InSight X3+ and InSight X3 Widely Tunable Ultrafast Lasers For Multiphoton Imaging The InSight® X3+™ and InSight X3™ are built on Spectra-Physics’ industry leading InSight platform designed for maximum performance and reliability. The InSight X3+ delivers 50% greater power across the tuning range for deeper imaging and more complex experiments. The InSight X3+ and InSight X3 lasers are built on the proven InSight platform with hundreds of peer-reviewed publications, the largest installed base in the market, and demonstrated high reliability. The InSight platform is specifically designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences. Based on patented technology1, InSight features a broad 680 nm to 1300 nm continuous, gap free, tuning curve from a single beam with nearly double the tuning range of legacy Ti:Sapphire lasers. The InSight is designed with the peak of the tuning curve centered in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry. The InSight X3+ additionally provides the highest powers at the longest wavelengths of 1280–1300 nm for excitation of far red fluorophores such as Alexa-680. The InSight Advantage • High power across entire tuning range with the industry’s highest power at the multiphoton imaging wavelengths between 900 and 1300 nm • Field proven with the largest installed base in the market, hundreds of peer-reviewed publications and demonstrated high reliability • Broad tuning range (680–1300 nm) for maximum flexibility • Dual output at 1045 nm with optional pre-compensation for simultaneous two color excitation • Short pulse width and highest peak power for maximum fluorescence • Integrated DeepSee dispersion pre-compensation to deliver short pulses to the sample • Ideal beam characteristics optimized for multiphoton imaging Applications • Multiphoton microscopy • Two-Photon Excited Fluorescence (TPEF) • Multimodal imaging - Coherent anti-stokes Raman Spectroscopy (CARS) - Stimulated Raman spectroscopy (SRS) - Second harmonic generation (SHG) - Third harmonic generation (THG) • Optogenetics • Non-linear spectroscopy • Time-resolved photoluminescence • Optical computed tomography • Terahe

InSight X3+ and InSight X3 With Spectra-Physics’ integrated and patented DeepSee™, the industry standard dispersion pre-compensation module, the short femtosecond pulses are optimally delivered through a microscope to the sample providing maximum fluorescence and penetration depth. The InSight has exceptional beam pointing stability, beam quality, output power stability and noise, with fast wavelength tuning. When equipped with the fixed 1045 nm dual beam option, the InSight platform fully supports the diverse needs of multimodal imaging. The two synchronized output beams enable easy simultaneous...

InSight X3+ and InSight X3 Specifications1, 11 InSight X3+ Output Characteristic Main Output Dual Output Main Output Dual Output Tunable Range Beam diameter(1/e²)3 Beam divergence, full angle3 Beam Roundness Beam Astigmatism³ Beam pointing stability9 Tuning speed10 Stability5 Environmental Requirements Altitude (m) Temperature, Operating Relative Humidity, Operating Temperature, Storage Relative Humidity, Storage Cooled Water Temperature in Closed-loop Chiller Up to 2000 M 20–25 °C Maximum 75% non-condensing up to 25°C 15–35°C Maximum 65% for 15–35°C 21°C, Typical8 Electrical Requirements Power...

Dual Option Output Main Tunable Output Front View Back View Dimensions in inch (mm) InSight X3+ and InSight X3 Dimensions 3635 Peterson Way, Santa Clara, CA 95054, USA PHONE: 1-800-775-5273 1-408-980-4300 FAX: 1-408-980-6921 Belgium +32-(0)0800-11 257 China +86-10-6267-0065 France +33-(0)1-60-91-68-68 Germany / Austria / Switzerland +49-(0)6151-708-0 Japan +81-3-3794-5511 [email protected] [email protected] [email protected] [email protected] [email protected] Korea Netherlands Singapore Taiwan United Kingdom EMAIL: [email protected] +82-31-8021-1600 [email protected]...