H I G H - P E R F O R M A N C E E M C C D & C C D C A M E R A S F O R L I F E S C I E N C E S Primary applications Neurosciences Single-molecule fluorescence Live-cell microscopy Spinning-disc confocal microscopy 128 x 128 imaging array 24 x 24-ìm pixels Features Benefits EM gain Very high sensitivity Low-noise, impact-ionization process Back-illuminated EMCCD Highest available quantum efficiency (>90% peak QE) 128 x 128 imaging array 24 x 24-ìm pixels Small array facilitates fast readout Good resolution 12-MHz readout Excellent for live-cell microscopy 16-bit digitization Wide dynamic range allows detection of bright and dim signals in the same image Frame-transfer EMCCD 100% duty cycle to collect continuous data No mechanical shutter required Thermoelectric cooling Detector cooled to reduce background for high sensitivity C-mount Easily attaches to microscopes, standard lenses, or optical equipment Acquisition software Captures, analyzes, and saves high-resolution images PCI interface High-bandwidth, uninterrupted data transfer PVCAM® Circular buffers Device sequencing Supported by numerous third-party software packages Real-time focus Precise integration with shutters, filter wheels, etc. Compatible with Windows® XP/Vista 32, Mac OS X, and Linux® (kernel versions 2.4 and 2.6.8) The Cascade:128+ camera from Photometrics® uses a high-QE, back-illuminated CCD with EM gain to provide extraordinary sensitivity for low-light-level, live-cell microscopy applications. Its thermoelectrically cooled detection array features square, 24-ìm pixels in a 128 x 128, frame-transfer format. The state-of-the-art camera can collect more than 500 full frames of true 16-bit data per second — faster frame rates are achievable via subregion readout or binning. This unprecedented combination of speed and sensitivity makes the Cascade:128+ a perfect choice for neuroscience applications and single-molecule fluorescence (SMF) imaging.

PM-DS-002-C0 Cascade:128+ EMCCD Technology [email protected] USA +1.520.889.9933 Asia Pacific +65.6841.2094 France +33.1.60.86.03.65 Germany +49.89.660.779.3 Japan +81.3.5639.2731 UK +44.1628.890858 www.photomet.com 200 300 400 500 600 700 800 900 1000 1100 100 90 80 70 60 50 40 30 20 10 0 Wavelength (nm) Quantum Efficiency (%) Region Binning 128 x 128 64 x 64 32 x 32 1 x 1 510 926 1684 2 x 2 926 1610 2732 3 x 3 1272 2227 3676 4 x 4 1656 2703 4149 (Frames per second) Note: Frame rates are measured at 12 MHz with 0-second exposure times. Specifications EMCCD image sensor e2v CCD60; back-illuminated,...