Photometrics DC2™ Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES Dual-Channel, Full-Field, Simultaneous-Imaging System The Photometrics® DC2™ enables two-channel simultaneous imaging based on two distinct wavelengths or polarizations. Each channel is projected onto an individual CCD. Simultaneous multichannel imaging is essential to achieve quantitative emission ratiometric imaging. Features • Simultaneous acquisition of two full-field emission images Green Channel • Emission can be separated by wavelength, polarization, or amplitude • Increases scanning speeds by a factor of two in high-content screening applications • Easily mounts to most microscopes • Improved adjustment control enables easier image alignment • Exchangeable filter cube allows multiple applications to be run with minimal realignment • Bypass mode allows user to send all of the emission light to a single camera • Integrated, adjustable CCD mask minimizes ghosting • Independent focus adjustment for each camera • Works with many Photometrics® and QImaging® cameras* *As some combinations of microscopes and objectives, particularly in some newer Olympus and Zeiss stand/objective configurations, have displayed vignetting, it is recommended that the DC2 be tested for compatibility with your system. Please contact your local representative to verify compatibility with specific cameras. PM-DS-015 Rev B4 ©2013 Photometrics. All rights reserved. Color Overlay

Dual-Channel, Full-Field, Simultaneous-Imaging System Wavelength sensitivity Operation temperature Detector attachment C-mount (male) Front attachment C-mount (female) External mounting option ¼-20 tapped hole on back of unit 6.5” at widest point x 8.5” height Emission/barrier, neutral density, polarization; 1” (25.4-mm) max diameter; 0.39” (9.9-mm) max thickness • Fluorescence resonance energy transfer (FRET) imaging • Calcium imaging with fluo-3/Fura Red™ (Molecular Probes) or dual-emission indo-1 imaging • Fluorescence polarization/anisotropy imaging • Simultaneous fluorescence/DIC imaging...