Reproducibility Studies NanoDrop 2000/2000c
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Reproducibility Studies Introduction The precision or reproducibility of the Thermo Scientific NanoDrop 2000 Spectrophotometer was assessed measuring 10 individual aliquots of 8 concentrations of herring sperm dsDNA. Between each measurement, the sample was removed with a dry, lint free lab wipe and a fresh aliquot of dsDNA was analyzed. A second reproducibility study on the NanoDrop™ 2000c was conducted using 8 different concentrations of herring sperm DNA diluted in azide buffer. Following a blank measurement with the buffer, 5 separate aliquots of the DNA sample solutions were measured using a 1 cm quartz cuvette. NanoDrop 2000/2000c pedestal Measurement 1 Measurement 2 Measurement 3 Measurement 4 Measurement 5 Measurement 6 Measurement 7 Measurement 8 Measurement 9 Measurement 10 Average (ng/uL) St Dev (ng/uL) % CV Reproducibility 2 - 100 ng/uL SD: ±2ng/uL >100 ng/uL to ~15000 ng/uL %CV: 2% NanoDrop 2000c cuvette (1cm quartz ) Measurement 1 Measurement 2 Measurement 3 Measurement 4 Measurement 5 Average (ng/uL) St Dev (ng/uL) Reproducibility 0.4 ng/uL to 10 ng/uL SD: ±0.15 ng/uL Rev 3/09 Thermo Fisher Scientific - NanoDrop products Technical support: info@nanodrop.com