero would be ideal. See footnote #1. Field uniformity The image quality of the EMCCD is assessed for gradients. A complete lack of any gradient (i.e a flat image) would provide a numerical value of 1.00 See footnote #2. Stabilized cooling temperature Air cooled (@ ambient air 20*C) - Standard -80*C Liquid cooled (@ ambient air 20*C) - Optional -85*C Dark current Background events (20 MHz,...

21400 21350 21300 21250 21200 21150 21100 Evolve™ 512 Delta Datasheet Superior Quantitative EMCCD Imaging 21050 21000 0 20 40 60 80 100 120 140 160 180 200 Bias Stability 20MHz Gain 2 460 21350 21300 y = -0.0022x + 430.04 440 21250 21200 430 21150 420 21100 410 21050 21000 400 0 50 100 150 0 200 Multiplication Gain vs. Slider Value Multiplication Gain vs. Slider Value 1200 1200 1000 40 60 80 100 120 140 160 180 200 Bias Stability 20MHz Gain 2 460 Region 450 440 1000 800 512 x 512 430 256 x 256 1x1 62.5 2x2 126 241.7 400 4x4 240.5 420 600 400 400 200 0 1 101 201 301 401 501 601 701 801 901 1...

Evolve™ 512 Delta Datasheet Superior Quantitative EMCCD Imaging #1 Bias stability – The imaging stability of the EMCCD camera can be assessed by measuring its output with no light falling on the sensor and measuring the slope of the average intensity. The slope of the average intensity value of a 200 frame sequence (where y=mx+b of the least squares fit) is measured. #2 Field uniformity – Specification was obtained using the following formula: σ(bias)/(σ(bias1-bias2)*.707) ≤ 1.15 #3 Dark current – This is measured in a traditional manner (as with all CCD cameras) by taking a long...

HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Primary applications: Quantitative FRET Multiprobe experiments Confocal microscopy Live-cell fluorescence imaging Available with Exclusive ► Smallest, most powerful scientific EMCCD camera on the market ► Most advanced feature set available for low-light applications ► Lowest dark current available for an EMCCD camera ► Lowest read noise available for an EMCCD camera ► Ideal for sophisticated researcher and multi-user labs ► Superb electron multiplication (EM) gain and bias stability ► Most accurate EM calibration technique in the...

Evolve™ 512 Datasheet Superior Quantitative EMCCD Imaging Photometrics Advanced Features Features Benefits Quant-View Allows camera to read out pixel values in terms of electrons measured, thereby enabling user to calculate actual photon flux ® Electrons-per-gray-level selector Allows user to select how many electrons will cause a single gray-level increase in the image data, thus permitting utilization of Quant-View while maintaining full dynamic range of EMCCD Rapid-Cal® 3-minute EM calibration process is most accurate in industry; camera does not need to be detached from microscope; no...

Evolve™ 512 Datasheet Superior Quantitative EMCCD Imaging Bias Stability 10MHz Gain 3 350X 502 Read Noise vs. Slider Value 3.00 500 2.50 Noise e- (rms) 498 496 494 492 490 y = 0.0002x + 494.89 488 1.00 0.50 586 571 556 541 526 511 496 481 466 451 436 421 406 391 376 361 346 331 316 301 286 271 256 241 226 211 196 181 166 151 136 91 1.50 0.00 121 76 106 61 46 1 16 484 31 Series1 Linear (Series1) 486 2.00 1 101 201 301 501 601 701 801 901 Note: Actual data, not theoretical. Region Multiplication Gain vs. Slider Value 1200 512 x 512 600 400 200 1 101 201 301 401 501 601 701 801 128 x 128 64 x...

Superior Quantitative EMCCD Imaging Evolve™ 512 Datasheet #1 Bias stability – The imaging stability of the EMCCD camera can be assessed by measuring its output with no light falling on the sensor and measuring the slope of the average intensity. The slope of the average intensity value of a 200 frame sequence (where y=mx+b of the least squares fit) is measured. #2 Gain stability – The actual amount of EM Gain applied on each image in a stream of images can vary depending on many electrical engineering factors. The slope of the average intensity value of a 200 frame sequence (where y=mx+b of...

HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Primary applications: Calcium Imaging Cell Physiology Live Cell Microscopy Single Molecule Fluorescence Available with Exclusive ► Smallest, most powerful scientific EMCCD camera on the market ► Most advanced feature set available for low-light applications ► Lowest dark current available for an EMCCD camera ► Lowest read noise available for an EMCCD camera ► Superb electron multiplication (EM) gain and bias stability ► Most accurate EM calibration technique in the industry ► Available with exclusive eXcelon™ technology ► Backed by...

Evolve™ 128 Datasheet Superior Quantitative EMCCD Imaging Photometrics Advanced Features Features Benefits Quant-View Allows camera to read out pixel values in terms of electrons measured, thereby enabling user to calculate actual photon flux ® Electrons-per-gray-level selector Allows user to select how many electrons will cause a single gray-level increase in the image data, thus permitting utilization of Quant-View while maintaining full dynamic range of EMCCD Rapid-Cal® 3-minute EM calibration process is most accurate in industry; camera does not need to be detached from microscope; no...