Cascade II:512 The Most Sensitive EMCCD Camera for Bioscience
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Cascade® II 512 Datasheet HIGH PERFORMANCE EMCCD & CCD CAMERAS FOR LIFE SCIENCES 512 x 512 imaging array 16 x 16-μm pixels Primary applications Ultra-low-light fluorescence Total internal reflection fluorescence (TIRF) Single-molecule fluorescence Spectral imaging The Photometrics® Cascade® II:512 is the only EMCCD microscopy camera in the world to offer -80˚C cooling without LN2 or water! This 16-bit, high-resolution camera uses its deep thermoelectric cooling to maximize gain and minimize dark current. A stainless-steel vacuum chamber (with all-metal seals) houses a high-QE, back-illuminated, frame-transfer EMCCD. The camera’s exclusive vacuum technology is so robust it carries a lifetime guarantee. Low-noise, impact-ionization process provides very high sensitivity Back-illuminated EMCCD Highest available quantum efficiency (>90% peak QE) Deep cooling Thermoelectric cooling to -80˚C minimizes dark current and allows long exposure times No need for a bulky chilled-water circulator or cryogenic compressor, both of which are prone to leaks, blockages, and condensation Lifetime vacuum Permanent, all-metal vacuum seals guaranteed for lifetime of camera Maintenance-free operation 512 x 512 imaging array 16 x 16-μm pixels Good field of view and sensitivity Good resolution Excellent for high-speed image visualization Perfect for high-precision photometry Dual amplifiers Select readout mode via software: (1) optimal high-speed / high-sensitivity performance (2) optimal wide-dynamic-range performance Wide dynamic range allows detection of bright and dim signals in the same image Frame-transfer EMCCD 100% duty cycle for continuous data collection No mechanical shutter required Single optical window Single vacuum window is the only optical surface between incident light and EMCCD surface No light loss from multiple optical surfaces Easily attaches to microscopes, standard lenses, or optical equipment Acquisition software Captures, analyzes, and saves high-resolution images High-bandwidth, uninterrupted data transfer PVCam® Circular buffers Device sequencing Supported by numerous third-party software packages Real-time focus Precise integration with shutters, filter wheels, etc. Compatible with Windows® XP/Vista 32, Mac OS X, and Linux® (kernel versions 2.4 and 2.6.8) ©2012 Photometrics. All rights reserved.

EMCCD Technology Quantum Efciency (%) Note: Frame rates are measured at 10 MHz with 0-second exposure times. EMCCD image sensor e2v CCD97; back-illuminated, frame-transfer CCD with EM gain EMCCD format 512 x 512 imaging pixels; 16 x 16-μm pixels; 8.2 x 8.2-mm imaging area (optically centered) Linear full well single pixel output node Digitizer type 16 bits @ 10 MHz, 5 MHz, and 1 MHz “EM gain” amplifier (port #1) “Traditional” amplifier (port #2) Read noise Read noise effectively reduced to <1 e- rms with EM gain enabled EM gain 1 to 1,000x (typical) Controlled via software Parallel...