?AW -xt SYSTEM Prep and Load Platform Specifications for PAL-xt Systems Valid for PAL-xt System models only Revised March 2013 | V5 PAL is a registered trademark of CTC Analytics AG | Switzerland

Gas Chromatographic Test: High Split Ratio | Retention Gap | Solvent iso-Octane 4 Gas Chromatographic Test: Split Mode | Analytical Column | Alkene Test Mix 5 Gas Chromatographic Headspace Test: High Split Ratio | Retention Gap | Solvent iso-Octane 6 HPLC Chromatographic Test: Loop Overfill and Partial Loop Filling Standard Injection Technique 7 HPLC Chromatographic Test: Loop Overfill and Partial Loop Filling DLW Injection Technique 8 HPLC Chromatographic Test: Loop Overfill and Partial Loop Filling DLW-2 Low volume Injection 9 Formulas used to calculate the Specification Values...

Syringe Volume Specification Test Conditions 10 pi Repeatability 15 times 8 pL Water transferred into Relative Standard Deviation target vial. s-rel :_0.20 % 100 pL Repeatability 100 pL Water transferred in a target vial. Relative Standard Deviation s-rel :_0.15 % Linearity 6 levels and 3 replicates of each level. R: >0.9999 1000 pL Repeatability 500 pL Water transferred in a target vial. Relative Standard Deviation s-rel : 0.10 % Linearity 6 levels and 3 replicates of each level. R: > 0.9999 2500 pL Repeatability 1000 pL Water transferred in a target Relative...

Specification: Repeatability: Relative Standard Deviation s-rel: 0.30 % Carry-over: not applicable General Remarks: Number of specimens for Repeatability Tests: n = 20 Carry-over: Test: Remaining solvent in syringe can not be eliminated from injection to injection. Test Conditions: Test Sample: iso-Octane puriss., 2 mL vials filled to 50 % of nominal content Syringe: Hamilton pNo.: 203205 | CTC Analytics PNo.: SyrC L10-26S-AS PAL-xt Parameter Settings: CYCLE GC-Inj Fill Strokes 5 SYRINGE10 |jlPullup Del5 s Sample Volume1.0 p.LInject toGC Inj1 Air Volume 0.5 |jl Inject...

Specification: Repeatability: Relative Standard Deviation s-rel: 0.80 % General Remarks: Number of specimens for Repeatability Tests: n = 8 Repeatability determination based on C14 Peak Carry-over Test: Peak < 1 area count, not detectable. Test Conditions: Test Sample: Alkane Test Mix C14,G5,G6 | 0.3 p.g dissolved in iso-Octane. 2 mL vials filled to 50 % of nominal content Syringe: Hamilton PNo.: 203205 | CTC Analytics PNo.: SyrC L10-26S-AS PAL-xt Parameter Settings: CYCLE GC-Inj Fill Strokes 5 Sample Volume 1.0 p.L Inject to GC Inj1 Air Volume 0.5 |jl Inject Speed 50...

Specification: Repeatability: Relative Standard Deviation s-rel: < 1.00 % General Remarks: Number of specimens for Repeatability Tests: n = 7 10 |jl are measured by using a glass capillary, Drummond Microcaps (Supplier Supelco, PNo. P-1924) Test Conditions: Test Sample: Iso-Octane, puriss., Microcaps Glass Capillary hold with Tweezers, filled and put into 20 mL Headspace vial, sealed immediately. Syringe: Hamilton PNo.: 203084 | CTC Analytics PNo.: SyrC HS 2.5 -23-5 PAL-xt Parameter Settings: CYCLE HS-Inj Fill Speed 100 |jl/s Sample Volume 500 |jl Inject to GC Inj1 Incubat...

-xt SYSTEM | HTC-xt | HTS-xt HPLC Chromatographic Test: Loop Overfill and Partial Loop Filling Standard Injection Technique Specification: Syringe Volume Specification Test Conditions 100 pL Repeatability Loop Overfill Loop size 20 pi Relative Standard Deviation Loop fill volume: 80 pL 100 pL Repeatability Partial Loop Filling Loop size 20 pL Relative Standard Deviation Loop fill volume: 10 pL 100 pL Linearity 5 levels and 3 replicates of each level Without Degassing and Column Heater Column: Zorbax XDB-C8, Length: 150 mm, ID 4.6 mm, 5 pm particle size, end-capped Suppliers:...

Syringe Volume Specification Test Conditions 100 |jl Repeatability Loop Overfill Loop size 2 |j.L Relative Standard Deviation Loop fill volume: 7 |j.L s-rel : 0.50 % 100 |jl Repeatability Partial Loop Filling Loop size 20 |j.L Relative Standard Deviation Loop fill volume: 10 |j.L s-rel : 0.50 % 100 |jl Carry-over: Test Blank after full loop injection < 40 ppm Loop size 2 |jl Loop fill volume: 7 |jl General Remarks: Number of specimens for Repeatability Tests: n = 10 for Loop Overfill Number of specimens for Repeatability Tests: n = 10 for Partial Loop...

HPLC Chromatographic Test: Loop Overfill and Partial Loop Filling DLW-2 Low volume Injection Specification: Syringe Volume Specification Test Conditions 100 |jl Repeatability Loop Overfill Loop size 5 |jl Relative Standard Deviation Loop fill volume: 15 |j.L s-rel : 0.5 % 100 |j.L Repeatability Loop Overfill Loop size 2 |j.L Relative Standard Deviation Loop fill volume: 7 |jl s-rel : 0.5 % 100 |jl Repeatability Partial Loop Filling Loop size 5 |jl Relative Standard Deviation Loop fill volume: 2.5 |jl s-rel : 0.5 % 100 |jl Repeatability Partial...

Mean Value (average). Average of measured area counts. x = Mean Value of area counts. Xi = Peak area counts, i th observation of n n = Number of injections, calibration points. Standard Deviation Relative Standard Deviation x RSD = Relative Standard Deviation