CoolSNAP ™ K4 Monochrome Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES 2048 x 2048 imaging array 7.4 x 7.4-μm pixels The CoolSNAP™ K4 Monochrome camera from Photometrics® delivers high-resolution imaging for low-light scientific applications that require a large field of view. This cooled CCD camera provides 12-bit digitization at 20 MHz. The large format of the CCD allows the user to image the microscope’s whole field of view, while the small pixel size is ideally matched to the resolution limit of the microscope. The 4-megapixel detector enables very fine image detail to be resolved, yet the pixels can be easily binned to improve sensitivity. Advanced interlinetransfer CCD technology provides high quantum efficiency. Primary applications Histology Immunofluorescence FISH Fixed-cell GFP imaging 3D deconvolution Multiple-wavelength FRET High-resolution time-lapse microscopy High-speed, high-sensitivity image capture Resolves fine detail Ideally matched to optical microscope Eliminates camera vibration and facilitates fast triggering Flexible binning and readout Increases light sensitivity while increasing the frame rate Quantifies bright and dim signals in the same image Thermoelectric cooling Long integration times for higher sensitivity Enhanced quantum efficiency Provides higher sensitivity than typical interline cameras Easily attaches to microscopes, standard lenses, or optical equipment Acquisition software Captures, analyzes, and saves high-resolution images High-bandwidth, uninterrupted data transfer PVCam® Circular buffers Device sequencing Supported by numerous third-party software packages Real-time focus Precise integration with shutters, filter wheels, etc. Compatible with Windows® XP/Vista 32, Mac OS X, and Linux® (kernel versions 2.4 and 2.6.8) ©2010 Photometrics. All rights reserved.

CoolSNAP ™ K4 Monochrome Datasheet Quantum Efciency (%) Note: Frame rates are measured at 20 MHz with 0-second exposure times. Kodak® KAI-4020M; interline-transfer, progressive-scan device with microlenses 2048 x 2048 imaging array 7.4 x 7.4-μm pixels 15.16 x 15.16-mm imaging area (optically centered) Linear full well 30,000 e- (single pixel) 60,000 e- (2 x 2 binned pixel) Read noise Digitizer type Dark current Operating environment 0 to 30°C ambient, 0 to 80% relative humidity noncondensing TTL (trigger/status): trigger, invert, inhibit, exposing, interline shift, frame readout 8-bit TTL (programmable)...