DC2 Two-Channel, Full-Field, Simultaneous-Imaging System

Product catalog summary

Overview: The Photometrics DC2™ is a high-performance dual-channel, full-field, simultaneous-imaging system designed for life sciences applications. It enables simultaneous acquisition of two full-field emission images, which can be separated by wavelength, polarization, or amplitude, enhancing scanning speeds in high-content screening applications.

Key Features:

Simultaneous imaging of two channels based on distinct wavelengths or polarizations.
Improved adjustment control for easier image alignment and exchangeable filter cube for multiple applications.
Bypass mode for directing all emission light to a single camera.
Integrated, adjustable CCD mask to minimize ghosting and independent focus adjustment for each camera.
Compatible with many Photometrics® and QImaging® cameras, though compatibility testing is recommended for certain microscope configurations.

Specifications:

Wavelength sensitivity: 400 to 750 nm.
Efficiency per image: 88 to 92%.
Operating temperature: 10 to 37˚C.
Detector attachment: C-mount (male); Front attachment: C-mount (female).
Dimensions: 6.5” width x 8.5” height; Weight: 4.8 lbs.
Filter specifications: Emission/barrier, neutral density, polarization; 1” max diameter; 0.39” max thickness.

Applications:

Fluorescence resonance energy transfer (FRET) imaging.
Calcium imaging with fluo-3/Fura Red™ or dual-emission indo-1 imaging.
Fluorescence polarization/anisotropy imaging and simultaneous fluorescence/DIC imaging.
Drug discovery with Cy3/Cy5 and single-molecule fluorescence (SMF) imaging.
pH imaging with SNARF and multiwavelength total internal reflection fluorescence (TIRF) imaging.
Voltage sensing with di-4-ANEPPS and fluorescence in situ hybridization (FISH) imaging.
cAMP imaging with FICRhR and multichannel confocal microscopy with spinning-disk confocal.

Additional Information: The datasheet includes patent information and contact details for Photometrics. Note that all specifications are typical and subject to change.

Catalog excerpts

DC2 Two-Channel, Full-Field, Simultaneous-Imaging System-1

Photometrics DC2™ Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Dual-Channel, Full-Field, Simultaneous-Imaging System The Photometrics® DC2™ enables two-channel simultaneous imaging based on two distinct wavelengths or polarizations. Each channel is projected onto an individual CCD. Simultaneous multichannel imaging is essential to achieve quantitative emission ratiometric imaging. Features • Simultaneous acquisition of two full-field emission images Green Channel • Emission can be separated by wavelength, polarization, or amplitude • Increases scanning speeds by a factor of two in high-content screening applications • Easily mounts to most microscopes • Improved adjustment control enables easier image alignment • Exchangeable filter cube allows multiple applications to be run with minimal realignment • Bypass mode allows user to send all of the emission light to a single camera • Integrated, adjustable CCD mask minimizes ghosting • Independent focus adjustment for each camera • Works with many Photometrics® and QImaging® cameras* *As some combinations of microscopes and objectives, particularly in some newer Olympus and Zeiss stand/objective configurations, have displayed vignetting, it is recommended that the DC2 be tested for compatibility with your system. Please contact your local representative to verify compatibility with specific cameras. PM-DS-015 Rev B4 ©2013 Photometrics. All rights reserved. Color Overlay

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DC2 Two-Channel, Full-Field, Simultaneous-Imaging System-2

Dual-Channel, Full-Field, Simultaneous-Imaging System Wavelength sensitivity Operation temperature Detector attachment C-mount (male) Front attachment C-mount (female) External mounting option ¼-20 tapped hole on back of unit 6.5” at widest point x 8.5” height Emission/barrier, neutral density, polarization; 1” (25.4-mm) max diameter; 0.39” (9.9-mm) max thickness • Fluorescence resonance energy transfer (FRET) imaging • Calcium imaging with fluo-3/Fura Red™ (Molecular Probes) or dual-emission indo-1 imaging • Fluorescence polarization/anisotropy imaging • Simultaneous fluorescence/DIC imaging...

Open the catalog to page 2