High Signal-to-Noise Fluorescence Filters
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558 Phone: 1-800-222-6440 • Fax: 1-949-253-1680 O p t i c s ACCESSORIES ULTRAFAST LASER OPTICS HIGH-ENERGY OPTICS DIFFRACTION GRATINGS POLARIZATION OPTICS OPTICAL FILTERS BEAMSPLITTERS WINDOWS Newport's High Signal-to-Noise Fluorescence Filters provide a custom defined narrow bandpass that has been designed based upon the absorption and emission spectra of the specific fluorophore of intended use. With a typical non-paired signal-to-noise ratio of 100,000:1, they can be paired with any other filter in the series with excellent crosstalk suppression. Developed as XM Fluorescence Filters and manufactured in Newport's Franklin, MA facility, these filters are a key enabling technology in numerous fluorescence based research and clinical instruments. Typical applications include instrumentation for flow cytometry, drug discovery, genetic analysis, and other fluorescence-based instrumentation. Specifications Diameter 25.4 mm+0/-0.5 mm Minimum Active Area 20.2 mm Surface Quality 80-50 scratch-dig; F/F per MIL-F-48616 Thickness 1.0 to 9.0 mm Effective Index of Refraction ne 1.5 Wavelength Shift with Temperature 0.01 nm/°C Temperature Range -50°C to +100 °C 100% 10% 1.0% 0.1% 0.01% 0.001% 0.0001% . . 100 10-1 10-3 10-2 10-5 10-4 10-6 PERCENT TRANSMITTANCE 50% Tpk 10% Tpk 0.5 BW 0.1 BW 0 . PEAK TRANSMITTANCE Tpk . . 1% Tpk . . . 0.01 BW . FWHM 1.1 FWHM 1.2 FWHM 0.00001% 10-7 10-8 10-10 10-9 0.1% Tpk . 0.01% Tpk . 1.4 FWHM 1.7 FWHM 0.001 BW . 0.0001 BW . X-RAY WAVELENGTH () FAR I.R. . . Bandshape specifications and marker wavelengths are provided as approximate reference data only Integrated Cross-Talk Data for Fluorophore-Specific Filter Pairs Dye Wavelength of Max Absorption Wavelength of Max Emission Excitation Filter Emission Filter Integrated Cross-Talk (Reference Only) Aminomethylcoumarin (AMC) 345 442 10XM35-360 10XM35-430 1,000,000:1 DAPI Hoechst 33258 4-Methylumbeliferone CPM 358 351 360 385 458 455 450 471 10XM35-360 10XM35-465 100,000,000:1 Lucifer Yellow 430 540 10XM35-430 10XM25-535 1,000,000:1 Acridine Orange (+RNA) 460 650 10XM35-465 10XM35-625 1,000,000:1 YOYO-1 (+DNA) Fluorescein (FITC) Acridine Orange (+DNA) BODIPY FL Rhodamine 123 491 495 502 503 505 509 519 526 512 528 10XM20-485 10XM25-535 100,000,000:1 Ethidium Bromide (+DNA) 510 595 10XM20-485 10XM35-595 100,000,000:1 Tetramethyrhodamine (TRITC) 552 580 10XM25-530 10XM35-595 10,000,000:1 R-phycoerythrin 565 575 10XM20-485 10XM10-585 1,000,000:1 Ethidium Homodimer-1 Propidium Iodide 528 536 617 617 10XM25-530 10XM35-625 100,000,000:1 7-Aminoactinomycin (7-AAD) 550 645 10XM25-530 10XM40-650 1,000,000,000:1 Texas Red 595 615 10XM20-590 10XM35-635 100,000:1 • High transmission • Excellent spectral blocking • Very low autofluorescence • Excellent paired crosstalk suppression • Patented Stabilife® construction • Superior field life Filter Holders see page 688 High Signal-to-Noise Fluorescence Filters

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High Signal-to-Noise Fluorescence Filters -2

O p t i c s 559 Email: [email protected] • Web: newport.com WINDOWS BEAMSPLITTERS OPTICAL FILTERS POLARIZATION OPTICS DIFFRACTION GRATINGS HIGH-ENERGY OPTICS ULTRAFAST LASER OPTICS ACCESSORIES Ordering Information Center Wavelength (nm) FWHM (nm) Minimum Peak Transmission (%) Signal-to-Noise Ratio Integrated Blocking Range Model 360, +0/-5 35±3.5 45 10,000:1 X-ray to 700 nm 10XM35-360 425, +5/-0 35±3.5 50 100,000:1 X-Ray to 925 nm 10XM35-430 460, +5/-0 35±3.5 50 100,000:1 X-Ray to 925 nm 10XM35-465 485, +0/-5 20±2.0 60 100,000:1 X-Ray to 925 nm 10XM20-485 530, +0/-5 25±2.5 60 100,000:1 X-Ray...

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